Effect of silymarin on the relative gene expressions of some inflammatory cytokines in the liver of CCl4-intoxicated male rats
Abstract The intensive exposure of the liver cells to any type of noxae, such as viruses, drugs, alcohols, and xenobiotics could induce hepatic inflammation through the upregulation of gene expression of several fibrotic and inflammatory mediators. So, our study assessed the role of silymarin on the...
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| Format: | Article |
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Nature Portfolio
2023-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-42250-7 |
| _version_ | 1827711481053970432 |
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| author | Sarah M. El-Kot Wessam Wanas Afaf M. Hafez Nihal A. Mahmoud Amina M. Tolba Abeer H. Younis Gamal El Sayed Huda E. Abdelwahab |
| author_facet | Sarah M. El-Kot Wessam Wanas Afaf M. Hafez Nihal A. Mahmoud Amina M. Tolba Abeer H. Younis Gamal El Sayed Huda E. Abdelwahab |
| author_sort | Sarah M. El-Kot |
| collection | DOAJ |
| description | Abstract The intensive exposure of the liver cells to any type of noxae, such as viruses, drugs, alcohols, and xenobiotics could induce hepatic inflammation through the upregulation of gene expression of several fibrotic and inflammatory mediators. So, our study assessed the role of silymarin on the inflammatory response induced by carbon tetrachloride (CCl4) as an example of xenobiotics on liver tissues in male rats. Forty-eight Wister male rats (weight: 130 ± 10) were housed for 14 days and then divided randomly into six groups: control, SLY: rats received only silymarin orally for 12 weeks (daily), CO: rats were injected with corn oil for 8 weeks (3 times weekly), CCl4: rats were injected with CCl4 solubilized in corn oil for 8 weeks (day by day), Treated: rats received silymarin for 4 weeks after CCl4 injection, Protected: rats received silymarin for 4 weeks before and 8 weeks during CCl4 injection. When the treatment period for the rats was over, they underwent scarification after anesthesia. Then, the sera were extracted from the collected blood for the determination of irisin levels, liver functions, and lipid profiles. Liver tissues were separated for the histopathological examinations, the determination of oxidative stress (OS) parameters content, and the relative gene expression of inflammatory cytokines; nuclear factor kappa (NF)-κB, tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, cyclooxygenase (COX)-2, and transforming growth factor beta (TGF-β). The findings showed that silymarin reduced liver inflammation by overcoming the OS process and inflammatory cytokines production which was stimulated by CCl4. These results were confirmed by histopathology of liver tissues. |
| first_indexed | 2024-03-10T17:55:08Z |
| format | Article |
| id | doaj.art-af58ad35e8ac425681dc86f05195279a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T17:55:08Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-af58ad35e8ac425681dc86f05195279a2023-11-20T09:13:37ZengNature PortfolioScientific Reports2045-23222023-09-0113111210.1038/s41598-023-42250-7Effect of silymarin on the relative gene expressions of some inflammatory cytokines in the liver of CCl4-intoxicated male ratsSarah M. El-Kot0Wessam Wanas1Afaf M. Hafez2Nihal A. Mahmoud3Amina M. Tolba4Abeer H. Younis5Gamal El Sayed6Huda E. Abdelwahab7Biochemistry Department, Faculty of Science, Alexandria UniversityMaterials Science Department, Institute of Graduate Studies and Research, Alexandria UniversityEnvironmental Studies Department, Institute of Graduate Studies and Research, Alexandria UniversityPhysiology Department, Faculty of Medicine (Girls), Al-Azhar UniversityAnatomy Department, Faculty of Medicine (Girls), Al-Azhar UniversityNational Institute of Oceanography and Fisheries (NIOF)Biochemistry Department, Faculty of Science, Alexandria UniversityEnvironmental Studies Department, Institute of Graduate Studies and Research, Alexandria UniversityAbstract The intensive exposure of the liver cells to any type of noxae, such as viruses, drugs, alcohols, and xenobiotics could induce hepatic inflammation through the upregulation of gene expression of several fibrotic and inflammatory mediators. So, our study assessed the role of silymarin on the inflammatory response induced by carbon tetrachloride (CCl4) as an example of xenobiotics on liver tissues in male rats. Forty-eight Wister male rats (weight: 130 ± 10) were housed for 14 days and then divided randomly into six groups: control, SLY: rats received only silymarin orally for 12 weeks (daily), CO: rats were injected with corn oil for 8 weeks (3 times weekly), CCl4: rats were injected with CCl4 solubilized in corn oil for 8 weeks (day by day), Treated: rats received silymarin for 4 weeks after CCl4 injection, Protected: rats received silymarin for 4 weeks before and 8 weeks during CCl4 injection. When the treatment period for the rats was over, they underwent scarification after anesthesia. Then, the sera were extracted from the collected blood for the determination of irisin levels, liver functions, and lipid profiles. Liver tissues were separated for the histopathological examinations, the determination of oxidative stress (OS) parameters content, and the relative gene expression of inflammatory cytokines; nuclear factor kappa (NF)-κB, tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, cyclooxygenase (COX)-2, and transforming growth factor beta (TGF-β). The findings showed that silymarin reduced liver inflammation by overcoming the OS process and inflammatory cytokines production which was stimulated by CCl4. These results were confirmed by histopathology of liver tissues.https://doi.org/10.1038/s41598-023-42250-7 |
| spellingShingle | Sarah M. El-Kot Wessam Wanas Afaf M. Hafez Nihal A. Mahmoud Amina M. Tolba Abeer H. Younis Gamal El Sayed Huda E. Abdelwahab Effect of silymarin on the relative gene expressions of some inflammatory cytokines in the liver of CCl4-intoxicated male rats Scientific Reports |
| title | Effect of silymarin on the relative gene expressions of some inflammatory cytokines in the liver of CCl4-intoxicated male rats |
| title_full | Effect of silymarin on the relative gene expressions of some inflammatory cytokines in the liver of CCl4-intoxicated male rats |
| title_fullStr | Effect of silymarin on the relative gene expressions of some inflammatory cytokines in the liver of CCl4-intoxicated male rats |
| title_full_unstemmed | Effect of silymarin on the relative gene expressions of some inflammatory cytokines in the liver of CCl4-intoxicated male rats |
| title_short | Effect of silymarin on the relative gene expressions of some inflammatory cytokines in the liver of CCl4-intoxicated male rats |
| title_sort | effect of silymarin on the relative gene expressions of some inflammatory cytokines in the liver of ccl4 intoxicated male rats |
| url | https://doi.org/10.1038/s41598-023-42250-7 |
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