Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light
In this study, nanochitosan dots (ChiDs) were synthesized using gamma rays and encapsulated in bacterial cellulose (BC) polymer matrix for antibiofilm potential in photodynamic therapy. The composites were analyzed for structural changes using SEM, AFM, FTIR, XRD, EPR, and porosity measurements. Add...
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| Format: | Article |
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MDPI AG
2024-03-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/15/3/72 |
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| author | Danica Z. Zmejkoski Nemanja M. Zdravković Milica D. Budimir Filimonović Vladimir B. Pavlović Svetlana V. Butulija Dušan D. Milivojević Zoran M. Marković Biljana M. Todorović Marković |
| author_facet | Danica Z. Zmejkoski Nemanja M. Zdravković Milica D. Budimir Filimonović Vladimir B. Pavlović Svetlana V. Butulija Dušan D. Milivojević Zoran M. Marković Biljana M. Todorović Marković |
| author_sort | Danica Z. Zmejkoski |
| collection | DOAJ |
| description | In this study, nanochitosan dots (ChiDs) were synthesized using gamma rays and encapsulated in bacterial cellulose (BC) polymer matrix for antibiofilm potential in photodynamic therapy. The composites were analyzed for structural changes using SEM, AFM, FTIR, XRD, EPR, and porosity measurements. Additionally, ChiD release was assessed. The results showed that the chemical composition remained unaltered, but ChiD agglomerates embedded in BC changed shape (1.5–2.5 µm). Bacterial cellulose fibers became deformed and interconnected, with increased surface roughness and porosity and decreased crystallinity. No singlet oxygen formation was observed, and the total amount of released ChiD was up to 16.10%. Antibiofilm activity was higher under green light, with reductions ranging from 48 to 57% under blue light and 78 to 85% under green light. Methicillin-resistant <i>Staphylococcus aureus</i> was the most sensitive strain. The new photoactive composite hydrogels show promising potential for combating biofilm-related infections. |
| first_indexed | 2024-04-24T18:08:23Z |
| format | Article |
| id | doaj.art-af58c74996544352b227e706a11ef519 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-04-24T18:08:23Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-af58c74996544352b227e706a11ef5192024-03-27T13:48:39ZengMDPI AGJournal of Functional Biomaterials2079-49832024-03-011537210.3390/jfb15030072Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green LightDanica Z. Zmejkoski0Nemanja M. Zdravković1Milica D. Budimir Filimonović2Vladimir B. Pavlović3Svetlana V. Butulija4Dušan D. Milivojević5Zoran M. Marković6Biljana M. Todorović Marković7Vinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, 11001 Belgrade, SerbiaScientific Institute for Veterinary Medicine of Serbia, Janisa Janulisa 14, 11107 Belgrade, SerbiaVinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, 11001 Belgrade, SerbiaFaculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade, SerbiaVinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, 11001 Belgrade, SerbiaVinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, 11001 Belgrade, SerbiaVinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, 11001 Belgrade, SerbiaVinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, 11001 Belgrade, SerbiaIn this study, nanochitosan dots (ChiDs) were synthesized using gamma rays and encapsulated in bacterial cellulose (BC) polymer matrix for antibiofilm potential in photodynamic therapy. The composites were analyzed for structural changes using SEM, AFM, FTIR, XRD, EPR, and porosity measurements. Additionally, ChiD release was assessed. The results showed that the chemical composition remained unaltered, but ChiD agglomerates embedded in BC changed shape (1.5–2.5 µm). Bacterial cellulose fibers became deformed and interconnected, with increased surface roughness and porosity and decreased crystallinity. No singlet oxygen formation was observed, and the total amount of released ChiD was up to 16.10%. Antibiofilm activity was higher under green light, with reductions ranging from 48 to 57% under blue light and 78 to 85% under green light. Methicillin-resistant <i>Staphylococcus aureus</i> was the most sensitive strain. The new photoactive composite hydrogels show promising potential for combating biofilm-related infections.https://www.mdpi.com/2079-4983/15/3/72chitosan nanoparticlesbacterial cellulosephotoactive therapyantibiofilmnanocomposite hydrogelsblue light and green light |
| spellingShingle | Danica Z. Zmejkoski Nemanja M. Zdravković Milica D. Budimir Filimonović Vladimir B. Pavlović Svetlana V. Butulija Dušan D. Milivojević Zoran M. Marković Biljana M. Todorović Marković Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light Journal of Functional Biomaterials chitosan nanoparticles bacterial cellulose photoactive therapy antibiofilm nanocomposite hydrogels blue light and green light |
| title | Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light |
| title_full | Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light |
| title_fullStr | Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light |
| title_full_unstemmed | Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light |
| title_short | Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light |
| title_sort | reduction in pathogenic biofilms by the photoactive composite of bacterial cellulose and nanochitosan dots under blue and green light |
| topic | chitosan nanoparticles bacterial cellulose photoactive therapy antibiofilm nanocomposite hydrogels blue light and green light |
| url | https://www.mdpi.com/2079-4983/15/3/72 |
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