Enhanced Production of (<i>R</i>)-3-Hydroxybutyrate Oligomers by Coexpression of Molecular Chaperones in Recombinant <i>Escherichia coli</i> Harboring a Polyhydroxyalkanoate Synthase Derived from <i>Bacillus cereus</i> YB-4
The biodegradable polyester poly-(<i>R</i>)-3-hydroxybutyrate [P(3HB)] is synthesized by a polymerizing enzyme called polyhydroxyalkanoate (PHA) synthase and accumulates in a wide variety of bacterial cells. Recently, we demonstrated the secretory production of a (<i>R</i>)-3...
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MDPI AG
2022-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/2/458 |
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| author | Saki Goto Yuki Miyahara Seiichi Taguchi Takeharu Tsuge Ayaka Hiroe |
| author_facet | Saki Goto Yuki Miyahara Seiichi Taguchi Takeharu Tsuge Ayaka Hiroe |
| author_sort | Saki Goto |
| collection | DOAJ |
| description | The biodegradable polyester poly-(<i>R</i>)-3-hydroxybutyrate [P(3HB)] is synthesized by a polymerizing enzyme called polyhydroxyalkanoate (PHA) synthase and accumulates in a wide variety of bacterial cells. Recently, we demonstrated the secretory production of a (<i>R</i>)-3HB oligomer (3HBO), a low-molecular-weight P(3HB), by using recombinant <i>Escherichia coli</i> expressing PHA synthases. The 3HBO has potential value as an antibacterial substance and as a building block for various polymers. In this study, to construct an efficient 3HBO production system, the coexpression of molecular chaperones and a PHA synthase derived from <i>Bacillus cereus</i> YB-4 (PhaRC<sub>YB4</sub>) was examined. First, genes encoding enzymes related to 3HBO biosynthesis (<i>phaRC<sub>YB4</sub></i>, <i>phaA</i> and <i>phaB</i> derived from <i>Ralstonia eutropha</i> H16) and two types of molecular chaperones (<i>groEL</i>, <i>groES</i>, and <i>tig</i>) were introduced into the <i>E. coli</i> strains BW25113 and BW25113Δ<i>adhE</i>. As a result, coexpression of the chaperones promoted the enzyme activity of PHA synthase (approximately 2–3-fold) and 3HBO production (approximately 2-fold). The expression assay of each chaperone and PHA synthase subunit (PhaR<sub>YB4</sub> and PhaC<sub>YB4</sub>) indicated that the combination of the two chaperone systems (GroEL-GroES and TF) supported the folding of PhaR<sub>YB4</sub> and PhaC<sub>YB4</sub>. These results suggest that the utilization of chaperone proteins is a valuable approach to enhance the formation of active PHA synthase and the productivity of 3HBO. |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T21:22:59Z |
| publishDate | 2022-02-01 |
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| series | Microorganisms |
| spelling | doaj.art-af58d98467ac4f99b55c260be184c5872023-11-23T21:16:32ZengMDPI AGMicroorganisms2076-26072022-02-0110245810.3390/microorganisms10020458Enhanced Production of (<i>R</i>)-3-Hydroxybutyrate Oligomers by Coexpression of Molecular Chaperones in Recombinant <i>Escherichia coli</i> Harboring a Polyhydroxyalkanoate Synthase Derived from <i>Bacillus cereus</i> YB-4Saki Goto0Yuki Miyahara1Seiichi Taguchi2Takeharu Tsuge3Ayaka Hiroe4Department of Chemistry for Life Sciences and Agriculture, Faculty of Life Sciences, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, JapanMIRAI, Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, JapanDepartment of Chemistry for Life Sciences and Agriculture, Faculty of Life Sciences, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, JapanMIRAI, Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, JapanDepartment of Chemistry for Life Sciences and Agriculture, Faculty of Life Sciences, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, JapanThe biodegradable polyester poly-(<i>R</i>)-3-hydroxybutyrate [P(3HB)] is synthesized by a polymerizing enzyme called polyhydroxyalkanoate (PHA) synthase and accumulates in a wide variety of bacterial cells. Recently, we demonstrated the secretory production of a (<i>R</i>)-3HB oligomer (3HBO), a low-molecular-weight P(3HB), by using recombinant <i>Escherichia coli</i> expressing PHA synthases. The 3HBO has potential value as an antibacterial substance and as a building block for various polymers. In this study, to construct an efficient 3HBO production system, the coexpression of molecular chaperones and a PHA synthase derived from <i>Bacillus cereus</i> YB-4 (PhaRC<sub>YB4</sub>) was examined. First, genes encoding enzymes related to 3HBO biosynthesis (<i>phaRC<sub>YB4</sub></i>, <i>phaA</i> and <i>phaB</i> derived from <i>Ralstonia eutropha</i> H16) and two types of molecular chaperones (<i>groEL</i>, <i>groES</i>, and <i>tig</i>) were introduced into the <i>E. coli</i> strains BW25113 and BW25113Δ<i>adhE</i>. As a result, coexpression of the chaperones promoted the enzyme activity of PHA synthase (approximately 2–3-fold) and 3HBO production (approximately 2-fold). The expression assay of each chaperone and PHA synthase subunit (PhaR<sub>YB4</sub> and PhaC<sub>YB4</sub>) indicated that the combination of the two chaperone systems (GroEL-GroES and TF) supported the folding of PhaR<sub>YB4</sub> and PhaC<sub>YB4</sub>. These results suggest that the utilization of chaperone proteins is a valuable approach to enhance the formation of active PHA synthase and the productivity of 3HBO.https://www.mdpi.com/2076-2607/10/2/458oligomerpolyhydroxyalkanoate (PHA)PHA synthasesecretory productionchaperones |
| spellingShingle | Saki Goto Yuki Miyahara Seiichi Taguchi Takeharu Tsuge Ayaka Hiroe Enhanced Production of (<i>R</i>)-3-Hydroxybutyrate Oligomers by Coexpression of Molecular Chaperones in Recombinant <i>Escherichia coli</i> Harboring a Polyhydroxyalkanoate Synthase Derived from <i>Bacillus cereus</i> YB-4 Microorganisms oligomer polyhydroxyalkanoate (PHA) PHA synthase secretory production chaperones |
| title | Enhanced Production of (<i>R</i>)-3-Hydroxybutyrate Oligomers by Coexpression of Molecular Chaperones in Recombinant <i>Escherichia coli</i> Harboring a Polyhydroxyalkanoate Synthase Derived from <i>Bacillus cereus</i> YB-4 |
| title_full | Enhanced Production of (<i>R</i>)-3-Hydroxybutyrate Oligomers by Coexpression of Molecular Chaperones in Recombinant <i>Escherichia coli</i> Harboring a Polyhydroxyalkanoate Synthase Derived from <i>Bacillus cereus</i> YB-4 |
| title_fullStr | Enhanced Production of (<i>R</i>)-3-Hydroxybutyrate Oligomers by Coexpression of Molecular Chaperones in Recombinant <i>Escherichia coli</i> Harboring a Polyhydroxyalkanoate Synthase Derived from <i>Bacillus cereus</i> YB-4 |
| title_full_unstemmed | Enhanced Production of (<i>R</i>)-3-Hydroxybutyrate Oligomers by Coexpression of Molecular Chaperones in Recombinant <i>Escherichia coli</i> Harboring a Polyhydroxyalkanoate Synthase Derived from <i>Bacillus cereus</i> YB-4 |
| title_short | Enhanced Production of (<i>R</i>)-3-Hydroxybutyrate Oligomers by Coexpression of Molecular Chaperones in Recombinant <i>Escherichia coli</i> Harboring a Polyhydroxyalkanoate Synthase Derived from <i>Bacillus cereus</i> YB-4 |
| title_sort | enhanced production of i r i 3 hydroxybutyrate oligomers by coexpression of molecular chaperones in recombinant i escherichia coli i harboring a polyhydroxyalkanoate synthase derived from i bacillus cereus i yb 4 |
| topic | oligomer polyhydroxyalkanoate (PHA) PHA synthase secretory production chaperones |
| url | https://www.mdpi.com/2076-2607/10/2/458 |
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