In vitro conservation of Valeriana officinalis L. through minimal growth
Minimal growth storage could be utilized for in vitro plant conservation. Modifying the culture medium or the environmental condition can reduce the plant growth. The aim of the experiment is to observe the effect of macronutrients on the reduction of valerian culture This research was conducted at...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2023-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/10/e3sconf_iseprolocal2023_03025.pdf |
| _version_ | 1797866520522522624 |
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| author | Syahid Sitti Fatimah Palindung Lusia Seti |
| author_facet | Syahid Sitti Fatimah Palindung Lusia Seti |
| author_sort | Syahid Sitti Fatimah |
| collection | DOAJ |
| description | Minimal growth storage could be utilized for in vitro plant conservation. Modifying the culture medium or the environmental condition can reduce the plant growth. The aim of the experiment is to observe the effect of macronutrients on the reduction of valerian culture This research was conducted at Tissue Culture Laboratory at the Indonesian Crops and Medicinal Research Institute (ISMCRI). Single axillary buds with leaves of valerian were used as explants. The treatment applied were :1) full-MS medium + 0.1 mg l-1 BAP, 2) ¾ MS + 0.1 mg l-1 BAP, 3) ½ MS + 0.1 mg l-1 BAP and, 4) ¼ MS + 0.1 mg l-1 BAP. After 12 weeks of conservation, the cultures were transferred into a plant growth regulator free-MS medium to observe their regeneration ability after the conservation. Then, the cultures were acclimatized at the greenhouse to observe their further growth. The result showed that using ¼ MS + 0.1 mg l-1 BAP was the best treatment to reduce the growth during 12 weeks of conservation. After in vitro storage, the cultures can grow normally in full strength plant growth regulator free-MS medium, which was characterized by the formation of new shoots. Valerian also produced roots in the same medium. All acclimatized plantlets were successfully established at the greenhouse. |
| first_indexed | 2024-04-09T23:26:30Z |
| format | Article |
| id | doaj.art-af5c392c8f944cb094bb0a145e5fbb5a |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-04-09T23:26:30Z |
| publishDate | 2023-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-af5c392c8f944cb094bb0a145e5fbb5a2023-03-21T10:31:46ZengEDP SciencesE3S Web of Conferences2267-12422023-01-013730302510.1051/e3sconf/202337303025e3sconf_iseprolocal2023_03025In vitro conservation of Valeriana officinalis L. through minimal growthSyahid Sitti Fatimah0Palindung Lusia Seti1Research Center for Horticultural and Estate Crops, National Research and Innovation AgencyResearch Center for Food Technology and Processing, National Research and Innovation AgencyMinimal growth storage could be utilized for in vitro plant conservation. Modifying the culture medium or the environmental condition can reduce the plant growth. The aim of the experiment is to observe the effect of macronutrients on the reduction of valerian culture This research was conducted at Tissue Culture Laboratory at the Indonesian Crops and Medicinal Research Institute (ISMCRI). Single axillary buds with leaves of valerian were used as explants. The treatment applied were :1) full-MS medium + 0.1 mg l-1 BAP, 2) ¾ MS + 0.1 mg l-1 BAP, 3) ½ MS + 0.1 mg l-1 BAP and, 4) ¼ MS + 0.1 mg l-1 BAP. After 12 weeks of conservation, the cultures were transferred into a plant growth regulator free-MS medium to observe their regeneration ability after the conservation. Then, the cultures were acclimatized at the greenhouse to observe their further growth. The result showed that using ¼ MS + 0.1 mg l-1 BAP was the best treatment to reduce the growth during 12 weeks of conservation. After in vitro storage, the cultures can grow normally in full strength plant growth regulator free-MS medium, which was characterized by the formation of new shoots. Valerian also produced roots in the same medium. All acclimatized plantlets were successfully established at the greenhouse.https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/10/e3sconf_iseprolocal2023_03025.pdf |
| spellingShingle | Syahid Sitti Fatimah Palindung Lusia Seti In vitro conservation of Valeriana officinalis L. through minimal growth E3S Web of Conferences |
| title | In vitro conservation of Valeriana officinalis L. through minimal growth |
| title_full | In vitro conservation of Valeriana officinalis L. through minimal growth |
| title_fullStr | In vitro conservation of Valeriana officinalis L. through minimal growth |
| title_full_unstemmed | In vitro conservation of Valeriana officinalis L. through minimal growth |
| title_short | In vitro conservation of Valeriana officinalis L. through minimal growth |
| title_sort | in vitro conservation of valeriana officinalis l through minimal growth |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/10/e3sconf_iseprolocal2023_03025.pdf |
| work_keys_str_mv | AT syahidsittifatimah invitroconservationofvalerianaofficinalislthroughminimalgrowth AT palindunglusiaseti invitroconservationofvalerianaofficinalislthroughminimalgrowth |