Comparative Genomic Analysis of a Thermophilic Protease-Producing Strain <i>Geobacillus stearothermophilus</i> H6
The genus <i>Geobacillus</i> comprises thermophilic gram-positive bacteria which are widely distributed, and their ability to withstand high temperatures makes them suitable for various applications in biotechnology and industrial production. <i>Geobacillus stearothermophilus</i...
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MDPI AG
2023-02-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/2/466 |
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| author | Ruilin Lai Min Lin Yongliang Yan Shijie Jiang Zhengfu Zhou Jin Wang |
| author_facet | Ruilin Lai Min Lin Yongliang Yan Shijie Jiang Zhengfu Zhou Jin Wang |
| author_sort | Ruilin Lai |
| collection | DOAJ |
| description | The genus <i>Geobacillus</i> comprises thermophilic gram-positive bacteria which are widely distributed, and their ability to withstand high temperatures makes them suitable for various applications in biotechnology and industrial production. <i>Geobacillus stearothermophilus</i> H6 is an extremely thermophilic <i>Geobacillus</i> strain isolated from hyperthermophilic compost at 80 °C. Through whole-genome sequencing and genome annotation analysis of the strain, the gene functions of <i>G. stearothermophilus</i> H6 were predicted and the thermophilic enzyme in the strain was mined. The <i>G. stearothermophilus</i> H6 draft genome consisted of 3,054,993 bp, with a genome GC content of 51.66%, and it was predicted to contain 3750 coding genes. The analysis showed that strain H6 contained a variety of enzyme-coding genes, including protease, glycoside hydrolase, xylanase, amylase and lipase genes. A skimmed milk plate experiment showed that <i>G. stearothermophilus</i> H6 could produce extracellular protease that functioned at 60 °C, and the genome predictions included 18 secreted proteases with signal peptides. By analyzing the sequence of the strain genome, a protease gene <i>gs-sp1</i> was successfully screened. The gene sequence was analyzed and heterologously expressed, and the protease was successfully expressed in <i>Escherichia coli</i>. These results could provide a theoretical basis for the development and application of industrial strains. |
| first_indexed | 2024-03-11T08:47:11Z |
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| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T08:47:11Z |
| publishDate | 2023-02-01 |
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| series | Genes |
| spelling | doaj.art-af5c661c6b0648c384db72ce5568455d2023-11-16T20:43:21ZengMDPI AGGenes2073-44252023-02-0114246610.3390/genes14020466Comparative Genomic Analysis of a Thermophilic Protease-Producing Strain <i>Geobacillus stearothermophilus</i> H6Ruilin Lai0Min Lin1Yongliang Yan2Shijie Jiang3Zhengfu Zhou4Jin Wang5College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621000, ChinaCollege of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621000, ChinaKey Laboratory of Agricultural Microbiome (MARA), Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaCollege of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621000, ChinaKey Laboratory of Agricultural Microbiome (MARA), Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaCollege of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621000, ChinaThe genus <i>Geobacillus</i> comprises thermophilic gram-positive bacteria which are widely distributed, and their ability to withstand high temperatures makes them suitable for various applications in biotechnology and industrial production. <i>Geobacillus stearothermophilus</i> H6 is an extremely thermophilic <i>Geobacillus</i> strain isolated from hyperthermophilic compost at 80 °C. Through whole-genome sequencing and genome annotation analysis of the strain, the gene functions of <i>G. stearothermophilus</i> H6 were predicted and the thermophilic enzyme in the strain was mined. The <i>G. stearothermophilus</i> H6 draft genome consisted of 3,054,993 bp, with a genome GC content of 51.66%, and it was predicted to contain 3750 coding genes. The analysis showed that strain H6 contained a variety of enzyme-coding genes, including protease, glycoside hydrolase, xylanase, amylase and lipase genes. A skimmed milk plate experiment showed that <i>G. stearothermophilus</i> H6 could produce extracellular protease that functioned at 60 °C, and the genome predictions included 18 secreted proteases with signal peptides. By analyzing the sequence of the strain genome, a protease gene <i>gs-sp1</i> was successfully screened. The gene sequence was analyzed and heterologously expressed, and the protease was successfully expressed in <i>Escherichia coli</i>. These results could provide a theoretical basis for the development and application of industrial strains.https://www.mdpi.com/2073-4425/14/2/466comparative genomicsgenome sequencing<i>G. stearothermophilus</i> H6thermostabilityprotease |
| spellingShingle | Ruilin Lai Min Lin Yongliang Yan Shijie Jiang Zhengfu Zhou Jin Wang Comparative Genomic Analysis of a Thermophilic Protease-Producing Strain <i>Geobacillus stearothermophilus</i> H6 Genes comparative genomics genome sequencing <i>G. stearothermophilus</i> H6 thermostability protease |
| title | Comparative Genomic Analysis of a Thermophilic Protease-Producing Strain <i>Geobacillus stearothermophilus</i> H6 |
| title_full | Comparative Genomic Analysis of a Thermophilic Protease-Producing Strain <i>Geobacillus stearothermophilus</i> H6 |
| title_fullStr | Comparative Genomic Analysis of a Thermophilic Protease-Producing Strain <i>Geobacillus stearothermophilus</i> H6 |
| title_full_unstemmed | Comparative Genomic Analysis of a Thermophilic Protease-Producing Strain <i>Geobacillus stearothermophilus</i> H6 |
| title_short | Comparative Genomic Analysis of a Thermophilic Protease-Producing Strain <i>Geobacillus stearothermophilus</i> H6 |
| title_sort | comparative genomic analysis of a thermophilic protease producing strain i geobacillus stearothermophilus i h6 |
| topic | comparative genomics genome sequencing <i>G. stearothermophilus</i> H6 thermostability protease |
| url | https://www.mdpi.com/2073-4425/14/2/466 |
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