Bacterial RuBisCO is required for efficient Bradyrhizobium/Aeschynomene symbiosis.
Rhizobia and legume plants establish symbiotic associations resulting in the formation of organs specialized in nitrogen fixation. In such organs, termed nodules, bacteria differentiate into bacteroids which convert atmospheric nitrogen and supply the plant with organic nitrogen. As a counterpart, b...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3130060?pdf=render |
| _version_ | 1817999720009695232 |
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| author | Benjamin Gourion Nathanaël Delmotte Katia Bonaldi Nico Nouwen Julia A Vorholt Eric Giraud |
| author_facet | Benjamin Gourion Nathanaël Delmotte Katia Bonaldi Nico Nouwen Julia A Vorholt Eric Giraud |
| author_sort | Benjamin Gourion |
| collection | DOAJ |
| description | Rhizobia and legume plants establish symbiotic associations resulting in the formation of organs specialized in nitrogen fixation. In such organs, termed nodules, bacteria differentiate into bacteroids which convert atmospheric nitrogen and supply the plant with organic nitrogen. As a counterpart, bacteroids receive carbon substrates from the plant. This rather simple model of metabolite exchange underlies symbiosis but does not describe the complexity of bacteroids' central metabolism. A previous study using the tropical symbiotic model Aeschynomene indica/photosynthetic Bradyrhizobium sp. ORS278 suggested a role of the bacterial Calvin cycle during the symbiotic process. Herein we investigated the role of two RuBisCO gene clusters of Bradyrhizobium sp. ORS278 during symbiosis. Using gene reporter fusion strains, we showed that cbbL1 but not the paralogous cbbL2 is expressed during symbiosis. Congruently, CbbL1 was detected in bacteroids by proteome analysis. The importance of CbbL1 for symbiotic nitrogen fixation was proven by a reverse genetic approach. Interestingly, despite its symbiotic nitrogen fixation defect, the cbbL1 mutant was not affected in nitrogen fixation activity under free living state. This study demonstrates a critical role for bacterial RuBisCO during a rhizobia/legume symbiotic interaction. |
| first_indexed | 2024-04-14T03:12:48Z |
| format | Article |
| id | doaj.art-af5dde1bab53455a8d1d8e4cb4899abe |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T03:12:48Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-af5dde1bab53455a8d1d8e4cb4899abe2022-12-22T02:15:34ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0167e2190010.1371/journal.pone.0021900Bacterial RuBisCO is required for efficient Bradyrhizobium/Aeschynomene symbiosis.Benjamin GourionNathanaël DelmotteKatia BonaldiNico NouwenJulia A VorholtEric GiraudRhizobia and legume plants establish symbiotic associations resulting in the formation of organs specialized in nitrogen fixation. In such organs, termed nodules, bacteria differentiate into bacteroids which convert atmospheric nitrogen and supply the plant with organic nitrogen. As a counterpart, bacteroids receive carbon substrates from the plant. This rather simple model of metabolite exchange underlies symbiosis but does not describe the complexity of bacteroids' central metabolism. A previous study using the tropical symbiotic model Aeschynomene indica/photosynthetic Bradyrhizobium sp. ORS278 suggested a role of the bacterial Calvin cycle during the symbiotic process. Herein we investigated the role of two RuBisCO gene clusters of Bradyrhizobium sp. ORS278 during symbiosis. Using gene reporter fusion strains, we showed that cbbL1 but not the paralogous cbbL2 is expressed during symbiosis. Congruently, CbbL1 was detected in bacteroids by proteome analysis. The importance of CbbL1 for symbiotic nitrogen fixation was proven by a reverse genetic approach. Interestingly, despite its symbiotic nitrogen fixation defect, the cbbL1 mutant was not affected in nitrogen fixation activity under free living state. This study demonstrates a critical role for bacterial RuBisCO during a rhizobia/legume symbiotic interaction.http://europepmc.org/articles/PMC3130060?pdf=render |
| spellingShingle | Benjamin Gourion Nathanaël Delmotte Katia Bonaldi Nico Nouwen Julia A Vorholt Eric Giraud Bacterial RuBisCO is required for efficient Bradyrhizobium/Aeschynomene symbiosis. PLoS ONE |
| title | Bacterial RuBisCO is required for efficient Bradyrhizobium/Aeschynomene symbiosis. |
| title_full | Bacterial RuBisCO is required for efficient Bradyrhizobium/Aeschynomene symbiosis. |
| title_fullStr | Bacterial RuBisCO is required for efficient Bradyrhizobium/Aeschynomene symbiosis. |
| title_full_unstemmed | Bacterial RuBisCO is required for efficient Bradyrhizobium/Aeschynomene symbiosis. |
| title_short | Bacterial RuBisCO is required for efficient Bradyrhizobium/Aeschynomene symbiosis. |
| title_sort | bacterial rubisco is required for efficient bradyrhizobium aeschynomene symbiosis |
| url | http://europepmc.org/articles/PMC3130060?pdf=render |
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