Desorption electrospray ionization mass spectrometry reveals lipid metabolism of individual oocytes and embryos.
Alteration of maternal lipid metabolism early in development has been shown to trigger obesity, insulin resistance, type 2 diabetes and cardiovascular diseases later in life in humans and animal models. Here, we set out to determine (i) lipid composition dynamics in single oocytes and preimplantatio...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3779253?pdf=render |
| _version_ | 1818174289721950208 |
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| author | Andrés Felipe González-Serrano Valentina Pirro Christina R Ferreira Paolo Oliveri Livia S Eberlin Julia Heinzmann Andrea Lucas-Hahn Heiner Niemann Robert Graham Cooks |
| author_facet | Andrés Felipe González-Serrano Valentina Pirro Christina R Ferreira Paolo Oliveri Livia S Eberlin Julia Heinzmann Andrea Lucas-Hahn Heiner Niemann Robert Graham Cooks |
| author_sort | Andrés Felipe González-Serrano |
| collection | DOAJ |
| description | Alteration of maternal lipid metabolism early in development has been shown to trigger obesity, insulin resistance, type 2 diabetes and cardiovascular diseases later in life in humans and animal models. Here, we set out to determine (i) lipid composition dynamics in single oocytes and preimplantation embryos by high mass resolution desorption electrospray ionization mass spectrometry (DESI-MS), using the bovine species as biological model, (ii) the metabolically most relevant lipid compounds by multivariate data analysis and (iii) lipid upstream metabolism by quantitative real-time PCR (qRT-PCR) analysis of several target genes (ACAT1, CPT 1b, FASN, SREBP1 and SCAP). Bovine oocytes and blastocysts were individually analyzed by DESI-MS in both positive and negative ion modes, without lipid extraction and under ambient conditions, and were profiled for free fatty acids (FFA), phospholipids (PL), cholesterol-related molecules, and triacylglycerols (TAG). Principal component analysis (PCA) and linear discriminant analysis (LDA), performed for the first time on DESI-MS fused data, allowed unequivocal discrimination between oocytes and blastocysts based on specific lipid profiles. This analytical approach resulted in broad and detailed lipid annotation of single oocytes and blastocysts. Results of DESI-MS and transcript regulation analysis demonstrate that blastocysts produced in vitro and their in vivo counterparts differed significantly in the homeostasis of cholesterol and FFA metabolism. These results should assist in the production of viable and healthy embryos by elucidating in vivo embryonic lipid metabolism. |
| first_indexed | 2024-12-11T19:42:02Z |
| format | Article |
| id | doaj.art-af5e118671d54bac915538feeeeddd4c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T19:42:02Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-af5e118671d54bac915538feeeeddd4c2022-12-22T00:52:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0189e7498110.1371/journal.pone.0074981Desorption electrospray ionization mass spectrometry reveals lipid metabolism of individual oocytes and embryos.Andrés Felipe González-SerranoValentina PirroChristina R FerreiraPaolo OliveriLivia S EberlinJulia HeinzmannAndrea Lucas-HahnHeiner NiemannRobert Graham CooksAlteration of maternal lipid metabolism early in development has been shown to trigger obesity, insulin resistance, type 2 diabetes and cardiovascular diseases later in life in humans and animal models. Here, we set out to determine (i) lipid composition dynamics in single oocytes and preimplantation embryos by high mass resolution desorption electrospray ionization mass spectrometry (DESI-MS), using the bovine species as biological model, (ii) the metabolically most relevant lipid compounds by multivariate data analysis and (iii) lipid upstream metabolism by quantitative real-time PCR (qRT-PCR) analysis of several target genes (ACAT1, CPT 1b, FASN, SREBP1 and SCAP). Bovine oocytes and blastocysts were individually analyzed by DESI-MS in both positive and negative ion modes, without lipid extraction and under ambient conditions, and were profiled for free fatty acids (FFA), phospholipids (PL), cholesterol-related molecules, and triacylglycerols (TAG). Principal component analysis (PCA) and linear discriminant analysis (LDA), performed for the first time on DESI-MS fused data, allowed unequivocal discrimination between oocytes and blastocysts based on specific lipid profiles. This analytical approach resulted in broad and detailed lipid annotation of single oocytes and blastocysts. Results of DESI-MS and transcript regulation analysis demonstrate that blastocysts produced in vitro and their in vivo counterparts differed significantly in the homeostasis of cholesterol and FFA metabolism. These results should assist in the production of viable and healthy embryos by elucidating in vivo embryonic lipid metabolism.http://europepmc.org/articles/PMC3779253?pdf=render |
| spellingShingle | Andrés Felipe González-Serrano Valentina Pirro Christina R Ferreira Paolo Oliveri Livia S Eberlin Julia Heinzmann Andrea Lucas-Hahn Heiner Niemann Robert Graham Cooks Desorption electrospray ionization mass spectrometry reveals lipid metabolism of individual oocytes and embryos. PLoS ONE |
| title | Desorption electrospray ionization mass spectrometry reveals lipid metabolism of individual oocytes and embryos. |
| title_full | Desorption electrospray ionization mass spectrometry reveals lipid metabolism of individual oocytes and embryos. |
| title_fullStr | Desorption electrospray ionization mass spectrometry reveals lipid metabolism of individual oocytes and embryos. |
| title_full_unstemmed | Desorption electrospray ionization mass spectrometry reveals lipid metabolism of individual oocytes and embryos. |
| title_short | Desorption electrospray ionization mass spectrometry reveals lipid metabolism of individual oocytes and embryos. |
| title_sort | desorption electrospray ionization mass spectrometry reveals lipid metabolism of individual oocytes and embryos |
| url | http://europepmc.org/articles/PMC3779253?pdf=render |
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