Protocol for ex vivo time lapse imaging of Drosophila melanogaster cytonemes
Summary: This protocol describes how to image time and spatially resolved time lapses of Drosophila air sac primordium (ASP) cytonemes in ex vivo cultures of wing imaginal discs. It describes how to manually measure the length of cytonemes using custom-made FIJI/ImageJ tools, and to analyze data usi...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722000181 |
| _version_ | 1818321075314884608 |
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| author | Guilherme O. Barbosa Thomas B. Kornberg |
| author_facet | Guilherme O. Barbosa Thomas B. Kornberg |
| author_sort | Guilherme O. Barbosa |
| collection | DOAJ |
| description | Summary: This protocol describes how to image time and spatially resolved time lapses of Drosophila air sac primordium (ASP) cytonemes in ex vivo cultures of wing imaginal discs. It describes how to manually measure the length of cytonemes using custom-made FIJI/ImageJ tools, and to analyze data using R/R-Studios pipeline. It can also be used for studies of cell division, organelle localization, and protein trafficking as well as other cellular materials that can be fluorescently tagged and imaged with minimal phototoxicity. |
| first_indexed | 2024-12-13T10:35:08Z |
| format | Article |
| id | doaj.art-af61ddfa51ec4e52a4eee157e6593238 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-13T10:35:08Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-af61ddfa51ec4e52a4eee157e65932382022-12-21T23:50:45ZengElsevierSTAR Protocols2666-16672022-03-0131101138Protocol for ex vivo time lapse imaging of Drosophila melanogaster cytonemesGuilherme O. Barbosa0Thomas B. Kornberg1Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA, USA; Corresponding authorCardiovascular Research Institute, University of California, San Francisco, San Francisco, CA, USA; Corresponding authorSummary: This protocol describes how to image time and spatially resolved time lapses of Drosophila air sac primordium (ASP) cytonemes in ex vivo cultures of wing imaginal discs. It describes how to manually measure the length of cytonemes using custom-made FIJI/ImageJ tools, and to analyze data using R/R-Studios pipeline. It can also be used for studies of cell division, organelle localization, and protein trafficking as well as other cellular materials that can be fluorescently tagged and imaged with minimal phototoxicity.http://www.sciencedirect.com/science/article/pii/S2666166722000181Cell BiologyDevelopmental biologyMicroscopyModel Organisms |
| spellingShingle | Guilherme O. Barbosa Thomas B. Kornberg Protocol for ex vivo time lapse imaging of Drosophila melanogaster cytonemes STAR Protocols Cell Biology Developmental biology Microscopy Model Organisms |
| title | Protocol for ex vivo time lapse imaging of Drosophila melanogaster cytonemes |
| title_full | Protocol for ex vivo time lapse imaging of Drosophila melanogaster cytonemes |
| title_fullStr | Protocol for ex vivo time lapse imaging of Drosophila melanogaster cytonemes |
| title_full_unstemmed | Protocol for ex vivo time lapse imaging of Drosophila melanogaster cytonemes |
| title_short | Protocol for ex vivo time lapse imaging of Drosophila melanogaster cytonemes |
| title_sort | protocol for ex vivo time lapse imaging of drosophila melanogaster cytonemes |
| topic | Cell Biology Developmental biology Microscopy Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722000181 |
| work_keys_str_mv | AT guilhermeobarbosa protocolforexvivotimelapseimagingofdrosophilamelanogastercytonemes AT thomasbkornberg protocolforexvivotimelapseimagingofdrosophilamelanogastercytonemes |