Protocol for ex vivo time lapse imaging of Drosophila melanogaster cytonemes

Protocol for ex vivo time lapse imaging of Drosophila melanogaster cytonemes

Summary: This protocol describes how to image time and spatially resolved time lapses of Drosophila air sac primordium (ASP) cytonemes in ex vivo cultures of wing imaginal discs. It describes how to manually measure the length of cytonemes using custom-made FIJI/ImageJ tools, and to analyze data usi...

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Bibliographic Details
Main Authors:	Guilherme O. Barbosa, Thomas B. Kornberg
Format:	Article
Language:	English
Published:	Elsevier 2022-03-01
Series:	STAR Protocols
Subjects:	Cell Biology Developmental biology Microscopy Model Organisms
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166722000181

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