Antibacterial Action of Protein Fraction Isolated from <i>Rapana venosa</i> Hemolymph against <i>Escherichia coli</i> NBIMCC 8785
Natural products and especially those from marine organisms are being intensively explored as an alternative to synthetic antibiotics. However, the exact mechanisms of their action are not yet well understood. The molecular masses of components in the hemolymph fraction with MW 50–100 kDa from <i...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/17/1/68 |
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| author | Mihaela Kirilova Yana Topalova Lyudmila Velkova Aleksandar Dolashki Dimitar Kaynarov Elmira Daskalova Nellie Zheleva |
| author_facet | Mihaela Kirilova Yana Topalova Lyudmila Velkova Aleksandar Dolashki Dimitar Kaynarov Elmira Daskalova Nellie Zheleva |
| author_sort | Mihaela Kirilova |
| collection | DOAJ |
| description | Natural products and especially those from marine organisms are being intensively explored as an alternative to synthetic antibiotics. However, the exact mechanisms of their action are not yet well understood. The molecular masses of components in the hemolymph fraction with MW 50–100 kDa from <i>Rapana venosa</i> were determined using ImageQuant™ TL v8.2.0 software based on electrophoretic analysis. Mainly, three types of compounds with antibacterial potential were identified, namely proteins with MW at 50.230 kDa, 62.100 kDa and 93.088 kDa that were homologous to peroxidase-like protein, aplicyanin A and L-amino acid oxidase and functional units with MW 50 kDa from <i>R. venous</i> hemocyanin. Data for their antibacterial effect on <i>Escherichia coli</i> NBIMCC 8785 were obtained by CTC/DAPI-based fluorescent analysis (analysis based on the use of a functional fluorescence probe). The fluorescent analyses demonstrated that a 50% concentration of the fraction with MW 50–100 kDa was able to eliminate 99% of the live bacteria. The antimicrobial effect was detectable even at a 1% concentration of the active compounds. The bacteria in this case had reduced metabolic activity and a 24% decreased size. The fraction had superior action compared with another mollusc product—snail slime—which killed 60% of the <i>E. coli</i> NBIMCC 8785 cells at a 50% concentration and had no effect at a 1% concentration. The obtained results demonstrate the high potential of the fraction with MW 50–100 kDa from <i>R. venosa</i> to eliminate and suppress the development of <i>Escherichia coli</i> NBIMCC 8785 bacteria and could be applied as an appropriate component of therapeutics with the potential to replace antibiotics to avoid the development of antibiotic resistance. |
| first_indexed | 2024-03-08T10:38:43Z |
| format | Article |
| id | doaj.art-af6427a46d834a88a438832fe4763b4e |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-08T10:38:43Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-af6427a46d834a88a438832fe4763b4e2024-01-26T18:05:39ZengMDPI AGPharmaceuticals1424-82472024-01-011716810.3390/ph17010068Antibacterial Action of Protein Fraction Isolated from <i>Rapana venosa</i> Hemolymph against <i>Escherichia coli</i> NBIMCC 8785Mihaela Kirilova0Yana Topalova1Lyudmila Velkova2Aleksandar Dolashki3Dimitar Kaynarov4Elmira Daskalova5Nellie Zheleva6Faculty of Biology, Sofia University, 8 Dragan Tzankov blvd., 1164 Sofia, BulgariaFaculty of Biology, Sofia University, 8 Dragan Tzankov blvd., 1164 Sofia, BulgariaInstitute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. Georgi Bonchev str., bl. 9, 1113 Sofia, BulgariaInstitute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. Georgi Bonchev str., bl. 9, 1113 Sofia, BulgariaInstitute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. Georgi Bonchev str., bl. 9, 1113 Sofia, BulgariaFaculty of Biology, Sofia University, 8 Dragan Tzankov blvd., 1164 Sofia, BulgariaCenter of Competence “Clean Technologies for Sustainable Environment—Water, Waste, Energy for Circular Economy”, 1000 Sofia, BulgariaNatural products and especially those from marine organisms are being intensively explored as an alternative to synthetic antibiotics. However, the exact mechanisms of their action are not yet well understood. The molecular masses of components in the hemolymph fraction with MW 50–100 kDa from <i>Rapana venosa</i> were determined using ImageQuant™ TL v8.2.0 software based on electrophoretic analysis. Mainly, three types of compounds with antibacterial potential were identified, namely proteins with MW at 50.230 kDa, 62.100 kDa and 93.088 kDa that were homologous to peroxidase-like protein, aplicyanin A and L-amino acid oxidase and functional units with MW 50 kDa from <i>R. venous</i> hemocyanin. Data for their antibacterial effect on <i>Escherichia coli</i> NBIMCC 8785 were obtained by CTC/DAPI-based fluorescent analysis (analysis based on the use of a functional fluorescence probe). The fluorescent analyses demonstrated that a 50% concentration of the fraction with MW 50–100 kDa was able to eliminate 99% of the live bacteria. The antimicrobial effect was detectable even at a 1% concentration of the active compounds. The bacteria in this case had reduced metabolic activity and a 24% decreased size. The fraction had superior action compared with another mollusc product—snail slime—which killed 60% of the <i>E. coli</i> NBIMCC 8785 cells at a 50% concentration and had no effect at a 1% concentration. The obtained results demonstrate the high potential of the fraction with MW 50–100 kDa from <i>R. venosa</i> to eliminate and suppress the development of <i>Escherichia coli</i> NBIMCC 8785 bacteria and could be applied as an appropriate component of therapeutics with the potential to replace antibiotics to avoid the development of antibiotic resistance.https://www.mdpi.com/1424-8247/17/1/68<i>Rapana venosa</i> hemolymph<i>Escherichia coli</i> NBIMCC 8785antimicrobial effect |
| spellingShingle | Mihaela Kirilova Yana Topalova Lyudmila Velkova Aleksandar Dolashki Dimitar Kaynarov Elmira Daskalova Nellie Zheleva Antibacterial Action of Protein Fraction Isolated from <i>Rapana venosa</i> Hemolymph against <i>Escherichia coli</i> NBIMCC 8785 Pharmaceuticals <i>Rapana venosa</i> hemolymph <i>Escherichia coli</i> NBIMCC 8785 antimicrobial effect |
| title | Antibacterial Action of Protein Fraction Isolated from <i>Rapana venosa</i> Hemolymph against <i>Escherichia coli</i> NBIMCC 8785 |
| title_full | Antibacterial Action of Protein Fraction Isolated from <i>Rapana venosa</i> Hemolymph against <i>Escherichia coli</i> NBIMCC 8785 |
| title_fullStr | Antibacterial Action of Protein Fraction Isolated from <i>Rapana venosa</i> Hemolymph against <i>Escherichia coli</i> NBIMCC 8785 |
| title_full_unstemmed | Antibacterial Action of Protein Fraction Isolated from <i>Rapana venosa</i> Hemolymph against <i>Escherichia coli</i> NBIMCC 8785 |
| title_short | Antibacterial Action of Protein Fraction Isolated from <i>Rapana venosa</i> Hemolymph against <i>Escherichia coli</i> NBIMCC 8785 |
| title_sort | antibacterial action of protein fraction isolated from i rapana venosa i hemolymph against i escherichia coli i nbimcc 8785 |
| topic | <i>Rapana venosa</i> hemolymph <i>Escherichia coli</i> NBIMCC 8785 antimicrobial effect |
| url | https://www.mdpi.com/1424-8247/17/1/68 |
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