Hematopoietic stem cells contribute to lymphatic endothelium.
Although the lymphatic system arises as an extension of venous vessels in the embryo, little is known about the role of circulating progenitors in the maintenance or development of lymphatic endothelium. Here, we investigated whether hematopoietic stem cells (HSCs) have the potential to give rise to...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2008-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2583952?pdf=render |
| _version_ | 1811319605086388224 |
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| author | Shuguang Jiang Alexis S Bailey Devorah C Goldman John R Swain Melissa H Wong Philip R Streeter William H Fleming |
| author_facet | Shuguang Jiang Alexis S Bailey Devorah C Goldman John R Swain Melissa H Wong Philip R Streeter William H Fleming |
| author_sort | Shuguang Jiang |
| collection | DOAJ |
| description | Although the lymphatic system arises as an extension of venous vessels in the embryo, little is known about the role of circulating progenitors in the maintenance or development of lymphatic endothelium. Here, we investigated whether hematopoietic stem cells (HSCs) have the potential to give rise to lymphatic endothelial cells (LEC).Following the transfer of marked HSCs into irradiated recipients, donor-derived LEC that co-express the lymphatic endothelial markers Lyve-1 and VEGFR-3 were identified in several tissues. HSC-derived LEC persisted for more than 12 months and contributed to approximately 3-4% of lymphatic vessels. Donor-derived LECs were not detected in mice transplanted with common myeloid progenitors and granulocyte/macrophage progenitors, suggesting that myeloid lineage commitment is not a requisite step in HSC contribution to lymphatic endothelium. Analysis of parabiotic mice revealed direct evidence for the existence of functional, circulating lymphatic progenitors in the absence of acute injury. Furthermore, the transplantation of HSCs into Apc(Min/+) mice resulted in the incorporation of donor-derived LEC into the lymphatic vessels of spontaneously arising intestinal tumors.Our results indicate that HSCs can contribute to normal and tumor associated lymphatic endothelium. These findings suggest that the modification of HSCs may be a novel approach for targeting tumor metastasis and attenuating diseases of the lymphatic system. |
| first_indexed | 2024-04-13T12:45:54Z |
| format | Article |
| id | doaj.art-af681d1d04de44faab05276f48ea5b0e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T12:45:54Z |
| publishDate | 2008-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-af681d1d04de44faab05276f48ea5b0e2022-12-22T02:46:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-01311e381210.1371/journal.pone.0003812Hematopoietic stem cells contribute to lymphatic endothelium.Shuguang JiangAlexis S BaileyDevorah C GoldmanJohn R SwainMelissa H WongPhilip R StreeterWilliam H FlemingAlthough the lymphatic system arises as an extension of venous vessels in the embryo, little is known about the role of circulating progenitors in the maintenance or development of lymphatic endothelium. Here, we investigated whether hematopoietic stem cells (HSCs) have the potential to give rise to lymphatic endothelial cells (LEC).Following the transfer of marked HSCs into irradiated recipients, donor-derived LEC that co-express the lymphatic endothelial markers Lyve-1 and VEGFR-3 were identified in several tissues. HSC-derived LEC persisted for more than 12 months and contributed to approximately 3-4% of lymphatic vessels. Donor-derived LECs were not detected in mice transplanted with common myeloid progenitors and granulocyte/macrophage progenitors, suggesting that myeloid lineage commitment is not a requisite step in HSC contribution to lymphatic endothelium. Analysis of parabiotic mice revealed direct evidence for the existence of functional, circulating lymphatic progenitors in the absence of acute injury. Furthermore, the transplantation of HSCs into Apc(Min/+) mice resulted in the incorporation of donor-derived LEC into the lymphatic vessels of spontaneously arising intestinal tumors.Our results indicate that HSCs can contribute to normal and tumor associated lymphatic endothelium. These findings suggest that the modification of HSCs may be a novel approach for targeting tumor metastasis and attenuating diseases of the lymphatic system.http://europepmc.org/articles/PMC2583952?pdf=render |
| spellingShingle | Shuguang Jiang Alexis S Bailey Devorah C Goldman John R Swain Melissa H Wong Philip R Streeter William H Fleming Hematopoietic stem cells contribute to lymphatic endothelium. PLoS ONE |
| title | Hematopoietic stem cells contribute to lymphatic endothelium. |
| title_full | Hematopoietic stem cells contribute to lymphatic endothelium. |
| title_fullStr | Hematopoietic stem cells contribute to lymphatic endothelium. |
| title_full_unstemmed | Hematopoietic stem cells contribute to lymphatic endothelium. |
| title_short | Hematopoietic stem cells contribute to lymphatic endothelium. |
| title_sort | hematopoietic stem cells contribute to lymphatic endothelium |
| url | http://europepmc.org/articles/PMC2583952?pdf=render |
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