Surface Functionalization of Poly(<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) Membranes with RGD-Grafted Poly(2-oxazoline) for Periodontal Tissue Engineering
Bone tissue defects resulting from periodontal disease are often treated using guided tissue regeneration (GTR). The barrier membranes utilized here should prevent soft tissue infiltration into the bony defect and simultaneously support bone regeneration. In this study, we designed a degradable poly...
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MDPI AG
2022-01-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/13/1/4 |
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| author | Anna M. Tryba Małgorzata Krok-Borkowicz Michał Kula Natalia Piergies Mateusz Marzec Erik Wegener Justyna Frączyk Rainer Jordan Beata Kolesińska Dieter Scharnweber Czesława Paluszkiewicz Elżbieta Pamuła |
| author_facet | Anna M. Tryba Małgorzata Krok-Borkowicz Michał Kula Natalia Piergies Mateusz Marzec Erik Wegener Justyna Frączyk Rainer Jordan Beata Kolesińska Dieter Scharnweber Czesława Paluszkiewicz Elżbieta Pamuła |
| author_sort | Anna M. Tryba |
| collection | DOAJ |
| description | Bone tissue defects resulting from periodontal disease are often treated using guided tissue regeneration (GTR). The barrier membranes utilized here should prevent soft tissue infiltration into the bony defect and simultaneously support bone regeneration. In this study, we designed a degradable poly(<span style="font-variant: small-caps;">l</span>-lactide-<i>co</i>-glycolide) (PLGA) membrane that was surface-modified with cell adhesive arginine-glycine-aspartic acid (RGD) motifs. For a novel method of membrane manufacture, the RGD motifs were coupled with the non-ionic amphiphilic polymer poly(2-oxazoline) (POx). The RGD-containing membranes were then prepared by solvent casting of PLGA, POx coupled with RGD (POx_RGD), and poly(ethylene glycol) (PEG) solution in methylene chloride (DCM), followed by DCM evaporation and PEG leaching. Successful coupling of RGD to POx was confirmed spectroscopically by Raman, Fourier transform infrared in attenuated reflection mode (FTIR-ATR), and X-ray photoelectron (XPS) spectroscopy, while successful immobilization of POx_RGD on the membrane surface was confirmed by XPS and FTIR-ATR. The resulting membranes had an asymmetric microstructure, as shown by scanning electron microscopy (SEM), where the glass-cured surface was more porous and had a higher surface area then the air-cured surface. The higher porosity should support bone tissue regeneration, while the air-cured side is more suited to preventing soft tissue infiltration. The behavior of osteoblast-like cells on PLGA membranes modified with POx_RGD was compared to cell behavior on PLGA foil, non-modified PLGA membranes, or PLGA membranes modified only with POx. For this, MG-63 cells were cultured for 4, 24, and 96 h on the membranes and analyzed by metabolic activity tests, live/dead staining, and fluorescent staining of actin fibers. The results showed bone cell adhesion, proliferation, and viability to be the highest on membranes modified with POx_RGD, making them possible candidates for GTR applications in periodontology and in bone tissue engineering. |
| first_indexed | 2024-03-09T19:36:48Z |
| format | Article |
| id | doaj.art-af6927eb4cf94c7495ce1de289e5f206 |
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| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-09T19:36:48Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
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| series | Journal of Functional Biomaterials |
| spelling | doaj.art-af6927eb4cf94c7495ce1de289e5f2062023-11-24T01:53:16ZengMDPI AGJournal of Functional Biomaterials2079-49832022-01-01131410.3390/jfb13010004Surface Functionalization of Poly(<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) Membranes with RGD-Grafted Poly(2-oxazoline) for Periodontal Tissue EngineeringAnna M. Tryba0Małgorzata Krok-Borkowicz1Michał Kula2Natalia Piergies3Mateusz Marzec4Erik Wegener5Justyna Frączyk6Rainer Jordan7Beata Kolesińska8Dieter Scharnweber9Czesława Paluszkiewicz10Elżbieta Pamuła11Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Al. Mickiewicza 30, 30-059 Kraków, PolandDepartment of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Al. Mickiewicza 30, 30-059 Kraków, PolandDepartment of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Al. Mickiewicza 30, 30-059 Kraków, PolandInstitute of Nuclear Physics, Polish Academy of Sciences, ul. Radzikowskiego 152, 31-342 Kraków, PolandAcademic Centre for Materials and Nanotechnology, AGH University of Science and Technology, Al. Mickiewicza 30, 30-059 Kraków, PolandFaculty of Chemistry and Food Chemistry, Technische Universität Dresden, Mommsenstr, 401069 Dresden, GermanyFaculty of Chemistry, Institute of Organic Chemistry, Łódź University of Technology, ul. Żeromskiego 116, 90-924 Łódź, PolandFaculty of Chemistry and Food Chemistry, Technische Universität Dresden, Mommsenstr, 401069 Dresden, GermanyFaculty of Chemistry, Institute of Organic Chemistry, Łódź University of Technology, ul. Żeromskiego 116, 90-924 Łódź, PolandMax Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Str. 27, 01069 Dresden, GermanyInstitute of Nuclear Physics, Polish Academy of Sciences, ul. Radzikowskiego 152, 31-342 Kraków, PolandDepartment of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Al. Mickiewicza 30, 30-059 Kraków, PolandBone tissue defects resulting from periodontal disease are often treated using guided tissue regeneration (GTR). The barrier membranes utilized here should prevent soft tissue infiltration into the bony defect and simultaneously support bone regeneration. In this study, we designed a degradable poly(<span style="font-variant: small-caps;">l</span>-lactide-<i>co</i>-glycolide) (PLGA) membrane that was surface-modified with cell adhesive arginine-glycine-aspartic acid (RGD) motifs. For a novel method of membrane manufacture, the RGD motifs were coupled with the non-ionic amphiphilic polymer poly(2-oxazoline) (POx). The RGD-containing membranes were then prepared by solvent casting of PLGA, POx coupled with RGD (POx_RGD), and poly(ethylene glycol) (PEG) solution in methylene chloride (DCM), followed by DCM evaporation and PEG leaching. Successful coupling of RGD to POx was confirmed spectroscopically by Raman, Fourier transform infrared in attenuated reflection mode (FTIR-ATR), and X-ray photoelectron (XPS) spectroscopy, while successful immobilization of POx_RGD on the membrane surface was confirmed by XPS and FTIR-ATR. The resulting membranes had an asymmetric microstructure, as shown by scanning electron microscopy (SEM), where the glass-cured surface was more porous and had a higher surface area then the air-cured surface. The higher porosity should support bone tissue regeneration, while the air-cured side is more suited to preventing soft tissue infiltration. The behavior of osteoblast-like cells on PLGA membranes modified with POx_RGD was compared to cell behavior on PLGA foil, non-modified PLGA membranes, or PLGA membranes modified only with POx. For this, MG-63 cells were cultured for 4, 24, and 96 h on the membranes and analyzed by metabolic activity tests, live/dead staining, and fluorescent staining of actin fibers. The results showed bone cell adhesion, proliferation, and viability to be the highest on membranes modified with POx_RGD, making them possible candidates for GTR applications in periodontology and in bone tissue engineering.https://www.mdpi.com/2079-4983/13/1/4poly(<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide)poly(ethylene glycol)poly(2-oxazoline)RGD sequencesphase separationperiodontology |
| spellingShingle | Anna M. Tryba Małgorzata Krok-Borkowicz Michał Kula Natalia Piergies Mateusz Marzec Erik Wegener Justyna Frączyk Rainer Jordan Beata Kolesińska Dieter Scharnweber Czesława Paluszkiewicz Elżbieta Pamuła Surface Functionalization of Poly(<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) Membranes with RGD-Grafted Poly(2-oxazoline) for Periodontal Tissue Engineering Journal of Functional Biomaterials poly(<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) poly(ethylene glycol) poly(2-oxazoline) RGD sequences phase separation periodontology |
| title | Surface Functionalization of Poly(<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) Membranes with RGD-Grafted Poly(2-oxazoline) for Periodontal Tissue Engineering |
| title_full | Surface Functionalization of Poly(<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) Membranes with RGD-Grafted Poly(2-oxazoline) for Periodontal Tissue Engineering |
| title_fullStr | Surface Functionalization of Poly(<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) Membranes with RGD-Grafted Poly(2-oxazoline) for Periodontal Tissue Engineering |
| title_full_unstemmed | Surface Functionalization of Poly(<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) Membranes with RGD-Grafted Poly(2-oxazoline) for Periodontal Tissue Engineering |
| title_short | Surface Functionalization of Poly(<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) Membranes with RGD-Grafted Poly(2-oxazoline) for Periodontal Tissue Engineering |
| title_sort | surface functionalization of poly span style font variant small caps l span lactide i co i glycolide membranes with rgd grafted poly 2 oxazoline for periodontal tissue engineering |
| topic | poly(<span style="font-variant: small-caps">l</span>-lactide-<i>co</i>-glycolide) poly(ethylene glycol) poly(2-oxazoline) RGD sequences phase separation periodontology |
| url | https://www.mdpi.com/2079-4983/13/1/4 |
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