A Validated HPLC Method for Quantitation of Rosmarinic Acid in a Polyherbal Syrup
Herbal medicines play a significant role in global healthcare systems. In this investigation, “Monzej soda” syrup was prepared by decocting a mixture containing Lavandula angustifolia, Melissa officinalis, Echium amoenum, Cordia myxa, Glycyrrhiza glabra, Ziziphus jujuba, Foeniculum vulgare, Fumaria...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Iranian Society of Pharmacognosy
2020-04-01
|
| Series: | Research Journal of Pharmacognosy |
| Subjects: | |
| Online Access: | http://www.rjpharmacognosy.ir/article_100220_e12fc2538c2a6d7d14bb889016d4624d.pdf |
| _version_ | 1818174569985343488 |
|---|---|
| author | Sara Zakerin Homa Hajimehdipoor Seyed Alireza Mortazavi* Rasool Choopani Shirin Fahimi Masoumeh Sabetkasaei Fatemeh Tavakolifar |
| author_facet | Sara Zakerin Homa Hajimehdipoor Seyed Alireza Mortazavi* Rasool Choopani Shirin Fahimi Masoumeh Sabetkasaei Fatemeh Tavakolifar |
| author_sort | Sara Zakerin |
| collection | DOAJ |
| description | Herbal medicines play a significant role in global healthcare systems. In this investigation, “Monzej soda” syrup was prepared by decocting a mixture containing Lavandula angustifolia, Melissa officinalis, Echium amoenum, Cordia myxa, Glycyrrhiza glabra, Ziziphus jujuba, Foeniculum vulgare, Fumaria parviflora, Adiantum capillus-veneris and Alhagi spp. Manna. along with glycerin, sodium benzoate and potacium sorbate. Physicochemical characteristics of the syrup were examined. Moreover, an HPLC technique was designed for analysis of rosmarinic acid in the syrup using C18 column, isocratic H3PO4 0.085% as the mobile phase, flow rate of 1 ml/min in λmax 330 nm. The method was validated for selectivity, linearity, precision, accuracy, LOD and LOQ. The herbal syrup was brown color with special taste and flavor. Density, pH, viscosity, dry residue, total phenolics, rosmarinic acid content were found 1.085 g/ml, 5.56, 5.35 cP, 15.22±0.43 %, 194 mg/100ml and 47.5 mg/100 ml, respectively. The syrup was stable during laboratory stability tests. The HPLC method was valid according to selectivity, linearity (72-110 µg/ml, r2: 0.9995), intra-day and inter-day precisions (RSD%˂2), accuracy (103.38-106.47%), LOD 1.6 µg/ml and LOQ 4.9 µg/ml. The syrup is a good candidate for pharmaceutical companies after pharmacological and clinical tests. |
| first_indexed | 2024-12-11T19:46:30Z |
| format | Article |
| id | doaj.art-af71ce7ce66d4152b8fac1635f2b31c6 |
| institution | Directory Open Access Journal |
| issn | 2345-4458 2345-5977 |
| language | English |
| last_indexed | 2024-12-11T19:46:30Z |
| publishDate | 2020-04-01 |
| publisher | Iranian Society of Pharmacognosy |
| record_format | Article |
| series | Research Journal of Pharmacognosy |
| spelling | doaj.art-af71ce7ce66d4152b8fac1635f2b31c62022-12-22T00:52:53ZengIranian Society of PharmacognosyResearch Journal of Pharmacognosy2345-44582345-59772020-04-017251110.22127/rjp.2019.205478.1527100220A Validated HPLC Method for Quantitation of Rosmarinic Acid in a Polyherbal SyrupSara Zakerin0Homa Hajimehdipoor1Seyed Alireza Mortazavi*2Rasool Choopani3Shirin Fahimi4Masoumeh Sabetkasaei5Fatemeh Tavakolifar6Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Department of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Department of Traditional Medicine, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Herbal medicines play a significant role in global healthcare systems. In this investigation, “Monzej soda” syrup was prepared by decocting a mixture containing Lavandula angustifolia, Melissa officinalis, Echium amoenum, Cordia myxa, Glycyrrhiza glabra, Ziziphus jujuba, Foeniculum vulgare, Fumaria parviflora, Adiantum capillus-veneris and Alhagi spp. Manna. along with glycerin, sodium benzoate and potacium sorbate. Physicochemical characteristics of the syrup were examined. Moreover, an HPLC technique was designed for analysis of rosmarinic acid in the syrup using C18 column, isocratic H3PO4 0.085% as the mobile phase, flow rate of 1 ml/min in λmax 330 nm. The method was validated for selectivity, linearity, precision, accuracy, LOD and LOQ. The herbal syrup was brown color with special taste and flavor. Density, pH, viscosity, dry residue, total phenolics, rosmarinic acid content were found 1.085 g/ml, 5.56, 5.35 cP, 15.22±0.43 %, 194 mg/100ml and 47.5 mg/100 ml, respectively. The syrup was stable during laboratory stability tests. The HPLC method was valid according to selectivity, linearity (72-110 µg/ml, r2: 0.9995), intra-day and inter-day precisions (RSD%˂2), accuracy (103.38-106.47%), LOD 1.6 µg/ml and LOQ 4.9 µg/ml. The syrup is a good candidate for pharmaceutical companies after pharmacological and clinical tests.http://www.rjpharmacognosy.ir/article_100220_e12fc2538c2a6d7d14bb889016d4624d.pdfdepressionhplciranian traditional medicinerosmarinic acidvalidation |
| spellingShingle | Sara Zakerin Homa Hajimehdipoor Seyed Alireza Mortazavi* Rasool Choopani Shirin Fahimi Masoumeh Sabetkasaei Fatemeh Tavakolifar A Validated HPLC Method for Quantitation of Rosmarinic Acid in a Polyherbal Syrup Research Journal of Pharmacognosy depression hplc iranian traditional medicine rosmarinic acid validation |
| title | A Validated HPLC Method for Quantitation of Rosmarinic Acid in a Polyherbal Syrup |
| title_full | A Validated HPLC Method for Quantitation of Rosmarinic Acid in a Polyherbal Syrup |
| title_fullStr | A Validated HPLC Method for Quantitation of Rosmarinic Acid in a Polyherbal Syrup |
| title_full_unstemmed | A Validated HPLC Method for Quantitation of Rosmarinic Acid in a Polyherbal Syrup |
| title_short | A Validated HPLC Method for Quantitation of Rosmarinic Acid in a Polyherbal Syrup |
| title_sort | validated hplc method for quantitation of rosmarinic acid in a polyherbal syrup |
| topic | depression hplc iranian traditional medicine rosmarinic acid validation |
| url | http://www.rjpharmacognosy.ir/article_100220_e12fc2538c2a6d7d14bb889016d4624d.pdf |
| work_keys_str_mv | AT sarazakerin avalidatedhplcmethodforquantitationofrosmarinicacidinapolyherbalsyrup AT homahajimehdipoor avalidatedhplcmethodforquantitationofrosmarinicacidinapolyherbalsyrup AT seyedalirezamortazavi avalidatedhplcmethodforquantitationofrosmarinicacidinapolyherbalsyrup AT rasoolchoopani avalidatedhplcmethodforquantitationofrosmarinicacidinapolyherbalsyrup AT shirinfahimi avalidatedhplcmethodforquantitationofrosmarinicacidinapolyherbalsyrup AT masoumehsabetkasaei avalidatedhplcmethodforquantitationofrosmarinicacidinapolyherbalsyrup AT fatemehtavakolifar avalidatedhplcmethodforquantitationofrosmarinicacidinapolyherbalsyrup AT sarazakerin validatedhplcmethodforquantitationofrosmarinicacidinapolyherbalsyrup AT homahajimehdipoor validatedhplcmethodforquantitationofrosmarinicacidinapolyherbalsyrup AT seyedalirezamortazavi validatedhplcmethodforquantitationofrosmarinicacidinapolyherbalsyrup AT rasoolchoopani validatedhplcmethodforquantitationofrosmarinicacidinapolyherbalsyrup AT shirinfahimi validatedhplcmethodforquantitationofrosmarinicacidinapolyherbalsyrup AT masoumehsabetkasaei validatedhplcmethodforquantitationofrosmarinicacidinapolyherbalsyrup AT fatemehtavakolifar validatedhplcmethodforquantitationofrosmarinicacidinapolyherbalsyrup |