Rapid Construction of Complex Plant RNA Virus Infectious cDNA Clones for Agroinfection Using a Yeast-E. coli-Agrobacterium Shuttle Vector

Rapid Construction of Complex Plant RNA Virus Infectious cDNA Clones for Agroinfection Using a Yeast-E. coli-Agrobacterium Shuttle Vector

The availability of infectious full-length clone is indispensable for reverse genetics studies of virus biology, pathology and construction of viral vectors. However, for RNA viruses with large genome sizes or those exhibiting inherent cloning difficulties, procedure to generate biologically active...

Full description

Bibliographic Details
Main Authors:	Kai Sun, Danyang Zhao, Yong Liu, Changjun Huang, Wei Zhang, Zhenghe Li
Format:	Article
Language:	English
Published:	MDPI AG 2017-11-01
Series:	Viruses
Subjects:	infectious cDNA clone agroinfection yeast-E.coli-Agrobacterium shuttle vector yeast homologous recombination potyvirus potato virus Y rhabdovirus sonchus yellow net virus
Online Access:	https://www.mdpi.com/1999-4915/9/11/332

Similar Items

Generation of a Triple-Shuttling Vector and the Application in Plant Plus-Strand RNA Virus Infectious cDNA Clone Construction
by: Chenwei Feng, et al.
Published: (2023-03-01)

Significantly Improved Recovery of Recombinant Sonchus Yellow Net Rhabdovirus by Expressing the Negative-Strand Genomic RNA
by: Xiaonan Ma, et al.
Published: (2020-12-01)

Response of tomato cultivars to agroinfection with Beet curly top Iran virus
by: Farnaz Khoshnazar, et al.
Published: (2016-09-01)

Molecular Characterization of Tomato Yellow Leaf Curl Virus in Korea and the Construction of an Infectious Clone
by: Bong Choon Lee, et al.
Published: (2015-06-01)

One-Step Assembly of a Porcine Epidemic Diarrhea Virus Infectious cDNA Clone by Homologous Recombination in Yeast: Rapid Manipulation of Viral Genome With CRISPR/Cas9 Gene-Editing Technology
by: Yanyang Zhou, et al.
Published: (2022-02-01)

Construction and characterization of an infectious cDNA clone of potato virus S developed from selected populations that survived genetic bottlenecks
by: Xin Li, et al.
Published: (2019-02-01)

The Agrobacterium tumefaciens Ti Plasmid Virulence Gene virE2 Reduces Sri Lankan Cassava Mosaic Virus Infection in Transgenic Nicotiana benthamiana Plants
by: Thulasi Raveendrannair Resmi, et al.
Published: (2015-05-01)

Development of a Full-Length Infectious cDNA Clone of the Grapevine Berry Inner Necrosis Virus
by: Xudong Fan, et al.
Published: (2020-10-01)

Discovery of the Agrobacterium growth inhibition sequence in virus and its application to recombinant clone screening
by: Jinlong Yin, et al.
Published: (2019-07-01)

Molecular Detection of Potato virus Y using Universal Primers from Ardabil Province
by: Aysan Ghasemzadeh, et al.
Published: (2012-07-01)

Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil
by: Jefferson José da Silva Santos, et al.
Published: (2015-01-01)

Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil
by: Jefferson José da Silva Santos, et al.
Published: (2015-07-01)

Auxotrophy-Independent Plasmid Shuttle Vectors for Applications in Diverse Yeasts
by: Jeremy R. Smith, et al.
Published: (2024-02-01)

Nuclear proteome of virus-infected and healthy potato leaves
by: Minna-Liisa Rajamäki, et al.
Published: (2020-07-01)

Is the Glycoprotein Responsible for the Differences in Dispersal Rates between Lettuce Necrotic Yellows Virus Subgroups?
by: Eko Y. Prabowo, et al.
Published: (2022-07-01)

Development of heat tolerant strawberry (Fragaria x ananassa dush., cv. camarosa) plants through Agrobacterium-mediated transformation
by: Haddadi, Fatemeh
Published: (2012)

Construction and characterization of a high-quality cDNA library of Cymbidium faberi suitable for yeast one- and two-hybrid assays
by: Yanqin Xu, et al.
Published: (2020-01-01)

Synthesis and Characterization of a Full-Length Infectious cDNA Clone of <i>Tomato Mottle Mosaic Virus</i>
by: Liqin Tu, et al.
Published: (2021-06-01)

Nucleocytoplasmic Shuttling of Influenza A Virus Proteins
by: Jing Li, et al.
Published: (2015-05-01)

First characterization of infectious cDNA clones of <em>Olive mild mosaic virus</em>
by: Joana CARDOSO, et al.
Published: (2012-08-01)

Identification of a novel Getah virus by Virus-Discovery-cDNA random amplified polymorphic DNA (RAPD)
by: Hu Tingsong, et al.
Published: (2012-12-01)

Production of Potyvirus-Derived Nanoparticles Decorated with a Nanobody in Biofactory Plants
by: Maricarmen Martí, et al.
Published: (2022-03-01)

The relationship and relativity between three isolates of Potato virus Y Potyvirus infecting potato (Solanum tuberosum L.) at Alexandria and El-Beheira governorates, northern Egypt
by: Mahmoud H. Abd El-Aziz, et al.
Published: (2019-08-01)

ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINE
by: UTUT WIDYASTUT, et al.
Published: (2009-01-01)

The First Occurrence of Zucchini yellow mosaic virus Infecting Cucumber in Padang, West Sumatra
by: Muhammad Arif Rido, et al.
Published: (2023-10-01)

Interferon Response and Viral Evasion by Members of the Family Rhabdoviridae
by: Matthias J. Schnell, et al.
Published: (2009-11-01)

Development of Stable Infectious cDNA Clones of Tomato Black Ring Virus Tagged with Green Fluorescent Protein
by: Aleksandra Zarzyńska-Nowak, et al.
Published: (2024-01-01)

Molecular Characterization and Pathogenicity of an Infectious cDNA Clone of Youcai Mosaic Virus on <i>Solanum nigrum</i>
by: Tianxiao Gu, et al.
Published: (2024-01-01)

Effects of various parameters influencing the quality of results in a cDNA-AFLP technique used for transcriptom analysis of potato
by: Kobra Moslem Khani, et al.
Published: (2014-05-01)

In Vitro Synthesized RNA Generated from cDNA Clones of Both Genomic Components of Cucurbit yellow stunting disorder virus Replicates in Cucumber Protoplasts
by: Carolyn A. Owen, et al.
Published: (2016-06-01)

Molecular characteristics and pathogenicity of infectious cDNA clones of the Chinese isolates of pea enation mosaic virus 1 and pea enation mosaic virus 2
by: Xiaojiao Chen, et al.
Published: (2023-04-01)

The Additional 15 nt of 5′ UTR in a Novel Recombinant Isolate of Chilli Veinal Mottle Virus in <i>Solanum nigrum</i> L. Is Crucial for Infection
by: Qionglian Wan, et al.
Published: (2023-06-01)

Penyakit Bercak Kuning Ubi Jalar di Bogor, Jawa Barat
by: Anastasya Hondo, et al.
Published: (2018-10-01)

<it>Agrobacterium</it>-mediated transformation of rough lemon (<it>Citrus jambhiri</it> Lush) with yeast <it>HAL2</it> gene
by: Ali Shawkat, et al.
Published: (2012-06-01)

Lyssavirus Surveillance in Bats, Bangladesh
by: Ivan V. Kuzmin, et al.
Published: (2006-03-01)

Resistance to Rhabdoviridae Infection and Subversion of Antiviral Responses
by: Danielle Blondel, et al.
Published: (2015-07-01)

Isolation of cDNA Fragment of Gene Encoding for Actin from Melastoma malabthricum.
by: Suharsono, et al.
Published: (2010-11-01)

Screening of Tnfaip1-Interacting Proteins in Zebrafish Embryonic cDNA Libraries Using a Yeast Two-Hybrid System
by: Shulan Huang, et al.
Published: (2023-10-01)

Revised Taxonomy of Rhabdoviruses Infecting Fish and Marine Mammals
by: Peter J. Walker, et al.
Published: (2022-05-01)

Efficient translation of Eggplant mottled dwarf nucleorhabdovirus N and X genes requires both 5′ and 3′ UTRs
by: Ghobad Babaei, et al.
Published: (2021-06-01)