Anti-double stranded DNA antibodies: A rational diagnostic approach in limited-resource settings
Context: Anti-double-stranded deoxyribonucleic acid antibodies (dsDNA Abs) are highly specific markers of systemic lupus erythematosus (SLE). Multiple methods are employed for their detection in routine diagnostics. Objectives: The aim of this study was to evaluate a diagnostic approach for anti-dsD...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Practical Laboratory Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352551722000245 |
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| author | Brahim Admou Fatima-ezzohra Eddehbi Lahcen Elmoumou Saad Elmojadili Abdelmouïne Salami Mohammed Oujidi Imane Brahim Raja Hazime |
| author_facet | Brahim Admou Fatima-ezzohra Eddehbi Lahcen Elmoumou Saad Elmojadili Abdelmouïne Salami Mohammed Oujidi Imane Brahim Raja Hazime |
| author_sort | Brahim Admou |
| collection | DOAJ |
| description | Context: Anti-double-stranded deoxyribonucleic acid antibodies (dsDNA Abs) are highly specific markers of systemic lupus erythematosus (SLE). Multiple methods are employed for their detection in routine diagnostics. Objectives: The aim of this study was to evaluate a diagnostic approach for anti-dsDNA Abs using DNA-ELISA and Crithidia luciliae fluorescence test (CLIFT), in combination with antinuclear antibody (ANA) screening. Methods: We enrolled 113 patients—53 with SLE, 50 with other systemic autoimmune rheumatic diseases (OSARD), and 10 with non-autoimmune clinical conditions (NAICC).Patients’ samples were tested for anti-dsDNA Abs using an enzyme-linked immunosorbent assay (ELISA) and CLIFT, combined to ANA screening by indirect immunofluorescence assay (ANA-IIFA). Results: The mean age of patients was 39.94 ± 15 years (ranges: 11–85 years). Overall, specimens from 77.3%, 11.7%, and 20% of patients with SLE, OSARD and NAICC respectively were ELISA-positive; and those from 54.7% to 4% of patients with SLE and OSARD, respectively, were CLIFT-positive. CLIFT positivity was significantly associated with high ELISA titers (p = 0.002) and homogeneous ANA-IIF pattern (p = 0.0002). Conclusion: For better clinical relevance of anti-dsDNA antibodies, we suggest a combined detection strategy based on ELISA, CLIFT and ANA-IIFA, considering the clinical criteria of SLE. |
| first_indexed | 2024-12-10T22:10:00Z |
| format | Article |
| id | doaj.art-af7500edaefc49e6aa7fd6a940402f7d |
| institution | Directory Open Access Journal |
| issn | 2352-5517 |
| language | English |
| last_indexed | 2024-12-10T22:10:00Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Practical Laboratory Medicine |
| spelling | doaj.art-af7500edaefc49e6aa7fd6a940402f7d2022-12-22T01:31:38ZengElsevierPractical Laboratory Medicine2352-55172022-08-0131e00285Anti-double stranded DNA antibodies: A rational diagnostic approach in limited-resource settingsBrahim Admou0Fatima-ezzohra Eddehbi1Lahcen Elmoumou2Saad Elmojadili3Abdelmouïne Salami4Mohammed Oujidi5Imane Brahim6Raja Hazime7Laboratory of Immunology, University Hospital Mohamed VI, Marrakech, Morocco; Bioscience's Research Laboratory, Faculty of Medicine and Pharmacy, Cadi Ayyad University, Marrakech, Morocco; Center of Clinical Research, University Hospital Mohamed VI, Marrakech, Morocco; Corresponding author. Center of Clinical Research, University Hospital Mohamed VI, Marrakech, Morocco.Laboratory of Immunology, University Hospital Mohamed VI, Marrakech, MoroccoLaboratory of Immunology, University Hospital Mohamed VI, Marrakech, MoroccoLaboratory of Immunology, University Hospital Mohamed VI, Marrakech, MoroccoLaboratory of Immunology, University Hospital Mohamed VI, Marrakech, MoroccoLaboratory of Immunology, University Hospital Mohamed VI, Marrakech, MoroccoLaboratory of Immunology, University Hospital Mohamed VI, Marrakech, MoroccoLaboratory of Immunology, University Hospital Mohamed VI, Marrakech, Morocco; Bioscience's Research Laboratory, Faculty of Medicine and Pharmacy, Cadi Ayyad University, Marrakech, MoroccoContext: Anti-double-stranded deoxyribonucleic acid antibodies (dsDNA Abs) are highly specific markers of systemic lupus erythematosus (SLE). Multiple methods are employed for their detection in routine diagnostics. Objectives: The aim of this study was to evaluate a diagnostic approach for anti-dsDNA Abs using DNA-ELISA and Crithidia luciliae fluorescence test (CLIFT), in combination with antinuclear antibody (ANA) screening. Methods: We enrolled 113 patients—53 with SLE, 50 with other systemic autoimmune rheumatic diseases (OSARD), and 10 with non-autoimmune clinical conditions (NAICC).Patients’ samples were tested for anti-dsDNA Abs using an enzyme-linked immunosorbent assay (ELISA) and CLIFT, combined to ANA screening by indirect immunofluorescence assay (ANA-IIFA). Results: The mean age of patients was 39.94 ± 15 years (ranges: 11–85 years). Overall, specimens from 77.3%, 11.7%, and 20% of patients with SLE, OSARD and NAICC respectively were ELISA-positive; and those from 54.7% to 4% of patients with SLE and OSARD, respectively, were CLIFT-positive. CLIFT positivity was significantly associated with high ELISA titers (p = 0.002) and homogeneous ANA-IIF pattern (p = 0.0002). Conclusion: For better clinical relevance of anti-dsDNA antibodies, we suggest a combined detection strategy based on ELISA, CLIFT and ANA-IIFA, considering the clinical criteria of SLE.http://www.sciencedirect.com/science/article/pii/S2352551722000245Anti-ds DNA antibodiesELISACLIFTAntinuclear antibodiesDiagnostic approachLimited resource settings |
| spellingShingle | Brahim Admou Fatima-ezzohra Eddehbi Lahcen Elmoumou Saad Elmojadili Abdelmouïne Salami Mohammed Oujidi Imane Brahim Raja Hazime Anti-double stranded DNA antibodies: A rational diagnostic approach in limited-resource settings Practical Laboratory Medicine Anti-ds DNA antibodies ELISA CLIFT Antinuclear antibodies Diagnostic approach Limited resource settings |
| title | Anti-double stranded DNA antibodies: A rational diagnostic approach in limited-resource settings |
| title_full | Anti-double stranded DNA antibodies: A rational diagnostic approach in limited-resource settings |
| title_fullStr | Anti-double stranded DNA antibodies: A rational diagnostic approach in limited-resource settings |
| title_full_unstemmed | Anti-double stranded DNA antibodies: A rational diagnostic approach in limited-resource settings |
| title_short | Anti-double stranded DNA antibodies: A rational diagnostic approach in limited-resource settings |
| title_sort | anti double stranded dna antibodies a rational diagnostic approach in limited resource settings |
| topic | Anti-ds DNA antibodies ELISA CLIFT Antinuclear antibodies Diagnostic approach Limited resource settings |
| url | http://www.sciencedirect.com/science/article/pii/S2352551722000245 |
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