Enzyme-Assisted Release of Antioxidant Peptides from <i>Porphyra dioica</i> Conchocelis
The conchocelis life cycle stage of <i>P. dioica</i> represents an unexplored source of bioactive compounds. The aim of this study was to generate and characterise, for the first time, hydrolysates of conchocelis using a specific combination of proteases (Prolyve<sup>®</sup>...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/2/249 |
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| author | Filipa B. Pimentel Marlene Machado Maria Cermeño Thanyaporn Kleekayai Susana Machado Andreia M. Rego Maria H. Abreu Rita C. Alves Maria Beatriz P. P. Oliveira Richard J. FitzGerald |
| author_facet | Filipa B. Pimentel Marlene Machado Maria Cermeño Thanyaporn Kleekayai Susana Machado Andreia M. Rego Maria H. Abreu Rita C. Alves Maria Beatriz P. P. Oliveira Richard J. FitzGerald |
| author_sort | Filipa B. Pimentel |
| collection | DOAJ |
| description | The conchocelis life cycle stage of <i>P. dioica</i> represents an unexplored source of bioactive compounds. The aim of this study was to generate and characterise, for the first time, hydrolysates of conchocelis using a specific combination of proteases (Prolyve<sup>®</sup> and Flavourzyme<sup>®</sup>). Hydrolysate molecular mass distribution and free amino acid contents were assessed, and the antioxidant activity was determined using a range of in vitro assays. The protein content and the total amino acid profiles of conchocelis were also studied. Conchocelis contained ~25% of protein (dry weight basis) and had a complete profile of essential amino acids. Direct sequential enzymatic treatment modified the profile of the generated compounds, increasing the amount of low molecular weight peptides (<1 kDa). There was a significant improvement in the antioxidant activity of the hydrolysates compared with the control (up to 2.5-fold), indicating their potential as a novel source of antioxidant ingredients. |
| first_indexed | 2024-03-09T05:22:27Z |
| format | Article |
| id | doaj.art-af75fc533262490aa54d2cd7ad12d774 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T05:22:27Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-af75fc533262490aa54d2cd7ad12d7742023-12-03T12:39:36ZengMDPI AGAntioxidants2076-39212021-02-0110224910.3390/antiox10020249Enzyme-Assisted Release of Antioxidant Peptides from <i>Porphyra dioica</i> ConchocelisFilipa B. Pimentel0Marlene Machado1Maria Cermeño2Thanyaporn Kleekayai3Susana Machado4Andreia M. Rego5Maria H. Abreu6Rita C. Alves7Maria Beatriz P. P. Oliveira8Richard J. FitzGerald9LAQV, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalLAQV, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalProteins and Peptides Research Group, Department of Biological Sciences, University of Limerick, V94 T9PX Limerick, IrelandProteins and Peptides Research Group, Department of Biological Sciences, University of Limerick, V94 T9PX Limerick, IrelandLAQV, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalALGAplus—Produção e Comercialização de Algas e Seus Derivados, Lda., 3830-196 Ílhavo, PortugalALGAplus—Produção e Comercialização de Algas e Seus Derivados, Lda., 3830-196 Ílhavo, PortugalLAQV, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalLAQV, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalProteins and Peptides Research Group, Department of Biological Sciences, University of Limerick, V94 T9PX Limerick, IrelandThe conchocelis life cycle stage of <i>P. dioica</i> represents an unexplored source of bioactive compounds. The aim of this study was to generate and characterise, for the first time, hydrolysates of conchocelis using a specific combination of proteases (Prolyve<sup>®</sup> and Flavourzyme<sup>®</sup>). Hydrolysate molecular mass distribution and free amino acid contents were assessed, and the antioxidant activity was determined using a range of in vitro assays. The protein content and the total amino acid profiles of conchocelis were also studied. Conchocelis contained ~25% of protein (dry weight basis) and had a complete profile of essential amino acids. Direct sequential enzymatic treatment modified the profile of the generated compounds, increasing the amount of low molecular weight peptides (<1 kDa). There was a significant improvement in the antioxidant activity of the hydrolysates compared with the control (up to 2.5-fold), indicating their potential as a novel source of antioxidant ingredients.https://www.mdpi.com/2076-3921/10/2/249<i>Porphyra dioica</i> conchocelisamino acidsenzyme-assisted releasereactive oxygen speciesantioxidant activity |
| spellingShingle | Filipa B. Pimentel Marlene Machado Maria Cermeño Thanyaporn Kleekayai Susana Machado Andreia M. Rego Maria H. Abreu Rita C. Alves Maria Beatriz P. P. Oliveira Richard J. FitzGerald Enzyme-Assisted Release of Antioxidant Peptides from <i>Porphyra dioica</i> Conchocelis Antioxidants <i>Porphyra dioica</i> conchocelis amino acids enzyme-assisted release reactive oxygen species antioxidant activity |
| title | Enzyme-Assisted Release of Antioxidant Peptides from <i>Porphyra dioica</i> Conchocelis |
| title_full | Enzyme-Assisted Release of Antioxidant Peptides from <i>Porphyra dioica</i> Conchocelis |
| title_fullStr | Enzyme-Assisted Release of Antioxidant Peptides from <i>Porphyra dioica</i> Conchocelis |
| title_full_unstemmed | Enzyme-Assisted Release of Antioxidant Peptides from <i>Porphyra dioica</i> Conchocelis |
| title_short | Enzyme-Assisted Release of Antioxidant Peptides from <i>Porphyra dioica</i> Conchocelis |
| title_sort | enzyme assisted release of antioxidant peptides from i porphyra dioica i conchocelis |
| topic | <i>Porphyra dioica</i> conchocelis amino acids enzyme-assisted release reactive oxygen species antioxidant activity |
| url | https://www.mdpi.com/2076-3921/10/2/249 |
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