Aldosterone disrupts the intercellular flow of glucose in cardiac muscle.Implications for the failing and diabetic heart.
The activation of the renin angiotensin system is known to impairs intercellular communication in the heart but the role of aldosterone on the process of chemical communication and particularly the intercellular diffusion of glucose between cardiomyocytes, is not known.This problem was investigated...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-12-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fendo.2015.00185/full |
| _version_ | 1818890851240116224 |
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| author | Walmor eDe Mello |
| author_facet | Walmor eDe Mello |
| author_sort | Walmor eDe Mello |
| collection | DOAJ |
| description | The activation of the renin angiotensin system is known to impairs intercellular communication in the heart but the role of aldosterone on the process of chemical communication and particularly the intercellular diffusion of glucose between cardiomyocytes, is not known.This problem was investigated in cell pairs isolated from the left ventricle of adult Wistar Kyoto rats. For this, fluorescent glucose was dialyzed into one cell of the pair using the whole cell clamp technique, and its diffusion from cell-to-cell through gap junctions was followed by measuring the fluorescence intensity in the dialyzed as well as in non-dialyzed cell as a function of time. The results indicated that ;1) in cell pairs exposed to aldosterone (100nM) for 24 hours the intercellular flow of glucose through gap junctions was disrupted;2) although the mechanism by which aldosterone disrupts the cell-to-cell flow of glucose is multifactorial two major factors are involved: oxidative stress and PKC activation; 3) the effect of aldosterone was significantly reduced by spironolactone (100nM); 4) calculation of gap junction permeability (Pj) indicated an average values of 0.3 +/- 0.001x10-4 cm/s (n=31) (4 animals) for controls and 24 +/ 0.03 x10-6 cm/s; (n=34) (4 animals) (P<0.05) for cell pairs exposed to aldosterone (100nM) for 24 hours.Bis-1 (10-9M), which is a selective PKC inhibitor, added to the aldosterone solution, improved the value of Pj to 0.21 +/- 0.001x10-4 cms/s (n=24)(P<0.05) while spironolactone (100nM) added to aldosterone solution, reduced significantly the effect of the hormone on junctional permeability to glucose. |
| first_indexed | 2024-12-19T17:31:29Z |
| format | Article |
| id | doaj.art-af7a59ff74424430b5dcd5662f54a25f |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-12-19T17:31:29Z |
| publishDate | 2015-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-af7a59ff74424430b5dcd5662f54a25f2022-12-21T20:12:26ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922015-12-01610.3389/fendo.2015.00185169084Aldosterone disrupts the intercellular flow of glucose in cardiac muscle.Implications for the failing and diabetic heart.Walmor eDe Mello0School of Medicine Medical Sciences Campus,UPRThe activation of the renin angiotensin system is known to impairs intercellular communication in the heart but the role of aldosterone on the process of chemical communication and particularly the intercellular diffusion of glucose between cardiomyocytes, is not known.This problem was investigated in cell pairs isolated from the left ventricle of adult Wistar Kyoto rats. For this, fluorescent glucose was dialyzed into one cell of the pair using the whole cell clamp technique, and its diffusion from cell-to-cell through gap junctions was followed by measuring the fluorescence intensity in the dialyzed as well as in non-dialyzed cell as a function of time. The results indicated that ;1) in cell pairs exposed to aldosterone (100nM) for 24 hours the intercellular flow of glucose through gap junctions was disrupted;2) although the mechanism by which aldosterone disrupts the cell-to-cell flow of glucose is multifactorial two major factors are involved: oxidative stress and PKC activation; 3) the effect of aldosterone was significantly reduced by spironolactone (100nM); 4) calculation of gap junction permeability (Pj) indicated an average values of 0.3 +/- 0.001x10-4 cm/s (n=31) (4 animals) for controls and 24 +/ 0.03 x10-6 cm/s; (n=34) (4 animals) (P<0.05) for cell pairs exposed to aldosterone (100nM) for 24 hours.Bis-1 (10-9M), which is a selective PKC inhibitor, added to the aldosterone solution, improved the value of Pj to 0.21 +/- 0.001x10-4 cms/s (n=24)(P<0.05) while spironolactone (100nM) added to aldosterone solution, reduced significantly the effect of the hormone on junctional permeability to glucose.http://journal.frontiersin.org/Journal/10.3389/fendo.2015.00185/fullAldosteroneMusclesPermeabilityCardiacgap junction |
| spellingShingle | Walmor eDe Mello Aldosterone disrupts the intercellular flow of glucose in cardiac muscle.Implications for the failing and diabetic heart. Frontiers in Endocrinology Aldosterone Muscles Permeability Cardiac gap junction |
| title | Aldosterone disrupts the intercellular flow of glucose in cardiac muscle.Implications for the failing and diabetic heart. |
| title_full | Aldosterone disrupts the intercellular flow of glucose in cardiac muscle.Implications for the failing and diabetic heart. |
| title_fullStr | Aldosterone disrupts the intercellular flow of glucose in cardiac muscle.Implications for the failing and diabetic heart. |
| title_full_unstemmed | Aldosterone disrupts the intercellular flow of glucose in cardiac muscle.Implications for the failing and diabetic heart. |
| title_short | Aldosterone disrupts the intercellular flow of glucose in cardiac muscle.Implications for the failing and diabetic heart. |
| title_sort | aldosterone disrupts the intercellular flow of glucose in cardiac muscle implications for the failing and diabetic heart |
| topic | Aldosterone Muscles Permeability Cardiac gap junction |
| url | http://journal.frontiersin.org/Journal/10.3389/fendo.2015.00185/full |
| work_keys_str_mv | AT walmoredemello aldosteronedisruptstheintercellularflowofglucoseincardiacmuscleimplicationsforthefailinganddiabeticheart |