Morphological description, genetic diversity and population structure of safflower (Carthamus tinctorius L.) mini core collection using SRAP and SSR markers
This study was conducted to investigate the genetic diversity and population structure of globally diverse mini core collection of safflower (Carthamus tinctorius L.) using agro-morphological, sequence-related amplified polymorphism (SRAP) and simple-sequence repeats (SSR) molecular markers. Field e...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13102818.2020.1818620 |
| _version_ | 1819078088996159488 |
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| author | Seyed Mohammad Reza Hassani Reza Talebi Sayyed Saeid Pourdad Amir Mohammad Naji Farzad Fayaz |
| author_facet | Seyed Mohammad Reza Hassani Reza Talebi Sayyed Saeid Pourdad Amir Mohammad Naji Farzad Fayaz |
| author_sort | Seyed Mohammad Reza Hassani |
| collection | DOAJ |
| description | This study was conducted to investigate the genetic diversity and population structure of globally diverse mini core collection of safflower (Carthamus tinctorius L.) using agro-morphological, sequence-related amplified polymorphism (SRAP) and simple-sequence repeats (SSR) molecular markers. Field experiment results showed significant differences between genotypes for agro-morphological traits such as: number of capitula per plant, number of seeds per plant, 1000-seed weight and seed yield. Ten SRAP primer combinations generated 120 polymorphic bands with an average polymorphism information content (PIC) value of 0.23. High allelic variation using 10 SSR markers observed across genotypes, with an average value of 6.8 alleles per locus and relatively high value for PIC (0.69). Pooled molecular data (SSR + SRAP) were used to identify the population structure and genetic diversity using discriminant analysis of principal components (DAPC) and STRUCTURE analysis. The results revealed four clusters within the safflower accessions, while there was no strong correlation between geographical origins and estimated genetic diversity. Our results showed high genetic diversity for both agro-morphological and molecular analysis in the examined safflower germplasm. This mini core germplasm can be used for germplasm management and future safflower breeding programs. |
| first_indexed | 2024-12-21T19:07:32Z |
| format | Article |
| id | doaj.art-af7f227fc5464dbf92db5cff1bac0d88 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-21T19:07:32Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-af7f227fc5464dbf92db5cff1bac0d882022-12-21T18:53:18ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302020-01-013411043105510.1080/13102818.2020.18186201818620Morphological description, genetic diversity and population structure of safflower (Carthamus tinctorius L.) mini core collection using SRAP and SSR markersSeyed Mohammad Reza Hassani0Reza Talebi1Sayyed Saeid Pourdad2Amir Mohammad Naji3Farzad Fayaz4Department of Agronomy and Plant Breeding, Islamic Azad University, Sanandaj BranchDepartment of Agronomy and Plant Breeding, Islamic Azad University, Sanandaj BranchDryland Agricultural Research Institute, Agricultural Research, Education and Extension Organization (AREEO)Department of Plant Breeding, College of Agriculture, Shahed UniversityDepartment of Agronomy and Plant Breeding, Islamic Azad University, Sanandaj BranchThis study was conducted to investigate the genetic diversity and population structure of globally diverse mini core collection of safflower (Carthamus tinctorius L.) using agro-morphological, sequence-related amplified polymorphism (SRAP) and simple-sequence repeats (SSR) molecular markers. Field experiment results showed significant differences between genotypes for agro-morphological traits such as: number of capitula per plant, number of seeds per plant, 1000-seed weight and seed yield. Ten SRAP primer combinations generated 120 polymorphic bands with an average polymorphism information content (PIC) value of 0.23. High allelic variation using 10 SSR markers observed across genotypes, with an average value of 6.8 alleles per locus and relatively high value for PIC (0.69). Pooled molecular data (SSR + SRAP) were used to identify the population structure and genetic diversity using discriminant analysis of principal components (DAPC) and STRUCTURE analysis. The results revealed four clusters within the safflower accessions, while there was no strong correlation between geographical origins and estimated genetic diversity. Our results showed high genetic diversity for both agro-morphological and molecular analysis in the examined safflower germplasm. This mini core germplasm can be used for germplasm management and future safflower breeding programs.http://dx.doi.org/10.1080/13102818.2020.1818620safflowergenetic diversitypopulation structuressrsrap |
| spellingShingle | Seyed Mohammad Reza Hassani Reza Talebi Sayyed Saeid Pourdad Amir Mohammad Naji Farzad Fayaz Morphological description, genetic diversity and population structure of safflower (Carthamus tinctorius L.) mini core collection using SRAP and SSR markers Biotechnology & Biotechnological Equipment safflower genetic diversity population structure ssr srap |
| title | Morphological description, genetic diversity and population structure of safflower (Carthamus tinctorius L.) mini core collection using SRAP and SSR markers |
| title_full | Morphological description, genetic diversity and population structure of safflower (Carthamus tinctorius L.) mini core collection using SRAP and SSR markers |
| title_fullStr | Morphological description, genetic diversity and population structure of safflower (Carthamus tinctorius L.) mini core collection using SRAP and SSR markers |
| title_full_unstemmed | Morphological description, genetic diversity and population structure of safflower (Carthamus tinctorius L.) mini core collection using SRAP and SSR markers |
| title_short | Morphological description, genetic diversity and population structure of safflower (Carthamus tinctorius L.) mini core collection using SRAP and SSR markers |
| title_sort | morphological description genetic diversity and population structure of safflower carthamus tinctorius l mini core collection using srap and ssr markers |
| topic | safflower genetic diversity population structure ssr srap |
| url | http://dx.doi.org/10.1080/13102818.2020.1818620 |
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