Loss-of-Function of <i>ATS1</i> Enhances Arabidopsis Salt Tolerance
Despite the importance of lipid metabolism in various biological processes, little is known about the functionality of ATS1, a plastid glycerol-3-phosphate acyltransferase catalyzing the initial step of the prokaryotic glycerolipids biosynthetic pathway, in plant response to salt stress. In this stu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/14/2646 |
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| author | Yakun Liu Guifen Wu Xingxing Ke Zhifu Zheng Yueping Zheng |
| author_facet | Yakun Liu Guifen Wu Xingxing Ke Zhifu Zheng Yueping Zheng |
| author_sort | Yakun Liu |
| collection | DOAJ |
| description | Despite the importance of lipid metabolism in various biological processes, little is known about the functionality of ATS1, a plastid glycerol-3-phosphate acyltransferase catalyzing the initial step of the prokaryotic glycerolipids biosynthetic pathway, in plant response to salt stress. In this study, both the loss-of-function mutants and the overexpression lines of <i>ATS1</i> were analyzed for salt tolerance properties. The results showed that <i>ATS1</i> overexpression lines had lower seed germination, shoot biomass, chlorophyll content, the proportion of relatively normal pod, and higher root/shoot ratio and anthocyanidin content compared with the wild type. Physiological and biochemical analysis revealed that <i>ats1</i> mutants had more unsaturated fatty acids to stabilize the plasma membrane under salt damage. Additionally, less induction of three main antioxidant enzymes activity and lower MDA content in <i>ats1</i> mutants indicated that mutation of the <i>ATS1</i> gene could reduce the damage extent. Furthermore, the <i>ats1</i> mutants maintained the K<sup>+</sup>/Na<sup>+</sup> homeostasis by upregulating <i>HAK5</i> expression to increase K<sup>+</sup> absorption and down-regulating <i>HKT1</i> expression to prevent Na<sup>+</sup> uptake. This study suggested that the <i>ATS1</i> gene negatively affects salt resistance in Arabidopsis. |
| first_indexed | 2024-03-11T00:43:15Z |
| format | Article |
| id | doaj.art-af8031a3e1354a539accb6a770b1f932 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T00:43:15Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-af8031a3e1354a539accb6a770b1f9322023-11-18T21:00:02ZengMDPI AGPlants2223-77472023-07-011214264610.3390/plants12142646Loss-of-Function of <i>ATS1</i> Enhances Arabidopsis Salt ToleranceYakun Liu0Guifen Wu1Xingxing Ke2Zhifu Zheng3Yueping Zheng4The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, ChinaCollege of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, ChinaThe Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, ChinaThe Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, ChinaThe Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, ChinaDespite the importance of lipid metabolism in various biological processes, little is known about the functionality of ATS1, a plastid glycerol-3-phosphate acyltransferase catalyzing the initial step of the prokaryotic glycerolipids biosynthetic pathway, in plant response to salt stress. In this study, both the loss-of-function mutants and the overexpression lines of <i>ATS1</i> were analyzed for salt tolerance properties. The results showed that <i>ATS1</i> overexpression lines had lower seed germination, shoot biomass, chlorophyll content, the proportion of relatively normal pod, and higher root/shoot ratio and anthocyanidin content compared with the wild type. Physiological and biochemical analysis revealed that <i>ats1</i> mutants had more unsaturated fatty acids to stabilize the plasma membrane under salt damage. Additionally, less induction of three main antioxidant enzymes activity and lower MDA content in <i>ats1</i> mutants indicated that mutation of the <i>ATS1</i> gene could reduce the damage extent. Furthermore, the <i>ats1</i> mutants maintained the K<sup>+</sup>/Na<sup>+</sup> homeostasis by upregulating <i>HAK5</i> expression to increase K<sup>+</sup> absorption and down-regulating <i>HKT1</i> expression to prevent Na<sup>+</sup> uptake. This study suggested that the <i>ATS1</i> gene negatively affects salt resistance in Arabidopsis.https://www.mdpi.com/2223-7747/12/14/2646<i>Arabidopsis thaliana</i><i>ATS1</i>salt tolerance |
| spellingShingle | Yakun Liu Guifen Wu Xingxing Ke Zhifu Zheng Yueping Zheng Loss-of-Function of <i>ATS1</i> Enhances Arabidopsis Salt Tolerance Plants <i>Arabidopsis thaliana</i> <i>ATS1</i> salt tolerance |
| title | Loss-of-Function of <i>ATS1</i> Enhances Arabidopsis Salt Tolerance |
| title_full | Loss-of-Function of <i>ATS1</i> Enhances Arabidopsis Salt Tolerance |
| title_fullStr | Loss-of-Function of <i>ATS1</i> Enhances Arabidopsis Salt Tolerance |
| title_full_unstemmed | Loss-of-Function of <i>ATS1</i> Enhances Arabidopsis Salt Tolerance |
| title_short | Loss-of-Function of <i>ATS1</i> Enhances Arabidopsis Salt Tolerance |
| title_sort | loss of function of i ats1 i enhances arabidopsis salt tolerance |
| topic | <i>Arabidopsis thaliana</i> <i>ATS1</i> salt tolerance |
| url | https://www.mdpi.com/2223-7747/12/14/2646 |
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