WAVE2 is associated with poor prognosis in pancreatic cancers and promotes cell motility and invasiveness via binding to ACTN4
Abstract WAVE2 is a member of the WASP/WAVE family of actin cytoskeletal regulatory proteins; unfortunately, little is known about its function in pancreatic cancers. In this study, we report the role of WAVE2 in the motility and invasiveness of pancreatic cancer cells. High WAVE2 expression in huma...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-11-01
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| Series: | Cancer Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/cam4.1837 |
| _version_ | 1797681467970551808 |
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| author | Keisuke Taniuchi Mutsuo Furihata Seiji Naganuma Toshiji Saibara |
| author_facet | Keisuke Taniuchi Mutsuo Furihata Seiji Naganuma Toshiji Saibara |
| author_sort | Keisuke Taniuchi |
| collection | DOAJ |
| description | Abstract WAVE2 is a member of the WASP/WAVE family of actin cytoskeletal regulatory proteins; unfortunately, little is known about its function in pancreatic cancers. In this study, we report the role of WAVE2 in the motility and invasiveness of pancreatic cancer cells. High WAVE2 expression in human pancreatic cancer tissues was correlated with overall survival. WAVE2 accumulated in the cell protrusions of pancreatic cancer cell lines. Downregulation of WAVE2 by small interfering RNA decreased the cell protrusions and inhibited the motility and invasiveness of pancreatic cancer cells. WAVE2 promoted pancreatic cancer cell motility and invasion by forming a complex with the actin cytoskeletal protein alpha‐actinin 4 (ACTN4). Downregulation of ACTN4 by small interfering RNA also inhibited the motility and invasiveness of the cells through a decrease in cell protrusions. Further investigation showed that WAVE2/ACTN4 signaling selectively stimulated p27 phosphorylation and thereby increased the motility and invasiveness of the cells. These results suggest that WAVE2 and ACTN4 stimulate p27 phosphorylation and provide evidence that WAVE2 promotes the motility and invasiveness of pancreatic cancer cells. |
| first_indexed | 2024-03-11T23:45:16Z |
| format | Article |
| id | doaj.art-af80c4bba28345dfbbd3e58da09a1c73 |
| institution | Directory Open Access Journal |
| issn | 2045-7634 |
| language | English |
| last_indexed | 2024-03-11T23:45:16Z |
| publishDate | 2018-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj.art-af80c4bba28345dfbbd3e58da09a1c732023-09-19T11:30:57ZengWileyCancer Medicine2045-76342018-11-017115733575110.1002/cam4.1837WAVE2 is associated with poor prognosis in pancreatic cancers and promotes cell motility and invasiveness via binding to ACTN4Keisuke Taniuchi0Mutsuo Furihata1Seiji Naganuma2Toshiji Saibara3Department of Gastroenterology and Hepatology, Kochi Medical School Kochi University Kochi JapanDepartment of Pathology, Kochi Medical School Kochi University Kochi JapanDepartment of Pathology, Kochi Medical School Kochi University Kochi JapanDepartment of Gastroenterology and Hepatology, Kochi Medical School Kochi University Kochi JapanAbstract WAVE2 is a member of the WASP/WAVE family of actin cytoskeletal regulatory proteins; unfortunately, little is known about its function in pancreatic cancers. In this study, we report the role of WAVE2 in the motility and invasiveness of pancreatic cancer cells. High WAVE2 expression in human pancreatic cancer tissues was correlated with overall survival. WAVE2 accumulated in the cell protrusions of pancreatic cancer cell lines. Downregulation of WAVE2 by small interfering RNA decreased the cell protrusions and inhibited the motility and invasiveness of pancreatic cancer cells. WAVE2 promoted pancreatic cancer cell motility and invasion by forming a complex with the actin cytoskeletal protein alpha‐actinin 4 (ACTN4). Downregulation of ACTN4 by small interfering RNA also inhibited the motility and invasiveness of the cells through a decrease in cell protrusions. Further investigation showed that WAVE2/ACTN4 signaling selectively stimulated p27 phosphorylation and thereby increased the motility and invasiveness of the cells. These results suggest that WAVE2 and ACTN4 stimulate p27 phosphorylation and provide evidence that WAVE2 promotes the motility and invasiveness of pancreatic cancer cells.https://doi.org/10.1002/cam4.1837actin cytoskeletonACTN4cell invasionp27pancreatic cancerWAVE2 |
| spellingShingle | Keisuke Taniuchi Mutsuo Furihata Seiji Naganuma Toshiji Saibara WAVE2 is associated with poor prognosis in pancreatic cancers and promotes cell motility and invasiveness via binding to ACTN4 Cancer Medicine actin cytoskeleton ACTN4 cell invasion p27 pancreatic cancer WAVE2 |
| title | WAVE2 is associated with poor prognosis in pancreatic cancers and promotes cell motility and invasiveness via binding to ACTN4 |
| title_full | WAVE2 is associated with poor prognosis in pancreatic cancers and promotes cell motility and invasiveness via binding to ACTN4 |
| title_fullStr | WAVE2 is associated with poor prognosis in pancreatic cancers and promotes cell motility and invasiveness via binding to ACTN4 |
| title_full_unstemmed | WAVE2 is associated with poor prognosis in pancreatic cancers and promotes cell motility and invasiveness via binding to ACTN4 |
| title_short | WAVE2 is associated with poor prognosis in pancreatic cancers and promotes cell motility and invasiveness via binding to ACTN4 |
| title_sort | wave2 is associated with poor prognosis in pancreatic cancers and promotes cell motility and invasiveness via binding to actn4 |
| topic | actin cytoskeleton ACTN4 cell invasion p27 pancreatic cancer WAVE2 |
| url | https://doi.org/10.1002/cam4.1837 |
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