Detection of polymorphism within leptin gene in Egyptian river buffalo and predict its effects on different molecular levels
Abstract Background Leptin (LEP) regulates the glucose homeostasis directly and centrally by the regulation of the insulin levels or indirectly by alternation of the levels of the other glucose metabolism regulator hormones. The present investigation studied the polymorphism in LEP gene which is rel...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-02-01
|
| Series: | Journal of Genetic Engineering and Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43141-020-0020-5 |
| _version_ | 1797203589660147712 |
|---|---|
| author | Karima F. Mahrous Mohamad M. Aboelenin Mohamed A. Rashed Mahmoud A. Sallam Hossam E. Rushdi |
| author_facet | Karima F. Mahrous Mohamad M. Aboelenin Mohamed A. Rashed Mahmoud A. Sallam Hossam E. Rushdi |
| author_sort | Karima F. Mahrous |
| collection | DOAJ |
| description | Abstract Background Leptin (LEP) regulates the glucose homeostasis directly and centrally by the regulation of the insulin levels or indirectly by alternation of the levels of the other glucose metabolism regulator hormones. The present investigation studied the polymorphism in LEP gene which is related to fertility in 81 female Egyptian river buffalo. Results The PCR-RFLP pattern of the gene using the restriction enzyme Eco91I showed that all the animals had monomorphic pattern in the studied gene which consists of CC. A 511-bp fragment from LEP gene was amplified and sequenced. The homology between the amplified LEP gene fragment in buffalo and cattle, sheep, goat, human, and mouse on the nucleotides sequence level was 99, 97, 97, 87, and 79%, respectively, and on the translated amino acids sequence level was 100, 98, 98, 85, and 82%, respectively. Several SNPs were detected; among them, the T27C SNP disrupted an intronic splicing silencer. The A114G, A310G, G263A, and G379A SNPs disrupt exonic splicing enhancers, and the last two SNPs create new exonic splicing enhancers. The A114G, C163A, A211G, G288A, A310G, A322G, G330C, C348T, T360C, and G379A SNPs cause S71G, T87 N, N103S, E129K, E136G, Y140C, E143Q, R149W, S153P, and R159Q amino acids mutations. N103S, E129K, E136G, Y140C, E143Q, and S153P were classified as deleterious mutations. Y140, E143, N103, and R149 were the most conserved among the mutated amino acids. S71G only increased the stability of the leptin protein while the remaining mutations decreased it. Conclusion Four SNPs were revealed among the tested animals. Twenty-one SNPs were found between the sequenced amplicon and the buffalo records in the Genbank. Some SNPs were predicted to have several effects on different biological processes like mRNA splicing, protein stability, and the gene functions. |
| first_indexed | 2024-04-24T08:21:44Z |
| format | Article |
| id | doaj.art-af8711b393134cedbe29e38b5ca38857 |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-04-24T08:21:44Z |
| publishDate | 2020-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-af8711b393134cedbe29e38b5ca388572024-04-17T00:54:54ZengElsevierJournal of Genetic Engineering and Biotechnology2090-59202020-02-0118111110.1186/s43141-020-0020-5Detection of polymorphism within leptin gene in Egyptian river buffalo and predict its effects on different molecular levelsKarima F. Mahrous0Mohamad M. Aboelenin1Mohamed A. Rashed2Mahmoud A. Sallam3Hossam E. Rushdi4Cell Biology Department, National Research CentreCell Biology Department, National Research CentreDepartment of Genetics, Faculty of Agriculture, Ain Shams UniversityDepartment of Genetics, Faculty of Agriculture, Ain Shams UniversityDepartment of Animal Production, Faculty of Agriculture, Cairo UniversityAbstract Background Leptin (LEP) regulates the glucose homeostasis directly and centrally by the regulation of the insulin levels or indirectly by alternation of the levels of the other glucose metabolism regulator hormones. The present investigation studied the polymorphism in LEP gene which is related to fertility in 81 female Egyptian river buffalo. Results The PCR-RFLP pattern of the gene using the restriction enzyme Eco91I showed that all the animals had monomorphic pattern in the studied gene which consists of CC. A 511-bp fragment from LEP gene was amplified and sequenced. The homology between the amplified LEP gene fragment in buffalo and cattle, sheep, goat, human, and mouse on the nucleotides sequence level was 99, 97, 97, 87, and 79%, respectively, and on the translated amino acids sequence level was 100, 98, 98, 85, and 82%, respectively. Several SNPs were detected; among them, the T27C SNP disrupted an intronic splicing silencer. The A114G, A310G, G263A, and G379A SNPs disrupt exonic splicing enhancers, and the last two SNPs create new exonic splicing enhancers. The A114G, C163A, A211G, G288A, A310G, A322G, G330C, C348T, T360C, and G379A SNPs cause S71G, T87 N, N103S, E129K, E136G, Y140C, E143Q, R149W, S153P, and R159Q amino acids mutations. N103S, E129K, E136G, Y140C, E143Q, and S153P were classified as deleterious mutations. Y140, E143, N103, and R149 were the most conserved among the mutated amino acids. S71G only increased the stability of the leptin protein while the remaining mutations decreased it. Conclusion Four SNPs were revealed among the tested animals. Twenty-one SNPs were found between the sequenced amplicon and the buffalo records in the Genbank. Some SNPs were predicted to have several effects on different biological processes like mRNA splicing, protein stability, and the gene functions.https://doi.org/10.1186/s43141-020-0020-5Egyptian river buffaloesLeptinPCR-RFLPSNP |
| spellingShingle | Karima F. Mahrous Mohamad M. Aboelenin Mohamed A. Rashed Mahmoud A. Sallam Hossam E. Rushdi Detection of polymorphism within leptin gene in Egyptian river buffalo and predict its effects on different molecular levels Journal of Genetic Engineering and Biotechnology Egyptian river buffaloes Leptin PCR-RFLP SNP |
| title | Detection of polymorphism within leptin gene in Egyptian river buffalo and predict its effects on different molecular levels |
| title_full | Detection of polymorphism within leptin gene in Egyptian river buffalo and predict its effects on different molecular levels |
| title_fullStr | Detection of polymorphism within leptin gene in Egyptian river buffalo and predict its effects on different molecular levels |
| title_full_unstemmed | Detection of polymorphism within leptin gene in Egyptian river buffalo and predict its effects on different molecular levels |
| title_short | Detection of polymorphism within leptin gene in Egyptian river buffalo and predict its effects on different molecular levels |
| title_sort | detection of polymorphism within leptin gene in egyptian river buffalo and predict its effects on different molecular levels |
| topic | Egyptian river buffaloes Leptin PCR-RFLP SNP |
| url | https://doi.org/10.1186/s43141-020-0020-5 |
| work_keys_str_mv | AT karimafmahrous detectionofpolymorphismwithinleptingeneinegyptianriverbuffaloandpredictitseffectsondifferentmolecularlevels AT mohamadmaboelenin detectionofpolymorphismwithinleptingeneinegyptianriverbuffaloandpredictitseffectsondifferentmolecularlevels AT mohamedarashed detectionofpolymorphismwithinleptingeneinegyptianriverbuffaloandpredictitseffectsondifferentmolecularlevels AT mahmoudasallam detectionofpolymorphismwithinleptingeneinegyptianriverbuffaloandpredictitseffectsondifferentmolecularlevels AT hossamerushdi detectionofpolymorphismwithinleptingeneinegyptianriverbuffaloandpredictitseffectsondifferentmolecularlevels |