Evaluasi Keragaman Genetik Gen Hormon Pertumbuhan (GH) pada Sapi Pesisir Sumatera Barat Menggunakan Penciri PCR-RFLP
A total of 134 Pesisir cattle were genotyped for growth hormone (GH) gene by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The genotype and allele frequencies of the GH MspI and GH AluI Pesisir cattle were determined. The GH MspI gene frequencies for the C and T alle...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2007-04-01
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| Series: | Media Peternakan |
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| Online Access: | http://medpet.journal.ipb.ac.id/index.php/mediapeternakan/article/view/1014 |
| _version_ | 1818051350324314112 |
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| author | Jakaria D. Duryadib R.R. Noor B. Tappa H. Martojo |
| author_facet | Jakaria D. Duryadib R.R. Noor B. Tappa H. Martojo |
| author_sort | Jakaria |
| collection | DOAJ |
| description | A total of 134 Pesisir cattle were genotyped for growth hormone (GH) gene by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The genotype and allele frequencies of the GH MspI and GH AluI Pesisir cattle were determined. The GH MspI gene frequencies for the C and T allele were 0.209 and 0.791 respectively, while GH AluI gene frequencies for the L and V allele were 0.992 and 0.008 respectively. The chi-square analysis indicated that this population is not in Hardy-Weinberg Equilibrium status. Expected heterozygosis value (He) for GH MspI and GH AluI were 0.3306±0.0266 and 0.0149±0.0073 respectively. The PCR-RFLP GH MspI marker has higher genetic variability compare to PCR-RFLP AluI marker. This finding showed that GH MspI T allele was favorable as a GH marker for Bos indicus breeds. |
| first_indexed | 2024-12-10T11:07:58Z |
| format | Article |
| id | doaj.art-af8b3088a0454d6cbc042a155a73fd8e |
| institution | Directory Open Access Journal |
| issn | 0126-0472 2087-4634 |
| language | English |
| last_indexed | 2024-12-10T11:07:58Z |
| publishDate | 2007-04-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Media Peternakan |
| spelling | doaj.art-af8b3088a0454d6cbc042a155a73fd8e2022-12-22T01:51:31ZengBogor Agricultural UniversityMedia Peternakan0126-04722087-46342007-04-01301110Evaluasi Keragaman Genetik Gen Hormon Pertumbuhan (GH) pada Sapi Pesisir Sumatera Barat Menggunakan Penciri PCR-RFLPJakariaD. DuryadibR.R. NoorB. TappaH. MartojoA total of 134 Pesisir cattle were genotyped for growth hormone (GH) gene by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The genotype and allele frequencies of the GH MspI and GH AluI Pesisir cattle were determined. The GH MspI gene frequencies for the C and T allele were 0.209 and 0.791 respectively, while GH AluI gene frequencies for the L and V allele were 0.992 and 0.008 respectively. The chi-square analysis indicated that this population is not in Hardy-Weinberg Equilibrium status. Expected heterozygosis value (He) for GH MspI and GH AluI were 0.3306±0.0266 and 0.0149±0.0073 respectively. The PCR-RFLP GH MspI marker has higher genetic variability compare to PCR-RFLP AluI marker. This finding showed that GH MspI T allele was favorable as a GH marker for Bos indicus breeds.http://medpet.journal.ipb.ac.id/index.php/mediapeternakan/article/view/1014Pesisir cattlegrowth hormone genePCR-RFLPpolymorphism |
| spellingShingle | Jakaria D. Duryadib R.R. Noor B. Tappa H. Martojo Evaluasi Keragaman Genetik Gen Hormon Pertumbuhan (GH) pada Sapi Pesisir Sumatera Barat Menggunakan Penciri PCR-RFLP Media Peternakan Pesisir cattle growth hormone gene PCR-RFLP polymorphism |
| title | Evaluasi Keragaman Genetik Gen Hormon Pertumbuhan (GH) pada Sapi Pesisir Sumatera Barat Menggunakan Penciri PCR-RFLP |
| title_full | Evaluasi Keragaman Genetik Gen Hormon Pertumbuhan (GH) pada Sapi Pesisir Sumatera Barat Menggunakan Penciri PCR-RFLP |
| title_fullStr | Evaluasi Keragaman Genetik Gen Hormon Pertumbuhan (GH) pada Sapi Pesisir Sumatera Barat Menggunakan Penciri PCR-RFLP |
| title_full_unstemmed | Evaluasi Keragaman Genetik Gen Hormon Pertumbuhan (GH) pada Sapi Pesisir Sumatera Barat Menggunakan Penciri PCR-RFLP |
| title_short | Evaluasi Keragaman Genetik Gen Hormon Pertumbuhan (GH) pada Sapi Pesisir Sumatera Barat Menggunakan Penciri PCR-RFLP |
| title_sort | evaluasi keragaman genetik gen hormon pertumbuhan gh pada sapi pesisir sumatera barat menggunakan penciri pcr rflp |
| topic | Pesisir cattle growth hormone gene PCR-RFLP polymorphism |
| url | http://medpet.journal.ipb.ac.id/index.php/mediapeternakan/article/view/1014 |
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