Reagent Effects on the Activated Partial Thromboplastin Time Clot Waveform Analysis: A Multi-Centre Study
(1) Background: The activated partial thromboplastin time (APTT)- based clot waveform analysis (CWA) quantitatively extends information obtained from the APTT waveform through its derivatives. However, pre-analytical variables including reagent effects on the CWA parameters are poorly understood and...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | Diagnostics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4418/13/14/2447 |
| _version_ | 1797589679255584768 |
|---|---|
| author | Wan Hui Wong Chuen Wen Tan Nabeelah Binti Abdul Khalid Nadjwa Zamalek Dalimoenthe Christina Yip Chaicharoen Tantanate Rodelio D. Lim Ji Hyun Kim Heng Joo Ng |
| author_facet | Wan Hui Wong Chuen Wen Tan Nabeelah Binti Abdul Khalid Nadjwa Zamalek Dalimoenthe Christina Yip Chaicharoen Tantanate Rodelio D. Lim Ji Hyun Kim Heng Joo Ng |
| author_sort | Wan Hui Wong |
| collection | DOAJ |
| description | (1) Background: The activated partial thromboplastin time (APTT)- based clot waveform analysis (CWA) quantitatively extends information obtained from the APTT waveform through its derivatives. However, pre-analytical variables including reagent effects on the CWA parameters are poorly understood and must be standardized as a potential diagnostic assay. (2) Methods: CWA was first analysed with patient samples to understand reagent lot variation in three common APTT reagents: Pathromtin SL, Actin FS, and Actin FSL. A total of 1055 healthy volunteers were also recruited from seven institutions across the Asia-Pacific region and CWA data were collected with the Sysmex CS analysers. (3) Results: CWA parameters varied less than 10% between lots and the linear mixed model analysis showed few site-specific effects within the same reagent group. However, the CWA parameters were significantly different amongst all reagent groups and thus reagent-specific 95% reference intervals could be calculated using the nonparametric method. Post-hoc analysis showed some degree of influence by age and gender with weak correlation to the CWA (r < 0.3). (4) Conclusions: Reagent type significantly affects APTT-based CWA with minimal inter-laboratory variations with the same coagulometer series that allow for data pooling across laboratories with more evidence required for age- and gender-partitioning. |
| first_indexed | 2024-03-11T01:09:58Z |
| format | Article |
| id | doaj.art-af8be2d7ddc74895995c6ada99a4187e |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-11T01:09:58Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-af8be2d7ddc74895995c6ada99a4187e2023-11-18T18:58:59ZengMDPI AGDiagnostics2075-44182023-07-011314244710.3390/diagnostics13142447Reagent Effects on the Activated Partial Thromboplastin Time Clot Waveform Analysis: A Multi-Centre StudyWan Hui Wong0Chuen Wen Tan1Nabeelah Binti Abdul Khalid2Nadjwa Zamalek Dalimoenthe3Christina Yip4Chaicharoen Tantanate5Rodelio D. Lim6Ji Hyun Kim7Heng Joo Ng8Department of Haematology, Singapore General Hospital, Singapore 169608, SingaporeDepartment of Haematology, Singapore General Hospital, Singapore 169608, SingaporeDepartment of Pathology, Hospital Pulau Pinang, Pulau Pinang 10990, MalaysiaDepartment of Haematology, Dr Hasan Sadikin Hospital, Bandung 40161, IndonesiaDepartment of Laboratory Medicine, National University Hospital, Singapore 119074, SingaporeDepartment of Clinical Pathology, Siriraj Hospital, Bangkok 10700, ThailandInstitute of Pathology, St Luke’s Medical Centre, Global City 1634, PhilippinesDepartment of Pathology, Sengkang General Hospital, Singapore 544886, SingaporeDepartment of Haematology, Singapore General Hospital, Singapore 169608, Singapore(1) Background: The activated partial thromboplastin time (APTT)- based clot waveform analysis (CWA) quantitatively extends information obtained from the APTT waveform through its derivatives. However, pre-analytical variables including reagent effects on the CWA parameters are poorly understood and must be standardized as a potential diagnostic assay. (2) Methods: CWA was first analysed with patient samples to understand reagent lot variation in three common APTT reagents: Pathromtin SL, Actin FS, and Actin FSL. A total of 1055 healthy volunteers were also recruited from seven institutions across the Asia-Pacific region and CWA data were collected with the Sysmex CS analysers. (3) Results: CWA parameters varied less than 10% between lots and the linear mixed model analysis showed few site-specific effects within the same reagent group. However, the CWA parameters were significantly different amongst all reagent groups and thus reagent-specific 95% reference intervals could be calculated using the nonparametric method. Post-hoc analysis showed some degree of influence by age and gender with weak correlation to the CWA (r < 0.3). (4) Conclusions: Reagent type significantly affects APTT-based CWA with minimal inter-laboratory variations with the same coagulometer series that allow for data pooling across laboratories with more evidence required for age- and gender-partitioning.https://www.mdpi.com/2075-4418/13/14/2447clotwaveform analysiscoagulationactivated partial thromboplastin timereagentreference interval |
| spellingShingle | Wan Hui Wong Chuen Wen Tan Nabeelah Binti Abdul Khalid Nadjwa Zamalek Dalimoenthe Christina Yip Chaicharoen Tantanate Rodelio D. Lim Ji Hyun Kim Heng Joo Ng Reagent Effects on the Activated Partial Thromboplastin Time Clot Waveform Analysis: A Multi-Centre Study Diagnostics clot waveform analysis coagulation activated partial thromboplastin time reagent reference interval |
| title | Reagent Effects on the Activated Partial Thromboplastin Time Clot Waveform Analysis: A Multi-Centre Study |
| title_full | Reagent Effects on the Activated Partial Thromboplastin Time Clot Waveform Analysis: A Multi-Centre Study |
| title_fullStr | Reagent Effects on the Activated Partial Thromboplastin Time Clot Waveform Analysis: A Multi-Centre Study |
| title_full_unstemmed | Reagent Effects on the Activated Partial Thromboplastin Time Clot Waveform Analysis: A Multi-Centre Study |
| title_short | Reagent Effects on the Activated Partial Thromboplastin Time Clot Waveform Analysis: A Multi-Centre Study |
| title_sort | reagent effects on the activated partial thromboplastin time clot waveform analysis a multi centre study |
| topic | clot waveform analysis coagulation activated partial thromboplastin time reagent reference interval |
| url | https://www.mdpi.com/2075-4418/13/14/2447 |
| work_keys_str_mv | AT wanhuiwong reagenteffectsontheactivatedpartialthromboplastintimeclotwaveformanalysisamulticentrestudy AT chuenwentan reagenteffectsontheactivatedpartialthromboplastintimeclotwaveformanalysisamulticentrestudy AT nabeelahbintiabdulkhalid reagenteffectsontheactivatedpartialthromboplastintimeclotwaveformanalysisamulticentrestudy AT nadjwazamalekdalimoenthe reagenteffectsontheactivatedpartialthromboplastintimeclotwaveformanalysisamulticentrestudy AT christinayip reagenteffectsontheactivatedpartialthromboplastintimeclotwaveformanalysisamulticentrestudy AT chaicharoentantanate reagenteffectsontheactivatedpartialthromboplastintimeclotwaveformanalysisamulticentrestudy AT rodeliodlim reagenteffectsontheactivatedpartialthromboplastintimeclotwaveformanalysisamulticentrestudy AT jihyunkim reagenteffectsontheactivatedpartialthromboplastintimeclotwaveformanalysisamulticentrestudy AT hengjoong reagenteffectsontheactivatedpartialthromboplastintimeclotwaveformanalysisamulticentrestudy |