Arginine Alters miRNA Expression Involved in Development and Proliferation of Rat Mammary Tissue
This study was designed to determine the effects of dietary arginine on development and proliferation in rat mammary tissue through changes in miRNA profiles. Twelve pregnant Wistar rats were allocated randomly to two groups. A basal diet containing arginine or the control diet containing glutamate...
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MDPI AG
2021-02-01
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| Online Access: | https://www.mdpi.com/2076-2615/11/2/535 |
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| author | Gang Zhou Qiaoyun Xu Feifan Wu Mengzhi Wang Lianmin Chen Liangyu Hu Jingwen Zhao Juan J. Loor Jun Zhang |
| author_facet | Gang Zhou Qiaoyun Xu Feifan Wu Mengzhi Wang Lianmin Chen Liangyu Hu Jingwen Zhao Juan J. Loor Jun Zhang |
| author_sort | Gang Zhou |
| collection | DOAJ |
| description | This study was designed to determine the effects of dietary arginine on development and proliferation in rat mammary tissue through changes in miRNA profiles. Twelve pregnant Wistar rats were allocated randomly to two groups. A basal diet containing arginine or the control diet containing glutamate on an equal nitrogen basis as the arginine supplemented diet were used. The experiment included a pre-experimental period of four days before parturition and an experimental period of 17 days after parturition. Mammary tissue was collected for histology, RNA extraction and high-throughput sequencing analysis. The greater mammary acinar area indicated that arginine supplementation enhanced mammary tissue development (<i>p</i> < 0.01). MicroRNA profiling indicated that seven miRNA (miR-206-3p, miR-133a-5p, miR-133b-3p, miR-1-3p, miR-133a-3p, miR-1b and miR-486) were differentially expressed in response to Arginine when compared with the glutamate-based control group. In silico gene ontology enrichment and KEGG pathway analysis revealed between 240 and 535 putative target genes among the miRNA. Further verification by qPCR revealed concordance with the differential expression from the sequencing results: 17 of 28 target genes were differentially expressed (15 were highly expressed in arginine and 2 in control) and 11 target genes did not have significant difference in expression. In conclusion, our study suggests that arginine may potentially regulate the development of rat mammary glands through regulating miRNAs. |
| first_indexed | 2024-03-09T00:45:56Z |
| format | Article |
| id | doaj.art-af8c5ad7fb4142f4bdec1d9c8189cf0c |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T00:45:56Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-af8c5ad7fb4142f4bdec1d9c8189cf0c2023-12-11T17:33:11ZengMDPI AGAnimals2076-26152021-02-0111253510.3390/ani11020535Arginine Alters miRNA Expression Involved in Development and Proliferation of Rat Mammary TissueGang Zhou0Qiaoyun Xu1Feifan Wu2Mengzhi Wang3Lianmin Chen4Liangyu Hu5Jingwen Zhao6Juan J. Loor7Jun Zhang8Huaiyin Institute of Agricultural Sciences in Xuhuai Regio, Huaian 223000, ChinaCollege of Animal Science and Technology, Yangzhou University, 88 South University Ave., Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, 88 South University Ave., Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, 88 South University Ave., Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, 88 South University Ave., Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, 88 South University Ave., Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, 88 South University Ave., Yangzhou 225009, ChinaDepartment of Animal Sciences and Division of Nutritional Sciences, University of Illinois, 1207 W Gregory Drive, Urbana, IL 61801, USACollege of Animal Science and Technology, Yangzhou University, 88 South University Ave., Yangzhou 225009, ChinaThis study was designed to determine the effects of dietary arginine on development and proliferation in rat mammary tissue through changes in miRNA profiles. Twelve pregnant Wistar rats were allocated randomly to two groups. A basal diet containing arginine or the control diet containing glutamate on an equal nitrogen basis as the arginine supplemented diet were used. The experiment included a pre-experimental period of four days before parturition and an experimental period of 17 days after parturition. Mammary tissue was collected for histology, RNA extraction and high-throughput sequencing analysis. The greater mammary acinar area indicated that arginine supplementation enhanced mammary tissue development (<i>p</i> < 0.01). MicroRNA profiling indicated that seven miRNA (miR-206-3p, miR-133a-5p, miR-133b-3p, miR-1-3p, miR-133a-3p, miR-1b and miR-486) were differentially expressed in response to Arginine when compared with the glutamate-based control group. In silico gene ontology enrichment and KEGG pathway analysis revealed between 240 and 535 putative target genes among the miRNA. Further verification by qPCR revealed concordance with the differential expression from the sequencing results: 17 of 28 target genes were differentially expressed (15 were highly expressed in arginine and 2 in control) and 11 target genes did not have significant difference in expression. In conclusion, our study suggests that arginine may potentially regulate the development of rat mammary glands through regulating miRNAs.https://www.mdpi.com/2076-2615/11/2/535arginineratmammarymiRNAs |
| spellingShingle | Gang Zhou Qiaoyun Xu Feifan Wu Mengzhi Wang Lianmin Chen Liangyu Hu Jingwen Zhao Juan J. Loor Jun Zhang Arginine Alters miRNA Expression Involved in Development and Proliferation of Rat Mammary Tissue Animals arginine rat mammary miRNAs |
| title | Arginine Alters miRNA Expression Involved in Development and Proliferation of Rat Mammary Tissue |
| title_full | Arginine Alters miRNA Expression Involved in Development and Proliferation of Rat Mammary Tissue |
| title_fullStr | Arginine Alters miRNA Expression Involved in Development and Proliferation of Rat Mammary Tissue |
| title_full_unstemmed | Arginine Alters miRNA Expression Involved in Development and Proliferation of Rat Mammary Tissue |
| title_short | Arginine Alters miRNA Expression Involved in Development and Proliferation of Rat Mammary Tissue |
| title_sort | arginine alters mirna expression involved in development and proliferation of rat mammary tissue |
| topic | arginine rat mammary miRNAs |
| url | https://www.mdpi.com/2076-2615/11/2/535 |
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