Jatropha curcas leaves exert anti-arthritic activity on adjuvant-induced arthritis in rats
BACKGROUND Jatropha curcas leaves have been proven to be anti-inflammatory and antioxidant. In this study we examined the antiarthritic effects of ethanolic extract of J. curcas leaves using adjuvant induced arthritis (AIA) in rats. METHODS Male Wistar rats were divided into 6 groups (n=8), co...
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| Format: | Article |
| Language: | English |
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Faculty of Medicine Trisakti University
2014-04-01
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| Series: | Universa Medicina |
| Subjects: | |
| Online Access: | http://www.univmed.org/wp-content/uploads/2014/05/Hanif1.pdf |
| _version_ | 1818210694460342272 |
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| author | Hanif Nasiatul Baroroh Iskandar Sobri Eka Prasasti Nur Rachmani Triana Hertiani Zullies Ikawati |
| author_facet | Hanif Nasiatul Baroroh Iskandar Sobri Eka Prasasti Nur Rachmani Triana Hertiani Zullies Ikawati |
| author_sort | Hanif Nasiatul Baroroh |
| collection | DOAJ |
| description | BACKGROUND
Jatropha curcas leaves have been proven to be anti-inflammatory and antioxidant. In this study we examined the antiarthritic effects of ethanolic extract of J. curcas leaves using adjuvant induced arthritis (AIA) in rats.
METHODS
Male Wistar rats were divided into 6 groups (n=8), consisting of normal group (0.9% NaCl), control group (complete Freund’s adjuvant/CFA 1 mg/ml), sodium diclofenac group at a dose of 6.75 mg/kg (p.o), ethanolic extract of J.curcas groups at doses of 150 mg/kg (p.o), 300 mg/kg (p.o) and 600 mg/kg (p.o). Each group was induced by 0.2 ml CFA on day 1 and a booster injection on day 5. Extracts of J. curcas were administered on days 14-28. Arthritic scores were determined, then analyzed using Kruskal Wallis followed by Mann Whitney tests. Mobility scores were analyzed using one way analysis of variance, followed by least significant difference multiple comparison test. Arthritic joint histopathology was observed on day 29.
RESULTS
The results showed that the ethanolic extract of J. curcas leaves at doses of 150 mg/kg, 300 mg/kg and 600 mg/kg significantly reduced arthritis scores (p<0.05) compared to control group (CFA). The J. curcas leaf extract at doses of 150 and 300 mg/kg BW decreased mobility scores. Histopathology studies showed that the J. curcas extract reduced edema and cartilage destruction in arthritic joints.
CONCLUSIONS
The J. curcas leaf extract had anti-arthritic effects by reducing arthritis scores and mobility scores. The extract should be further examined as a potential candidate for anti-arthritic therapies. |
| first_indexed | 2024-12-12T05:20:41Z |
| format | Article |
| id | doaj.art-af8e61e5f3b54da882aeed63fc61f192 |
| institution | Directory Open Access Journal |
| issn | 1907-3062 |
| language | English |
| last_indexed | 2024-12-12T05:20:41Z |
| publishDate | 2014-04-01 |
| publisher | Faculty of Medicine Trisakti University |
| record_format | Article |
| series | Universa Medicina |
| spelling | doaj.art-af8e61e5f3b54da882aeed63fc61f1922022-12-22T00:36:37ZengFaculty of Medicine Trisakti UniversityUniversa Medicina1907-30622014-04-01331310Jatropha curcas leaves exert anti-arthritic activity on adjuvant-induced arthritis in ratsHanif Nasiatul Baroroh0Iskandar Sobri1Eka Prasasti Nur Rachmani2Triana Hertiani3Zullies Ikawati4Department of Pharmacy, Faculty of Medicine and Health Science,s Jenderal Soedirman University PurwokertoDepartment of Pharmacy, Faculty of Medicine and Health Science,s Jenderal Soedirman University PurwokertoDepartment of Pharmacy, Faculty of Medicine and Health Science,s Jenderal Soedirman University PurwokertoFaculty of Pharmacy, Gadjah Mada University YogyakartaFaculty of Pharmacy, Gadjah Mada University YogyakartaBACKGROUND Jatropha curcas leaves have been proven to be anti-inflammatory and antioxidant. In this study we examined the antiarthritic effects of ethanolic extract of J. curcas leaves using adjuvant induced arthritis (AIA) in rats. METHODS Male Wistar rats were divided into 6 groups (n=8), consisting of normal group (0.9% NaCl), control group (complete Freund’s adjuvant/CFA 1 mg/ml), sodium diclofenac group at a dose of 6.75 mg/kg (p.o), ethanolic extract of J.curcas groups at doses of 150 mg/kg (p.o), 300 mg/kg (p.o) and 600 mg/kg (p.o). Each group was induced by 0.2 ml CFA on day 1 and a booster injection on day 5. Extracts of J. curcas were administered on days 14-28. Arthritic scores were determined, then analyzed using Kruskal Wallis followed by Mann Whitney tests. Mobility scores were analyzed using one way analysis of variance, followed by least significant difference multiple comparison test. Arthritic joint histopathology was observed on day 29. RESULTS The results showed that the ethanolic extract of J. curcas leaves at doses of 150 mg/kg, 300 mg/kg and 600 mg/kg significantly reduced arthritis scores (p<0.05) compared to control group (CFA). The J. curcas leaf extract at doses of 150 and 300 mg/kg BW decreased mobility scores. Histopathology studies showed that the J. curcas extract reduced edema and cartilage destruction in arthritic joints. CONCLUSIONS The J. curcas leaf extract had anti-arthritic effects by reducing arthritis scores and mobility scores. The extract should be further examined as a potential candidate for anti-arthritic therapies.http://www.univmed.org/wp-content/uploads/2014/05/Hanif1.pdfAntiarthriticJatropha curcasadjuvant induced arthritisrats |
| spellingShingle | Hanif Nasiatul Baroroh Iskandar Sobri Eka Prasasti Nur Rachmani Triana Hertiani Zullies Ikawati Jatropha curcas leaves exert anti-arthritic activity on adjuvant-induced arthritis in rats Universa Medicina Antiarthritic Jatropha curcas adjuvant induced arthritis rats |
| title | Jatropha curcas leaves exert anti-arthritic activity on adjuvant-induced arthritis in rats |
| title_full | Jatropha curcas leaves exert anti-arthritic activity on adjuvant-induced arthritis in rats |
| title_fullStr | Jatropha curcas leaves exert anti-arthritic activity on adjuvant-induced arthritis in rats |
| title_full_unstemmed | Jatropha curcas leaves exert anti-arthritic activity on adjuvant-induced arthritis in rats |
| title_short | Jatropha curcas leaves exert anti-arthritic activity on adjuvant-induced arthritis in rats |
| title_sort | jatropha curcas leaves exert anti arthritic activity on adjuvant induced arthritis in rats |
| topic | Antiarthritic Jatropha curcas adjuvant induced arthritis rats |
| url | http://www.univmed.org/wp-content/uploads/2014/05/Hanif1.pdf |
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