Isolation and characterization of small extracellular vesicles from murine primary mammary tumor
Summary: Tumor-derived small extracellular vesicles (TEVs) play a pivotal role in cancer progression by transferring functional biomolecules between the parental and recipient cells. Here, we present a protocol to isolate TEVs directly from murine primary mammary tumor using differential centrifugat...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723007505 |
| _version_ | 1797388696288231424 |
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| author | Sheng Hu Xiaohui Zhang Wei Yan |
| author_facet | Sheng Hu Xiaohui Zhang Wei Yan |
| author_sort | Sheng Hu |
| collection | DOAJ |
| description | Summary: Tumor-derived small extracellular vesicles (TEVs) play a pivotal role in cancer progression by transferring functional biomolecules between the parental and recipient cells. Here, we present a protocol to isolate TEVs directly from murine primary mammary tumor using differential centrifugation. We describe steps for tissue dissociation, enzymatic digestion, and centrifugation. We then detail procedures for characterization of TEVs through transmission electron microscopy, immunoblotting, and nano-flow cytometry. This protocol can be used to extract EVs from other solid tumor types.For complete details on the use and execution of this protocol, please refer to Li, Mei-Xin et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-08T22:44:33Z |
| format | Article |
| id | doaj.art-af96d0be368b460c8d58f7ec0c9f4d5f |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-08T22:44:33Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-af96d0be368b460c8d58f7ec0c9f4d5f2023-12-17T06:41:48ZengElsevierSTAR Protocols2666-16672024-03-0151102783Isolation and characterization of small extracellular vesicles from murine primary mammary tumorSheng Hu0Xiaohui Zhang1Wei Yan2Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, TaiKang Center for Life and Medical Sciences, Wuhan University, Wuhan 430072, ChinaHubei Key Laboratory of Cell Homeostasis, College of Life Sciences, TaiKang Center for Life and Medical Sciences, Wuhan University, Wuhan 430072, ChinaHubei Key Laboratory of Cell Homeostasis, College of Life Sciences, TaiKang Center for Life and Medical Sciences, Wuhan University, Wuhan 430072, China; Corresponding authorSummary: Tumor-derived small extracellular vesicles (TEVs) play a pivotal role in cancer progression by transferring functional biomolecules between the parental and recipient cells. Here, we present a protocol to isolate TEVs directly from murine primary mammary tumor using differential centrifugation. We describe steps for tissue dissociation, enzymatic digestion, and centrifugation. We then detail procedures for characterization of TEVs through transmission electron microscopy, immunoblotting, and nano-flow cytometry. This protocol can be used to extract EVs from other solid tumor types.For complete details on the use and execution of this protocol, please refer to Li, Mei-Xin et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723007505Cell BiologyCell isolationCancer |
| spellingShingle | Sheng Hu Xiaohui Zhang Wei Yan Isolation and characterization of small extracellular vesicles from murine primary mammary tumor STAR Protocols Cell Biology Cell isolation Cancer |
| title | Isolation and characterization of small extracellular vesicles from murine primary mammary tumor |
| title_full | Isolation and characterization of small extracellular vesicles from murine primary mammary tumor |
| title_fullStr | Isolation and characterization of small extracellular vesicles from murine primary mammary tumor |
| title_full_unstemmed | Isolation and characterization of small extracellular vesicles from murine primary mammary tumor |
| title_short | Isolation and characterization of small extracellular vesicles from murine primary mammary tumor |
| title_sort | isolation and characterization of small extracellular vesicles from murine primary mammary tumor |
| topic | Cell Biology Cell isolation Cancer |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723007505 |
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