Current Status and Prospects of the Single-Cell Sequencing Technologies for Revealing the Pathogenesis of Pregnancy-Associated Disorders
Single-cell RNA sequencing (scRNA-seq) is a method that focuses on the analysis of gene expression profile in individual cells. This method has been successfully applied to answer the challenging questions of the pathogenesis of multifactorial diseases and open up new possibilities in the prognosis...
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MDPI AG
2023-03-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/3/756 |
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| author | Dmitry D. Naydenov Elena S. Vashukova Yury A. Barbitoff Yulia A. Nasykhova Andrey S. Glotov |
| author_facet | Dmitry D. Naydenov Elena S. Vashukova Yury A. Barbitoff Yulia A. Nasykhova Andrey S. Glotov |
| author_sort | Dmitry D. Naydenov |
| collection | DOAJ |
| description | Single-cell RNA sequencing (scRNA-seq) is a method that focuses on the analysis of gene expression profile in individual cells. This method has been successfully applied to answer the challenging questions of the pathogenesis of multifactorial diseases and open up new possibilities in the prognosis and prevention of reproductive diseases. In this article, we have reviewed the application of scRNA-seq to the analysis of the various cell types and their gene expression changes in normal pregnancy and pregnancy complications. The main principle, advantages, and limitations of single-cell technologies and data analysis methods are described. We discuss the possibilities of using the scRNA-seq method for solving the fundamental and applied tasks related to various pregnancy-associated disorders. Finally, we provide an overview of the scRNA-seq findings for the common pregnancy-associated conditions, such as hyperglycemia in pregnancy, recurrent pregnancy loss, preterm labor, polycystic ovary syndrome, and pre-eclampsia. |
| first_indexed | 2024-03-11T06:29:22Z |
| format | Article |
| id | doaj.art-af97135a7d1044d184a1709c9d8b6e11 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T06:29:22Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-af97135a7d1044d184a1709c9d8b6e112023-11-17T11:19:00ZengMDPI AGGenes2073-44252023-03-0114375610.3390/genes14030756Current Status and Prospects of the Single-Cell Sequencing Technologies for Revealing the Pathogenesis of Pregnancy-Associated DisordersDmitry D. Naydenov0Elena S. Vashukova1Yury A. Barbitoff2Yulia A. Nasykhova3Andrey S. Glotov4Faculty of Biology, St. Petersburg State University, 199034 Saint-Petersburg, RussiaD. O. Ott Research Institute of Obstetrics, Gynaecology and Reproductology, 199034 Saint-Petersburg, RussiaFaculty of Biology, St. Petersburg State University, 199034 Saint-Petersburg, RussiaD. O. Ott Research Institute of Obstetrics, Gynaecology and Reproductology, 199034 Saint-Petersburg, RussiaFaculty of Biology, St. Petersburg State University, 199034 Saint-Petersburg, RussiaSingle-cell RNA sequencing (scRNA-seq) is a method that focuses on the analysis of gene expression profile in individual cells. This method has been successfully applied to answer the challenging questions of the pathogenesis of multifactorial diseases and open up new possibilities in the prognosis and prevention of reproductive diseases. In this article, we have reviewed the application of scRNA-seq to the analysis of the various cell types and their gene expression changes in normal pregnancy and pregnancy complications. The main principle, advantages, and limitations of single-cell technologies and data analysis methods are described. We discuss the possibilities of using the scRNA-seq method for solving the fundamental and applied tasks related to various pregnancy-associated disorders. Finally, we provide an overview of the scRNA-seq findings for the common pregnancy-associated conditions, such as hyperglycemia in pregnancy, recurrent pregnancy loss, preterm labor, polycystic ovary syndrome, and pre-eclampsia.https://www.mdpi.com/2073-4425/14/3/756single-cell RNA sequencingbioinformaticspregnancy-associated disordersreproductive diseasespre-eclampsiapolycystic ovary syndrome |
| spellingShingle | Dmitry D. Naydenov Elena S. Vashukova Yury A. Barbitoff Yulia A. Nasykhova Andrey S. Glotov Current Status and Prospects of the Single-Cell Sequencing Technologies for Revealing the Pathogenesis of Pregnancy-Associated Disorders Genes single-cell RNA sequencing bioinformatics pregnancy-associated disorders reproductive diseases pre-eclampsia polycystic ovary syndrome |
| title | Current Status and Prospects of the Single-Cell Sequencing Technologies for Revealing the Pathogenesis of Pregnancy-Associated Disorders |
| title_full | Current Status and Prospects of the Single-Cell Sequencing Technologies for Revealing the Pathogenesis of Pregnancy-Associated Disorders |
| title_fullStr | Current Status and Prospects of the Single-Cell Sequencing Technologies for Revealing the Pathogenesis of Pregnancy-Associated Disorders |
| title_full_unstemmed | Current Status and Prospects of the Single-Cell Sequencing Technologies for Revealing the Pathogenesis of Pregnancy-Associated Disorders |
| title_short | Current Status and Prospects of the Single-Cell Sequencing Technologies for Revealing the Pathogenesis of Pregnancy-Associated Disorders |
| title_sort | current status and prospects of the single cell sequencing technologies for revealing the pathogenesis of pregnancy associated disorders |
| topic | single-cell RNA sequencing bioinformatics pregnancy-associated disorders reproductive diseases pre-eclampsia polycystic ovary syndrome |
| url | https://www.mdpi.com/2073-4425/14/3/756 |
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