Establishment of a novel experimental model of infected anal fistula in rat
Abstract Refractory Crohn's-like enterocutaneous fistula indicates the aggressive manifestation and lead to poor prognosis of patients. The development of multidisciplinary strategies for fistula administration largely subjects to the deficiency of animal model for disease remodeling and the un...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-06-01
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| Series: | Laboratory Animal Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s42826-022-00125-4 |
| _version_ | 1818205326756806656 |
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| author | Meng Zhao Aitong Wang Leisheng Zhang Hao Yu |
| author_facet | Meng Zhao Aitong Wang Leisheng Zhang Hao Yu |
| author_sort | Meng Zhao |
| collection | DOAJ |
| description | Abstract Refractory Crohn's-like enterocutaneous fistula indicates the aggressive manifestation and lead to poor prognosis of patients. The development of multidisciplinary strategies for fistula administration largely subjects to the deficiency of animal model for disease remodeling and the underlying pathogenic mechanism. For the purpose, infected anal fistula model was conducted by BLV single-core electrolytic aluminum combined with dextran sodium sulfate. Notably, the inflammatory granulation tissue and inflammatory cell infiltration in the perianal tissue were arised on day 7 of the model by utilizing the Hematoxylin–eosin staining. With the aid of magnetic resonance imaging and signals of high-brightness. We intuitively observed the thickening and edema appeared in the fistula wall, which collectively suggested the formation of a fistula in the perianal area of the rat. Distinguish from the current models of anal fistula modeling including the body surface of fistula, backside of fistula and drainage wire of fistula, our model revealed multifaceted advantages such as quicker generation, higher modeling rate, preferable stability, better consistency, cost-effective, and in particular, more convenient to mimic clinical manifestations of anal fistula. |
| first_indexed | 2024-12-12T03:55:22Z |
| format | Article |
| id | doaj.art-af9822f83f98452a8f58c08560b499d7 |
| institution | Directory Open Access Journal |
| issn | 2233-7660 |
| language | English |
| last_indexed | 2024-12-12T03:55:22Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Laboratory Animal Research |
| spelling | doaj.art-af9822f83f98452a8f58c08560b499d72022-12-22T00:39:16ZengBMCLaboratory Animal Research2233-76602022-06-013811410.1186/s42826-022-00125-4Establishment of a novel experimental model of infected anal fistula in ratMeng Zhao0Aitong Wang1Leisheng Zhang2Hao Yu3National Engineering Research Center of Cell Products, AmCellGene Engineering Co., LtdNational Engineering Research Center of Cell Products, AmCellGene Engineering Co., LtdInstitute of Stem Cells, Health-Biotech (Tianjin) Stem Cell Research Institute Co., Ltd.The Postdoctoral Research Station, School of Medicine, Nankai UniversityAbstract Refractory Crohn's-like enterocutaneous fistula indicates the aggressive manifestation and lead to poor prognosis of patients. The development of multidisciplinary strategies for fistula administration largely subjects to the deficiency of animal model for disease remodeling and the underlying pathogenic mechanism. For the purpose, infected anal fistula model was conducted by BLV single-core electrolytic aluminum combined with dextran sodium sulfate. Notably, the inflammatory granulation tissue and inflammatory cell infiltration in the perianal tissue were arised on day 7 of the model by utilizing the Hematoxylin–eosin staining. With the aid of magnetic resonance imaging and signals of high-brightness. We intuitively observed the thickening and edema appeared in the fistula wall, which collectively suggested the formation of a fistula in the perianal area of the rat. Distinguish from the current models of anal fistula modeling including the body surface of fistula, backside of fistula and drainage wire of fistula, our model revealed multifaceted advantages such as quicker generation, higher modeling rate, preferable stability, better consistency, cost-effective, and in particular, more convenient to mimic clinical manifestations of anal fistula.https://doi.org/10.1186/s42826-022-00125-4Anal fistula modelingDSSBLV-SCEARat model |
| spellingShingle | Meng Zhao Aitong Wang Leisheng Zhang Hao Yu Establishment of a novel experimental model of infected anal fistula in rat Laboratory Animal Research Anal fistula modeling DSS BLV-SCEA Rat model |
| title | Establishment of a novel experimental model of infected anal fistula in rat |
| title_full | Establishment of a novel experimental model of infected anal fistula in rat |
| title_fullStr | Establishment of a novel experimental model of infected anal fistula in rat |
| title_full_unstemmed | Establishment of a novel experimental model of infected anal fistula in rat |
| title_short | Establishment of a novel experimental model of infected anal fistula in rat |
| title_sort | establishment of a novel experimental model of infected anal fistula in rat |
| topic | Anal fistula modeling DSS BLV-SCEA Rat model |
| url | https://doi.org/10.1186/s42826-022-00125-4 |
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