The QKI-6 RNA binding protein localizes with the MBP mRNAs in stress granules of glial cells.
BACKGROUND: The quaking viable (qk(v)) mouse has several developmental defects that result in rapid tremors in the hind limbs. The qkI gene expresses three major alternatively spliced mRNAs (5, 6 and 7 kb) that encode the QKI-5, QKI-6 and QKI-7 RNA binding proteins that differ in their C-terminal 30...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2010-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2941464?pdf=render |
| _version_ | 1818989314126643200 |
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| author | Yunling Wang Geneviève Lacroix Jeffery Haines Evgueni Doukhanine Guillermina Almazan Stéphane Richard |
| author_facet | Yunling Wang Geneviève Lacroix Jeffery Haines Evgueni Doukhanine Guillermina Almazan Stéphane Richard |
| author_sort | Yunling Wang |
| collection | DOAJ |
| description | BACKGROUND: The quaking viable (qk(v)) mouse has several developmental defects that result in rapid tremors in the hind limbs. The qkI gene expresses three major alternatively spliced mRNAs (5, 6 and 7 kb) that encode the QKI-5, QKI-6 and QKI-7 RNA binding proteins that differ in their C-terminal 30 amino acids. The QKI isoforms are known to regulate RNA metabolism within oligodendrocytes, however, little is known about their roles during cellular stress. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we report an interaction between the QKI-6 isoform and a component of the RNA induced silencing complex (RISC), argonaute 2 (Ago2). We show in glial cells that QKI-6 co-localizes with Ago2 and the myelin basic protein mRNA in cytoplasmic stress granules. CONCLUSIONS: Our findings define the QKI isoforms as Ago2-interacting proteins. We also identify the QKI-6 isoform as a new component of stress granules in glial cells. |
| first_indexed | 2024-12-20T19:36:30Z |
| format | Article |
| id | doaj.art-af9f05d8070941ca9dfd6c7f01036983 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T19:36:30Z |
| publishDate | 2010-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-af9f05d8070941ca9dfd6c7f010369832022-12-21T19:28:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-01-01593307331410.1371/journal.pone.0012824The QKI-6 RNA binding protein localizes with the MBP mRNAs in stress granules of glial cells.Yunling WangGeneviève LacroixJeffery HainesEvgueni DoukhanineGuillermina AlmazanStéphane RichardBACKGROUND: The quaking viable (qk(v)) mouse has several developmental defects that result in rapid tremors in the hind limbs. The qkI gene expresses three major alternatively spliced mRNAs (5, 6 and 7 kb) that encode the QKI-5, QKI-6 and QKI-7 RNA binding proteins that differ in their C-terminal 30 amino acids. The QKI isoforms are known to regulate RNA metabolism within oligodendrocytes, however, little is known about their roles during cellular stress. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we report an interaction between the QKI-6 isoform and a component of the RNA induced silencing complex (RISC), argonaute 2 (Ago2). We show in glial cells that QKI-6 co-localizes with Ago2 and the myelin basic protein mRNA in cytoplasmic stress granules. CONCLUSIONS: Our findings define the QKI isoforms as Ago2-interacting proteins. We also identify the QKI-6 isoform as a new component of stress granules in glial cells.http://europepmc.org/articles/PMC2941464?pdf=render |
| spellingShingle | Yunling Wang Geneviève Lacroix Jeffery Haines Evgueni Doukhanine Guillermina Almazan Stéphane Richard The QKI-6 RNA binding protein localizes with the MBP mRNAs in stress granules of glial cells. PLoS ONE |
| title | The QKI-6 RNA binding protein localizes with the MBP mRNAs in stress granules of glial cells. |
| title_full | The QKI-6 RNA binding protein localizes with the MBP mRNAs in stress granules of glial cells. |
| title_fullStr | The QKI-6 RNA binding protein localizes with the MBP mRNAs in stress granules of glial cells. |
| title_full_unstemmed | The QKI-6 RNA binding protein localizes with the MBP mRNAs in stress granules of glial cells. |
| title_short | The QKI-6 RNA binding protein localizes with the MBP mRNAs in stress granules of glial cells. |
| title_sort | qki 6 rna binding protein localizes with the mbp mrnas in stress granules of glial cells |
| url | http://europepmc.org/articles/PMC2941464?pdf=render |
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