Development of a biosensor for detection of pleural mesothelioma cancer biomarker using surface imprinting.
Hyaluronan-linked protein 1 (HAPLN1) which has been shown to be highly expressed in malignant pleural mesotheliomas (MPM), was detected in serum using an electrochemical surface-imprinting method. First, the detection method was optimized using Bovine serum albumin (BSA) as a model protein to mimic...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3596364?pdf=render |
| _version_ | 1818961100948897792 |
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| author | Aabhas Mathur Steven Blais Chandra M V Goparaju Thomas Neubert Harvey Pass Kalle Levon |
| author_facet | Aabhas Mathur Steven Blais Chandra M V Goparaju Thomas Neubert Harvey Pass Kalle Levon |
| author_sort | Aabhas Mathur |
| collection | DOAJ |
| description | Hyaluronan-linked protein 1 (HAPLN1) which has been shown to be highly expressed in malignant pleural mesotheliomas (MPM), was detected in serum using an electrochemical surface-imprinting method. First, the detection method was optimized using Bovine serum albumin (BSA) as a model protein to mimic the optimal conditions required to imprint the similar molecular weight protein HAPLN1. BSA was imprinted on the gold electrode with hydroxyl terminated alkane thiols, which formed a self-assembled monolayer (SAM) around BSA. The analyte (BSA) was then washed away and its imprint (empty cavity with shape-memory) was used for detection of BSA in a solution, using electrochemical open-circuit potential method, namely potentiometry. Factors considered to optimize the conditions include incubation time, protein concentration, limit of detection and size of electrode. Matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was used to confirm selectivity of imprints. With the obtained imprinting control parameters, HAPLN1 was imprinted in duplicate and the detection of spiked HAPLN1 was successfully conducted in serum. |
| first_indexed | 2024-12-20T12:08:04Z |
| format | Article |
| id | doaj.art-af9f1ef8cb13405daa0470a22fc24be6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T12:08:04Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-af9f1ef8cb13405daa0470a22fc24be62022-12-21T19:41:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0183e5768110.1371/journal.pone.0057681Development of a biosensor for detection of pleural mesothelioma cancer biomarker using surface imprinting.Aabhas MathurSteven BlaisChandra M V GoparajuThomas NeubertHarvey PassKalle LevonHyaluronan-linked protein 1 (HAPLN1) which has been shown to be highly expressed in malignant pleural mesotheliomas (MPM), was detected in serum using an electrochemical surface-imprinting method. First, the detection method was optimized using Bovine serum albumin (BSA) as a model protein to mimic the optimal conditions required to imprint the similar molecular weight protein HAPLN1. BSA was imprinted on the gold electrode with hydroxyl terminated alkane thiols, which formed a self-assembled monolayer (SAM) around BSA. The analyte (BSA) was then washed away and its imprint (empty cavity with shape-memory) was used for detection of BSA in a solution, using electrochemical open-circuit potential method, namely potentiometry. Factors considered to optimize the conditions include incubation time, protein concentration, limit of detection and size of electrode. Matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was used to confirm selectivity of imprints. With the obtained imprinting control parameters, HAPLN1 was imprinted in duplicate and the detection of spiked HAPLN1 was successfully conducted in serum.http://europepmc.org/articles/PMC3596364?pdf=render |
| spellingShingle | Aabhas Mathur Steven Blais Chandra M V Goparaju Thomas Neubert Harvey Pass Kalle Levon Development of a biosensor for detection of pleural mesothelioma cancer biomarker using surface imprinting. PLoS ONE |
| title | Development of a biosensor for detection of pleural mesothelioma cancer biomarker using surface imprinting. |
| title_full | Development of a biosensor for detection of pleural mesothelioma cancer biomarker using surface imprinting. |
| title_fullStr | Development of a biosensor for detection of pleural mesothelioma cancer biomarker using surface imprinting. |
| title_full_unstemmed | Development of a biosensor for detection of pleural mesothelioma cancer biomarker using surface imprinting. |
| title_short | Development of a biosensor for detection of pleural mesothelioma cancer biomarker using surface imprinting. |
| title_sort | development of a biosensor for detection of pleural mesothelioma cancer biomarker using surface imprinting |
| url | http://europepmc.org/articles/PMC3596364?pdf=render |
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