Space and genealogy determine inter-individual differences in siderophore gene expression in bacterial colonies
Summary: Heterogeneity in gene expression is common among clonal cells in bacteria, although the sources and functions of variation often remain unknown. Here, we track cellular heterogeneity in the bacterium Pseudomonas aeruginosa during colony growth by focusing on siderophore gene expression (pyo...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-04-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124724004340 |
| _version_ | 1797203048826667008 |
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| author | Subham Mridha Tobias Wechsler Rolf Kümmerli |
| author_facet | Subham Mridha Tobias Wechsler Rolf Kümmerli |
| author_sort | Subham Mridha |
| collection | DOAJ |
| description | Summary: Heterogeneity in gene expression is common among clonal cells in bacteria, although the sources and functions of variation often remain unknown. Here, we track cellular heterogeneity in the bacterium Pseudomonas aeruginosa during colony growth by focusing on siderophore gene expression (pyoverdine versus pyochelin) important for iron nutrition. We find that the spatial position of cells within colonies and non-genetic yet heritable differences between cell lineages are significant sources of cellular heterogeneity, while cell pole age and lifespan have no effect. Regarding functions, our results indicate that cells adjust their siderophore investment strategies along a gradient from the colony center to its edge. Moreover, cell lineages with below-average siderophore investment benefit from lineages with above-average siderophore investment, presumably due to siderophore sharing. Our study highlights that single-cell experiments with dual gene expression reporters can identify sources of gene expression variation of interlinked traits and offer explanations for adaptive benefits in bacteria. |
| first_indexed | 2024-04-24T08:13:09Z |
| format | Article |
| id | doaj.art-afa076273f214cd8b5d9a92cd454e4c5 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-24T08:13:09Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-afa076273f214cd8b5d9a92cd454e4c52024-04-17T04:49:09ZengElsevierCell Reports2211-12472024-04-01434114106Space and genealogy determine inter-individual differences in siderophore gene expression in bacterial coloniesSubham Mridha0Tobias Wechsler1Rolf Kümmerli2Department of Quantitative Biomedicine, University of Zurich, 8057 Zurich, Switzerland; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Corresponding authorDepartment of Quantitative Biomedicine, University of Zurich, 8057 Zurich, SwitzerlandDepartment of Quantitative Biomedicine, University of Zurich, 8057 Zurich, Switzerland; Corresponding authorSummary: Heterogeneity in gene expression is common among clonal cells in bacteria, although the sources and functions of variation often remain unknown. Here, we track cellular heterogeneity in the bacterium Pseudomonas aeruginosa during colony growth by focusing on siderophore gene expression (pyoverdine versus pyochelin) important for iron nutrition. We find that the spatial position of cells within colonies and non-genetic yet heritable differences between cell lineages are significant sources of cellular heterogeneity, while cell pole age and lifespan have no effect. Regarding functions, our results indicate that cells adjust their siderophore investment strategies along a gradient from the colony center to its edge. Moreover, cell lineages with below-average siderophore investment benefit from lineages with above-average siderophore investment, presumably due to siderophore sharing. Our study highlights that single-cell experiments with dual gene expression reporters can identify sources of gene expression variation of interlinked traits and offer explanations for adaptive benefits in bacteria.http://www.sciencedirect.com/science/article/pii/S2211124724004340CP: Microbiology |
| spellingShingle | Subham Mridha Tobias Wechsler Rolf Kümmerli Space and genealogy determine inter-individual differences in siderophore gene expression in bacterial colonies Cell Reports CP: Microbiology |
| title | Space and genealogy determine inter-individual differences in siderophore gene expression in bacterial colonies |
| title_full | Space and genealogy determine inter-individual differences in siderophore gene expression in bacterial colonies |
| title_fullStr | Space and genealogy determine inter-individual differences in siderophore gene expression in bacterial colonies |
| title_full_unstemmed | Space and genealogy determine inter-individual differences in siderophore gene expression in bacterial colonies |
| title_short | Space and genealogy determine inter-individual differences in siderophore gene expression in bacterial colonies |
| title_sort | space and genealogy determine inter individual differences in siderophore gene expression in bacterial colonies |
| topic | CP: Microbiology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124724004340 |
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