Phenol-Grafted Alginate Sulfate Hydrogel as an Injectable FGF-2 Carrier
In the field of tissue engineering, fibroblast growth factor-2 (FGF-2) effectively regenerates damaged tissue and restores its biological function. However, FGF-2 readily diffuses and degrades under physiological conditions. Therefore, methods for the sustained and localized delivery of FGF-2 are ne...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Online Access: | https://www.mdpi.com/2310-2861/8/12/818 |
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| author | Ryota Goto Masaki Nakahata Shinji Sakai |
| author_facet | Ryota Goto Masaki Nakahata Shinji Sakai |
| author_sort | Ryota Goto |
| collection | DOAJ |
| description | In the field of tissue engineering, fibroblast growth factor-2 (FGF-2) effectively regenerates damaged tissue and restores its biological function. However, FGF-2 readily diffuses and degrades under physiological conditions. Therefore, methods for the sustained and localized delivery of FGF-2 are needed. Drug delivery systems using hydrogels as carriers have attracted significant interest. Injectable hydrogels with an affinity for FGF-2 are candidates for FGF-2 delivery systems. In this study, we fabricated a hydrogel from phenol-grafted alginate sulfate (AlgS-Ph) and investigated its application to the delivery of FGF-2. The hydrogel was prepared under mild conditions via horseradish peroxidase (HRP)-mediated cross-linking. Surface plasmon resonance (SPR) measurements show that the AlgS-Ph hydrogel has an affinity for FGF-2 in accordance with its degree of sulfation. Conditions for the preparation of the AlgS-Ph hydrogel, including HRP and H<sub>2</sub>O<sub>2</sub> concentrations, are optimized so that the hydrogel can be used as an injectable drug carrier. The hydrogel shows no cytotoxicity when using 10T1/2 cells as a model cell line. The angiogenesis assay shows that FGF-2 released from the AlgS-Ph hydrogel promotes the formation of blood vessels. These results indicate that the AlgS-Ph hydrogel is a suitable candidate for the FGF-2 carrier. |
| first_indexed | 2024-03-09T16:31:41Z |
| format | Article |
| id | doaj.art-afa2483e305143798969af1cf987fa23 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-09T16:31:41Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-afa2483e305143798969af1cf987fa232023-11-24T15:01:36ZengMDPI AGGels2310-28612022-12-0181281810.3390/gels8120818Phenol-Grafted Alginate Sulfate Hydrogel as an Injectable FGF-2 CarrierRyota Goto0Masaki Nakahata1Shinji Sakai2Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, Toyonaka 560-8531, JapanDepartment of Macromolecular Science, Graduate School of Science, Osaka University, Toyonaka 560-0043, JapanDepartment of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, Toyonaka 560-8531, JapanIn the field of tissue engineering, fibroblast growth factor-2 (FGF-2) effectively regenerates damaged tissue and restores its biological function. However, FGF-2 readily diffuses and degrades under physiological conditions. Therefore, methods for the sustained and localized delivery of FGF-2 are needed. Drug delivery systems using hydrogels as carriers have attracted significant interest. Injectable hydrogels with an affinity for FGF-2 are candidates for FGF-2 delivery systems. In this study, we fabricated a hydrogel from phenol-grafted alginate sulfate (AlgS-Ph) and investigated its application to the delivery of FGF-2. The hydrogel was prepared under mild conditions via horseradish peroxidase (HRP)-mediated cross-linking. Surface plasmon resonance (SPR) measurements show that the AlgS-Ph hydrogel has an affinity for FGF-2 in accordance with its degree of sulfation. Conditions for the preparation of the AlgS-Ph hydrogel, including HRP and H<sub>2</sub>O<sub>2</sub> concentrations, are optimized so that the hydrogel can be used as an injectable drug carrier. The hydrogel shows no cytotoxicity when using 10T1/2 cells as a model cell line. The angiogenesis assay shows that FGF-2 released from the AlgS-Ph hydrogel promotes the formation of blood vessels. These results indicate that the AlgS-Ph hydrogel is a suitable candidate for the FGF-2 carrier.https://www.mdpi.com/2310-2861/8/12/818FGF-2hydrogeltissue engineeringinjectabledrug delivery systemalginate |
| spellingShingle | Ryota Goto Masaki Nakahata Shinji Sakai Phenol-Grafted Alginate Sulfate Hydrogel as an Injectable FGF-2 Carrier Gels FGF-2 hydrogel tissue engineering injectable drug delivery system alginate |
| title | Phenol-Grafted Alginate Sulfate Hydrogel as an Injectable FGF-2 Carrier |
| title_full | Phenol-Grafted Alginate Sulfate Hydrogel as an Injectable FGF-2 Carrier |
| title_fullStr | Phenol-Grafted Alginate Sulfate Hydrogel as an Injectable FGF-2 Carrier |
| title_full_unstemmed | Phenol-Grafted Alginate Sulfate Hydrogel as an Injectable FGF-2 Carrier |
| title_short | Phenol-Grafted Alginate Sulfate Hydrogel as an Injectable FGF-2 Carrier |
| title_sort | phenol grafted alginate sulfate hydrogel as an injectable fgf 2 carrier |
| topic | FGF-2 hydrogel tissue engineering injectable drug delivery system alginate |
| url | https://www.mdpi.com/2310-2861/8/12/818 |
| work_keys_str_mv | AT ryotagoto phenolgraftedalginatesulfatehydrogelasaninjectablefgf2carrier AT masakinakahata phenolgraftedalginatesulfatehydrogelasaninjectablefgf2carrier AT shinjisakai phenolgraftedalginatesulfatehydrogelasaninjectablefgf2carrier |