Complex and non-redundant signals from individual odor receptors that underlie chemotaxis behavior in Drosophila melanogaster larvae
The rules by which odor receptors encode odors and allow behavior are still largely unexplored. Although large data sets of electrophysiological responses of receptors to odors have been generated, few hypotheses have been tested with behavioral assays. We use a data set on odor responses of Drosoph...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The Company of Biologists
2014-09-01
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| Series: | Biology Open |
| Subjects: | |
| Online Access: | http://bio.biologists.org/content/3/10/947 |
| _version_ | 1819131932836888576 |
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| author | Jeewanjot S. Grewal Christine Nguyen Raquel Robles Christina Cho Karolina Kir Nicole Fledderman George Gacharna Michael Wesolowski Christie Klinger Pedro Vallejo Lorien Menhennett Abhiram Nagaraj Chineze Ebo Garrett Peacy Eftihia Davelis David Kucher Sarah Giers Scott A. Kreher |
| author_facet | Jeewanjot S. Grewal Christine Nguyen Raquel Robles Christina Cho Karolina Kir Nicole Fledderman George Gacharna Michael Wesolowski Christie Klinger Pedro Vallejo Lorien Menhennett Abhiram Nagaraj Chineze Ebo Garrett Peacy Eftihia Davelis David Kucher Sarah Giers Scott A. Kreher |
| author_sort | Jeewanjot S. Grewal |
| collection | DOAJ |
| description | The rules by which odor receptors encode odors and allow behavior are still largely unexplored. Although large data sets of electrophysiological responses of receptors to odors have been generated, few hypotheses have been tested with behavioral assays. We use a data set on odor responses of Drosophila larval odor receptors coupled with chemotaxis behavioral assays to examine rules of odor coding. Using mutants of odor receptors, we have found that odor receptors with similar electrophysiological responses to odors across concentrations play non-redundant roles in odor coding at specific odor concentrations. We have also found that high affinity receptors for odors determine behavioral response thresholds, but the rules for determining peak behavioral responses are more complex. While receptor mutants typically show loss of attraction to odors, some receptor mutants result in increased attraction at specific odor concentrations. The odor receptor mutants were rescued using transgenic expression of odor receptors, validating assignment of phenotypes to the alleles. Vapor pressures alone cannot fully explain behavior in our assay. Finally, some odors that did not elicit strong electrophysiological responses are associated with behavioral phenotypes upon examination of odor receptor mutants. This result is consistent with the role of sensory neurons in lateral inhibition via local interneurons in the antennal lobe. Taken together, our results suggest a complexity of odor coding rules even in a simple olfactory sensory system. |
| first_indexed | 2024-12-22T09:23:22Z |
| format | Article |
| id | doaj.art-afa2d56314ab4ea895aa8efc6ff9d3d9 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-22T09:23:22Z |
| publishDate | 2014-09-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-afa2d56314ab4ea895aa8efc6ff9d3d92022-12-21T18:31:09ZengThe Company of BiologistsBiology Open2046-63902014-09-0131094795710.1242/bio.2014857320148573Complex and non-redundant signals from individual odor receptors that underlie chemotaxis behavior in Drosophila melanogaster larvaeJeewanjot S. GrewalChristine NguyenRaquel RoblesChristina ChoKarolina KirNicole FleddermanGeorge GacharnaMichael WesolowskiChristie KlingerPedro VallejoLorien MenhennettAbhiram NagarajChineze EboGarrett PeacyEftihia DavelisDavid KucherSarah GiersScott A. KreherThe rules by which odor receptors encode odors and allow behavior are still largely unexplored. Although large data sets of electrophysiological responses of receptors to odors have been generated, few hypotheses have been tested with behavioral assays. We use a data set on odor responses of Drosophila larval odor receptors coupled with chemotaxis behavioral assays to examine rules of odor coding. Using mutants of odor receptors, we have found that odor receptors with similar electrophysiological responses to odors across concentrations play non-redundant roles in odor coding at specific odor concentrations. We have also found that high affinity receptors for odors determine behavioral response thresholds, but the rules for determining peak behavioral responses are more complex. While receptor mutants typically show loss of attraction to odors, some receptor mutants result in increased attraction at specific odor concentrations. The odor receptor mutants were rescued using transgenic expression of odor receptors, validating assignment of phenotypes to the alleles. Vapor pressures alone cannot fully explain behavior in our assay. Finally, some odors that did not elicit strong electrophysiological responses are associated with behavioral phenotypes upon examination of odor receptor mutants. This result is consistent with the role of sensory neurons in lateral inhibition via local interneurons in the antennal lobe. Taken together, our results suggest a complexity of odor coding rules even in a simple olfactory sensory system.http://bio.biologists.org/content/3/10/947Odor receptorsOlfactionDrosophilaOr42aOr42b |
| spellingShingle | Jeewanjot S. Grewal Christine Nguyen Raquel Robles Christina Cho Karolina Kir Nicole Fledderman George Gacharna Michael Wesolowski Christie Klinger Pedro Vallejo Lorien Menhennett Abhiram Nagaraj Chineze Ebo Garrett Peacy Eftihia Davelis David Kucher Sarah Giers Scott A. Kreher Complex and non-redundant signals from individual odor receptors that underlie chemotaxis behavior in Drosophila melanogaster larvae Biology Open Odor receptors Olfaction Drosophila Or42a Or42b |
| title | Complex and non-redundant signals from individual odor receptors that underlie chemotaxis behavior in Drosophila melanogaster larvae |
| title_full | Complex and non-redundant signals from individual odor receptors that underlie chemotaxis behavior in Drosophila melanogaster larvae |
| title_fullStr | Complex and non-redundant signals from individual odor receptors that underlie chemotaxis behavior in Drosophila melanogaster larvae |
| title_full_unstemmed | Complex and non-redundant signals from individual odor receptors that underlie chemotaxis behavior in Drosophila melanogaster larvae |
| title_short | Complex and non-redundant signals from individual odor receptors that underlie chemotaxis behavior in Drosophila melanogaster larvae |
| title_sort | complex and non redundant signals from individual odor receptors that underlie chemotaxis behavior in drosophila melanogaster larvae |
| topic | Odor receptors Olfaction Drosophila Or42a Or42b |
| url | http://bio.biologists.org/content/3/10/947 |
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