Enhancing Activity of <i>Pleurotus sajor-caju</i> (Fr.) Sing β-1,3-Glucanoligosaccharide (<i>Ps</i>-GOS) on Proliferation, Differentiation, and Mineralization of MC3T3-E1 Cells through the Involvement of BMP-2/Runx2/MAPK/Wnt/β-Catenin Signaling Pathway
Osteoporosis is a leading world health problem that results from an imbalance between bone formation and bone resorption. β-glucans has been extensively reported to exhibit a wide range of biological activities, including antiosteoporosis both in vitro and in vivo. However, the molecular me...
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| Format: | Article |
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MDPI AG
2020-01-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/2/190 |
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| author | Thanintorn Yodthong Ureporn Kedjarune-Leggat Carl Smythe Pannawich Sukprasirt Aratee Aroonkesorn Rapepun Wititsuwannakul Thanawat Pitakpornpreecha |
| author_facet | Thanintorn Yodthong Ureporn Kedjarune-Leggat Carl Smythe Pannawich Sukprasirt Aratee Aroonkesorn Rapepun Wititsuwannakul Thanawat Pitakpornpreecha |
| author_sort | Thanintorn Yodthong |
| collection | DOAJ |
| description | Osteoporosis is a leading world health problem that results from an imbalance between bone formation and bone resorption. β-glucans has been extensively reported to exhibit a wide range of biological activities, including antiosteoporosis both in vitro and in vivo. However, the molecular mechanisms responsible for β-glucan-mediated bone formation in osteoblasts have not yet been investigated. The oyster mushroom <i>Pleurotus sajor-caju</i> produces abundant amounts of an insoluble β-glucan, which is rendered soluble by enzymatic degradation using <i>Hevea</i> glucanase to generate low-molecular-weight glucanoligosaccharide (<i>Ps</i>-GOS). This study aimed to investigate the osteogenic enhancing activity and underlining molecular mechanism of <i>Ps</i>-GOS on osteoblastogenesis of pre-osteoblastic MC3T3-E1 cells. In this study, it was demonstrated for the first time that low concentrations of <i>Ps</i>-GOS could promote cell proliferation and division after 48 h of treatment. In addition, <i>Ps</i>-GOS upregulated the mRNA and protein expression level of bone morphogenetic protein-2 (BMP-2) and runt-related transcription factor-2 (Runx2), which are both involved in BMP signaling pathway, accompanied by increased alkaline phosphatase (ALP) activity and mineralization. <i>Ps</i>-GOS also upregulated the expression of osteogenesis related genes including ALP, collagen type 1 (COL1), and osteocalcin (OCN). Moreover, our novel findings suggest that <i>Ps</i>-GOS may exert its effects through the mitogen-activated protein kinase (MAPK) and wingless-type MMTV integration site (Wnt)/β-catenin signaling pathways. |
| first_indexed | 2024-12-11T05:45:13Z |
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| id | doaj.art-afa9eb2fca4743759cfa5caef036a68b |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-11T05:45:13Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-afa9eb2fca4743759cfa5caef036a68b2022-12-22T01:19:00ZengMDPI AGBiomolecules2218-273X2020-01-0110219010.3390/biom10020190biom10020190Enhancing Activity of <i>Pleurotus sajor-caju</i> (Fr.) Sing β-1,3-Glucanoligosaccharide (<i>Ps</i>-GOS) on Proliferation, Differentiation, and Mineralization of MC3T3-E1 Cells through the Involvement of BMP-2/Runx2/MAPK/Wnt/β-Catenin Signaling PathwayThanintorn Yodthong0Ureporn Kedjarune-Leggat1Carl Smythe2Pannawich Sukprasirt3Aratee Aroonkesorn4Rapepun Wititsuwannakul5Thanawat Pitakpornpreecha6Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112, ThailandDepartment of Oral Biology and Occlusion, Faculty of Dentistry, Prince of Songkla University, Hat-Yai, Songkhla 90112, ThailandDepartment of Biomedical Science, University of Sheffield, Sheffield S10 2TN, UKCenter of Excellence in Natural Rubber Latex Biotechnology Research and Development, Prince of Songkla University, Hat-Yai, Songkhla 90112, ThailandDepartment of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112, ThailandCenter of Excellence in Natural Rubber Latex Biotechnology Research and Development, Prince of Songkla University, Hat-Yai, Songkhla 90112, ThailandDepartment of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112, ThailandOsteoporosis is a leading world health problem that results from an imbalance between bone formation and bone resorption. β-glucans has been extensively reported to exhibit a wide range of biological activities, including antiosteoporosis both in vitro and in vivo. However, the molecular mechanisms responsible for β-glucan-mediated bone formation in osteoblasts have not yet been investigated. The oyster mushroom <i>Pleurotus sajor-caju</i> produces abundant amounts of an insoluble β-glucan, which is rendered soluble by enzymatic degradation using <i>Hevea</i> glucanase to generate low-molecular-weight glucanoligosaccharide (<i>Ps</i>-GOS). This study aimed to investigate the osteogenic enhancing activity and underlining molecular mechanism of <i>Ps</i>-GOS on osteoblastogenesis of pre-osteoblastic MC3T3-E1 cells. In this study, it was demonstrated for the first time that low concentrations of <i>Ps</i>-GOS could promote cell proliferation and division after 48 h of treatment. In addition, <i>Ps</i>-GOS upregulated the mRNA and protein expression level of bone morphogenetic protein-2 (BMP-2) and runt-related transcription factor-2 (Runx2), which are both involved in BMP signaling pathway, accompanied by increased alkaline phosphatase (ALP) activity and mineralization. <i>Ps</i>-GOS also upregulated the expression of osteogenesis related genes including ALP, collagen type 1 (COL1), and osteocalcin (OCN). Moreover, our novel findings suggest that <i>Ps</i>-GOS may exert its effects through the mitogen-activated protein kinase (MAPK) and wingless-type MMTV integration site (Wnt)/β-catenin signaling pathways.https://www.mdpi.com/2218-273X/10/2/190glucanoligosaccharidepleurotus sajor-cajuosteoblastogenesisβ-1,3-glucanase |
| spellingShingle | Thanintorn Yodthong Ureporn Kedjarune-Leggat Carl Smythe Pannawich Sukprasirt Aratee Aroonkesorn Rapepun Wititsuwannakul Thanawat Pitakpornpreecha Enhancing Activity of <i>Pleurotus sajor-caju</i> (Fr.) Sing β-1,3-Glucanoligosaccharide (<i>Ps</i>-GOS) on Proliferation, Differentiation, and Mineralization of MC3T3-E1 Cells through the Involvement of BMP-2/Runx2/MAPK/Wnt/β-Catenin Signaling Pathway Biomolecules glucanoligosaccharide pleurotus sajor-caju osteoblastogenesis β-1,3-glucanase |
| title | Enhancing Activity of <i>Pleurotus sajor-caju</i> (Fr.) Sing β-1,3-Glucanoligosaccharide (<i>Ps</i>-GOS) on Proliferation, Differentiation, and Mineralization of MC3T3-E1 Cells through the Involvement of BMP-2/Runx2/MAPK/Wnt/β-Catenin Signaling Pathway |
| title_full | Enhancing Activity of <i>Pleurotus sajor-caju</i> (Fr.) Sing β-1,3-Glucanoligosaccharide (<i>Ps</i>-GOS) on Proliferation, Differentiation, and Mineralization of MC3T3-E1 Cells through the Involvement of BMP-2/Runx2/MAPK/Wnt/β-Catenin Signaling Pathway |
| title_fullStr | Enhancing Activity of <i>Pleurotus sajor-caju</i> (Fr.) Sing β-1,3-Glucanoligosaccharide (<i>Ps</i>-GOS) on Proliferation, Differentiation, and Mineralization of MC3T3-E1 Cells through the Involvement of BMP-2/Runx2/MAPK/Wnt/β-Catenin Signaling Pathway |
| title_full_unstemmed | Enhancing Activity of <i>Pleurotus sajor-caju</i> (Fr.) Sing β-1,3-Glucanoligosaccharide (<i>Ps</i>-GOS) on Proliferation, Differentiation, and Mineralization of MC3T3-E1 Cells through the Involvement of BMP-2/Runx2/MAPK/Wnt/β-Catenin Signaling Pathway |
| title_short | Enhancing Activity of <i>Pleurotus sajor-caju</i> (Fr.) Sing β-1,3-Glucanoligosaccharide (<i>Ps</i>-GOS) on Proliferation, Differentiation, and Mineralization of MC3T3-E1 Cells through the Involvement of BMP-2/Runx2/MAPK/Wnt/β-Catenin Signaling Pathway |
| title_sort | enhancing activity of i pleurotus sajor caju i fr sing β 1 3 glucanoligosaccharide i ps i gos on proliferation differentiation and mineralization of mc3t3 e1 cells through the involvement of bmp 2 runx2 mapk wnt β catenin signaling pathway |
| topic | glucanoligosaccharide pleurotus sajor-caju osteoblastogenesis β-1,3-glucanase |
| url | https://www.mdpi.com/2218-273X/10/2/190 |
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