| Summary: | The research aims to evaluate the influence of substrates and concentrations of indol butyric acid (AIB) on the vegetative propagation of Cinchona officinalis L. (quina) in Amazonas, Peru. A complete randomized factorial design of 3A x 4B was used, where factor A: substrates (sand, sand 50 % + peat 50 % and peat) and factor B: AIB concentration (0, 1, 2, 3 g.L-1). The study was developed in two phases. In the field, juvenile orthotropic branches of plus trees were collected from the San Jerónimo cloud forest in Peru, located at 2616 meters above sea level. The nursery phase was developed in the experimental center of the National University Toribio Rodriguez of Mendoza-Amazon. The juvenile branches collected from the field were uniformed to 7 cm, leaving two leaves with 50 % of area, were disinfected with Propineb 70 % fungicide 3 g.L-1 of water. It was found that substrates influenced significantly, standing out the sand substrate in the survival and sand + peat showed superiority in rooting, number and length of roots. Concentrations of 2 and 3 g.L-1 of AIB, stimulated root emission and increased the number and size of roots. Substrates and AIB concentrations positively influenced the rooting of young branches of C. officinalis under controlled environmental conditions.
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