Plasma Extracellular Vesicles-Derived miR-99a-5p: A Potential Biomarker to Predict Early Head and Neck Squamous Cell Carcinoma
Purpose: This study aimed to investigate the applicability of plasma extracellular vesicles (EVs) miR-99a-5p as a potential head and neck squamous cell carcinoma (HNSCC) diagnostic biomarker.Methods: The miRNA expression of HNSCC tissue and plasma EVs were profiled by small RNA sequencing. qRT-PCR w...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Pathology and Oncology Research |
| Subjects: | |
| Online Access: | https://www.por-journal.com/articles/10.3389/pore.2022.1610699/full |
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| author | Qiang Huang Qiang Huang Yu-Jie Shen Yu-Jie Shen Chi-Yao Hsueh Chi-Yao Hsueh Yi-Fan Zhang Yi-Fan Zhang Xiao-Hui Yuan Xiao-Hui Yuan Yu-Juan Zhou Yu-Juan Zhou Jiao-Yu Li Lan Lin Chun-Ping Wu Chun-Ping Wu Chun-Yan Hu |
| author_facet | Qiang Huang Qiang Huang Yu-Jie Shen Yu-Jie Shen Chi-Yao Hsueh Chi-Yao Hsueh Yi-Fan Zhang Yi-Fan Zhang Xiao-Hui Yuan Xiao-Hui Yuan Yu-Juan Zhou Yu-Juan Zhou Jiao-Yu Li Lan Lin Chun-Ping Wu Chun-Ping Wu Chun-Yan Hu |
| author_sort | Qiang Huang |
| collection | DOAJ |
| description | Purpose: This study aimed to investigate the applicability of plasma extracellular vesicles (EVs) miR-99a-5p as a potential head and neck squamous cell carcinoma (HNSCC) diagnostic biomarker.Methods: The miRNA expression of HNSCC tissue and plasma EVs were profiled by small RNA sequencing. qRT-PCR was performed to detect miR-99a-5p expression in HNSCC (n = 93) and benign disease (n = 39) plasma EVs and formalin-fixed and paraffin-embedded (FFPE) tissue (n = 110). We constructed receiver-operating characteristic curves to investigate the diagnostic efficiency of plasma EVs miR-99a-5p.Results: Tumor tissue exhibited lower miR-99a-5p than para-tumor tissue. Patients with high miR-99a-5p expression exhibited significantly more p16 positive status. In contrast, HNSCC plasma EVs harbored more miR-99a-5p than the benign disease group. Plasma EVs miR-99a-5p distinguished HNSCC with area under the curve (AUC) of 0.7494 (95% CI: 0.6692–0.8296; p < 0.0001), with 61.54% sensitivity and 75.27% specificity, respectively. Furthermore, plasma EVs miR-99a-5p also distinguished early HNSCC with AUC of 0.7394 (95% CI: 0.6284–0.8504; p = 0.0002), with 79.07% sensitivity and 61.54% specificity, respectively.Conclusion: Plasma EVs miR-99a-5p is a potential biomarker for predicting early HNSCC. |
| first_indexed | 2024-04-24T12:13:52Z |
| format | Article |
| id | doaj.art-afae19af314e4e08a9cf2b0726bdeaf2 |
| institution | Directory Open Access Journal |
| issn | 1532-2807 |
| language | English |
| last_indexed | 2024-04-24T12:13:52Z |
| publishDate | 2022-10-01 |
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| series | Pathology and Oncology Research |
| spelling | doaj.art-afae19af314e4e08a9cf2b0726bdeaf22024-04-08T09:32:18ZengFrontiers Media S.A.Pathology and Oncology Research1532-28072022-10-012810.3389/pore.2022.16106991610699Plasma Extracellular Vesicles-Derived miR-99a-5p: A Potential Biomarker to Predict Early Head and Neck Squamous Cell CarcinomaQiang Huang0Qiang Huang1Yu-Jie Shen2Yu-Jie Shen3Chi-Yao Hsueh4Chi-Yao Hsueh5Yi-Fan Zhang6Yi-Fan Zhang7Xiao-Hui Yuan8Xiao-Hui Yuan9Yu-Juan Zhou10Yu-Juan Zhou11Jiao-Yu Li12Lan Lin13Chun-Ping Wu14Chun-Ping Wu15Chun-Yan Hu16Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, ChinaShanghai Key Clinical Disciplines of Otorhinolaryngology, Shanghai, ChinaDepartment of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, ChinaShanghai Key Clinical Disciplines of Otorhinolaryngology, Shanghai, ChinaDepartment of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, ChinaShanghai Key Clinical Disciplines of Otorhinolaryngology, Shanghai, ChinaDepartment of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, ChinaShanghai Key Clinical Disciplines of Otorhinolaryngology, Shanghai, ChinaDepartment of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, ChinaShanghai Key Clinical Disciplines of Otorhinolaryngology, Shanghai, ChinaDepartment of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, ChinaShanghai Key Clinical Disciplines of Otorhinolaryngology, Shanghai, ChinaDepartment of Pediatric, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Pathology, Eye & ENT Hospital, Fudan University, Shanghai, ChinaDepartment of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, ChinaShanghai Key Clinical Disciplines of Otorhinolaryngology, Shanghai, ChinaDepartment of Pathology, Eye & ENT Hospital, Fudan University, Shanghai, ChinaPurpose: This study aimed to investigate the applicability of plasma extracellular vesicles (EVs) miR-99a-5p as a potential head and neck squamous cell carcinoma (HNSCC) diagnostic biomarker.Methods: The miRNA expression of HNSCC tissue and plasma EVs were profiled by small RNA sequencing. qRT-PCR was performed to detect miR-99a-5p expression in HNSCC (n = 93) and benign disease (n = 39) plasma EVs and formalin-fixed and paraffin-embedded (FFPE) tissue (n = 110). We constructed receiver-operating characteristic curves to investigate the diagnostic efficiency of plasma EVs miR-99a-5p.Results: Tumor tissue exhibited lower miR-99a-5p than para-tumor tissue. Patients with high miR-99a-5p expression exhibited significantly more p16 positive status. In contrast, HNSCC plasma EVs harbored more miR-99a-5p than the benign disease group. Plasma EVs miR-99a-5p distinguished HNSCC with area under the curve (AUC) of 0.7494 (95% CI: 0.6692–0.8296; p < 0.0001), with 61.54% sensitivity and 75.27% specificity, respectively. Furthermore, plasma EVs miR-99a-5p also distinguished early HNSCC with AUC of 0.7394 (95% CI: 0.6284–0.8504; p = 0.0002), with 79.07% sensitivity and 61.54% specificity, respectively.Conclusion: Plasma EVs miR-99a-5p is a potential biomarker for predicting early HNSCC.https://www.por-journal.com/articles/10.3389/pore.2022.1610699/fullbiomarkerHNSCCHPVplasma extracellular vesiclesmiR-99a-5p |
| spellingShingle | Qiang Huang Qiang Huang Yu-Jie Shen Yu-Jie Shen Chi-Yao Hsueh Chi-Yao Hsueh Yi-Fan Zhang Yi-Fan Zhang Xiao-Hui Yuan Xiao-Hui Yuan Yu-Juan Zhou Yu-Juan Zhou Jiao-Yu Li Lan Lin Chun-Ping Wu Chun-Ping Wu Chun-Yan Hu Plasma Extracellular Vesicles-Derived miR-99a-5p: A Potential Biomarker to Predict Early Head and Neck Squamous Cell Carcinoma Pathology and Oncology Research biomarker HNSCC HPV plasma extracellular vesicles miR-99a-5p |
| title | Plasma Extracellular Vesicles-Derived miR-99a-5p: A Potential Biomarker to Predict Early Head and Neck Squamous Cell Carcinoma |
| title_full | Plasma Extracellular Vesicles-Derived miR-99a-5p: A Potential Biomarker to Predict Early Head and Neck Squamous Cell Carcinoma |
| title_fullStr | Plasma Extracellular Vesicles-Derived miR-99a-5p: A Potential Biomarker to Predict Early Head and Neck Squamous Cell Carcinoma |
| title_full_unstemmed | Plasma Extracellular Vesicles-Derived miR-99a-5p: A Potential Biomarker to Predict Early Head and Neck Squamous Cell Carcinoma |
| title_short | Plasma Extracellular Vesicles-Derived miR-99a-5p: A Potential Biomarker to Predict Early Head and Neck Squamous Cell Carcinoma |
| title_sort | plasma extracellular vesicles derived mir 99a 5p a potential biomarker to predict early head and neck squamous cell carcinoma |
| topic | biomarker HNSCC HPV plasma extracellular vesicles miR-99a-5p |
| url | https://www.por-journal.com/articles/10.3389/pore.2022.1610699/full |
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