Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha.
We previously reported Agrobacterium-mediated transformation methods for the liverwort Marchantia polymorpha using the hygromycin phosphotransferase gene as a marker for selection with hygromycin. In this study, we developed three additional markers for M. polymorpha transformation: the gentamicin 3...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4583185?pdf=render |
| _version_ | 1811209624621154304 |
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| author | Kimitsune Ishizaki Ryuichi Nishihama Minoru Ueda Keisuke Inoue Sakiko Ishida Yoshiki Nishimura Toshiharu Shikanai Takayuki Kohchi |
| author_facet | Kimitsune Ishizaki Ryuichi Nishihama Minoru Ueda Keisuke Inoue Sakiko Ishida Yoshiki Nishimura Toshiharu Shikanai Takayuki Kohchi |
| author_sort | Kimitsune Ishizaki |
| collection | DOAJ |
| description | We previously reported Agrobacterium-mediated transformation methods for the liverwort Marchantia polymorpha using the hygromycin phosphotransferase gene as a marker for selection with hygromycin. In this study, we developed three additional markers for M. polymorpha transformation: the gentamicin 3'-acetyltransferase gene for selection with gentamicin; a mutated acetolactate synthase gene for selection with chlorsulfuron; and the neomycin phosphotransferase II gene for selection with G418. Based on these four marker genes, we have constructed a series of Gateway binary vectors designed for transgenic experiments on M. polymorpha. The 35S promoter from cauliflower mosaic virus and endogenous promoters for constitutive and heat-inducible expression were used to create these vectors. The reporters and tags used were Citrine, 3×Citrine, Citrine-NLS, TagRFP, tdTomato, tdTomato-NLS, GR, SRDX, SRDX-GR, GUS, ELuc(PEST), and 3×FLAG. These vectors, designated as the pMpGWB series, will facilitate molecular genetic analyses of the emerging model plant M. polymorpha. |
| first_indexed | 2024-04-12T04:42:26Z |
| format | Article |
| id | doaj.art-afb18b5c9e4f40379d17f0b4aa09e390 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T04:42:26Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-afb18b5c9e4f40379d17f0b4aa09e3902022-12-22T03:47:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01109e013887610.1371/journal.pone.0138876Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha.Kimitsune IshizakiRyuichi NishihamaMinoru UedaKeisuke InoueSakiko IshidaYoshiki NishimuraToshiharu ShikanaiTakayuki KohchiWe previously reported Agrobacterium-mediated transformation methods for the liverwort Marchantia polymorpha using the hygromycin phosphotransferase gene as a marker for selection with hygromycin. In this study, we developed three additional markers for M. polymorpha transformation: the gentamicin 3'-acetyltransferase gene for selection with gentamicin; a mutated acetolactate synthase gene for selection with chlorsulfuron; and the neomycin phosphotransferase II gene for selection with G418. Based on these four marker genes, we have constructed a series of Gateway binary vectors designed for transgenic experiments on M. polymorpha. The 35S promoter from cauliflower mosaic virus and endogenous promoters for constitutive and heat-inducible expression were used to create these vectors. The reporters and tags used were Citrine, 3×Citrine, Citrine-NLS, TagRFP, tdTomato, tdTomato-NLS, GR, SRDX, SRDX-GR, GUS, ELuc(PEST), and 3×FLAG. These vectors, designated as the pMpGWB series, will facilitate molecular genetic analyses of the emerging model plant M. polymorpha.http://europepmc.org/articles/PMC4583185?pdf=render |
| spellingShingle | Kimitsune Ishizaki Ryuichi Nishihama Minoru Ueda Keisuke Inoue Sakiko Ishida Yoshiki Nishimura Toshiharu Shikanai Takayuki Kohchi Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha. PLoS ONE |
| title | Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha. |
| title_full | Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha. |
| title_fullStr | Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha. |
| title_full_unstemmed | Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha. |
| title_short | Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha. |
| title_sort | development of gateway binary vector series with four different selection markers for the liverwort marchantia polymorpha |
| url | http://europepmc.org/articles/PMC4583185?pdf=render |
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