Activated membrane patches guide chemotactic cell motility.
Many eukaryotic cells are able to crawl on surfaces and guide their motility based on environmental cues. These cues are interpreted by signaling systems which couple to cell mechanics; indeed membrane protrusions in crawling cells are often accompanied by activated membrane patches, which are local...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-06-01
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| Series: | PLoS Computational Biology |
| Online Access: | http://europepmc.org/articles/PMC3127810?pdf=render |
| _version_ | 1818858411674042368 |
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| author | Inbal Hecht Monica L Skoge Pascale G Charest Eshel Ben-Jacob Richard A Firtel William F Loomis Herbert Levine Wouter-Jan Rappel |
| author_facet | Inbal Hecht Monica L Skoge Pascale G Charest Eshel Ben-Jacob Richard A Firtel William F Loomis Herbert Levine Wouter-Jan Rappel |
| author_sort | Inbal Hecht |
| collection | DOAJ |
| description | Many eukaryotic cells are able to crawl on surfaces and guide their motility based on environmental cues. These cues are interpreted by signaling systems which couple to cell mechanics; indeed membrane protrusions in crawling cells are often accompanied by activated membrane patches, which are localized areas of increased concentration of one or more signaling components. To determine how these patches are related to cell motion, we examine the spatial localization of RasGTP in chemotaxing Dictyostelium discoideum cells under conditions where the vertical extent of the cell was restricted. Quantitative analyses of the data reveal a high degree of spatial correlation between patches of activated Ras and membrane protrusions. Based on these findings, we formulate a model for amoeboid cell motion that consists of two coupled modules. The first module utilizes a recently developed two-component reaction diffusion model that generates transient and localized areas of elevated concentration of one of the components along the membrane. The activated patches determine the location of membrane protrusions (and overall cell motion) that are computed in the second module, which also takes into account the cortical tension and the availability of protrusion resources. We show that our model is able to produce realistic amoeboid-like motion and that our numerical results are consistent with experimentally observed pseudopod dynamics. Specifically, we show that the commonly observed splitting of pseudopods can result directly from the dynamics of the signaling patches. |
| first_indexed | 2024-12-19T08:55:49Z |
| format | Article |
| id | doaj.art-afb2ea48273a404fadbf0be2a0267721 |
| institution | Directory Open Access Journal |
| issn | 1553-734X 1553-7358 |
| language | English |
| last_indexed | 2024-12-19T08:55:49Z |
| publishDate | 2011-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Computational Biology |
| spelling | doaj.art-afb2ea48273a404fadbf0be2a02677212022-12-21T20:28:37ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582011-06-0176e100204410.1371/journal.pcbi.1002044Activated membrane patches guide chemotactic cell motility.Inbal HechtMonica L SkogePascale G CharestEshel Ben-JacobRichard A FirtelWilliam F LoomisHerbert LevineWouter-Jan RappelMany eukaryotic cells are able to crawl on surfaces and guide their motility based on environmental cues. These cues are interpreted by signaling systems which couple to cell mechanics; indeed membrane protrusions in crawling cells are often accompanied by activated membrane patches, which are localized areas of increased concentration of one or more signaling components. To determine how these patches are related to cell motion, we examine the spatial localization of RasGTP in chemotaxing Dictyostelium discoideum cells under conditions where the vertical extent of the cell was restricted. Quantitative analyses of the data reveal a high degree of spatial correlation between patches of activated Ras and membrane protrusions. Based on these findings, we formulate a model for amoeboid cell motion that consists of two coupled modules. The first module utilizes a recently developed two-component reaction diffusion model that generates transient and localized areas of elevated concentration of one of the components along the membrane. The activated patches determine the location of membrane protrusions (and overall cell motion) that are computed in the second module, which also takes into account the cortical tension and the availability of protrusion resources. We show that our model is able to produce realistic amoeboid-like motion and that our numerical results are consistent with experimentally observed pseudopod dynamics. Specifically, we show that the commonly observed splitting of pseudopods can result directly from the dynamics of the signaling patches.http://europepmc.org/articles/PMC3127810?pdf=render |
| spellingShingle | Inbal Hecht Monica L Skoge Pascale G Charest Eshel Ben-Jacob Richard A Firtel William F Loomis Herbert Levine Wouter-Jan Rappel Activated membrane patches guide chemotactic cell motility. PLoS Computational Biology |
| title | Activated membrane patches guide chemotactic cell motility. |
| title_full | Activated membrane patches guide chemotactic cell motility. |
| title_fullStr | Activated membrane patches guide chemotactic cell motility. |
| title_full_unstemmed | Activated membrane patches guide chemotactic cell motility. |
| title_short | Activated membrane patches guide chemotactic cell motility. |
| title_sort | activated membrane patches guide chemotactic cell motility |
| url | http://europepmc.org/articles/PMC3127810?pdf=render |
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