Effect of potassium intake on cadmium transporters and root cell wall biosynthesis in sweet potato
Large areas of farmland soil in southern China are deficient in potassium (K) and are contaminated with cadmium (Cd). Previously, we suggested that the K supplementation could reduce Cd accumulation in sweet potatoes (Ipomoea batatas (L.) Lam). In the present study, we investigated the underlying ph...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323000052 |
| _version_ | 1828064081616044032 |
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| author | Baifei Huang Qiong Liao Huiling Fu Ziyi Ye Yixiao Mao Jiemei Luo Yating Wang Haiwei Yuan Junliang Xin |
| author_facet | Baifei Huang Qiong Liao Huiling Fu Ziyi Ye Yixiao Mao Jiemei Luo Yating Wang Haiwei Yuan Junliang Xin |
| author_sort | Baifei Huang |
| collection | DOAJ |
| description | Large areas of farmland soil in southern China are deficient in potassium (K) and are contaminated with cadmium (Cd). Previously, we suggested that the K supplementation could reduce Cd accumulation in sweet potatoes (Ipomoea batatas (L.) Lam). In the present study, we investigated the underlying physiological and molecular mechanisms. A hydroponic experiment with different K and Cd treatments was performed to compare the transcriptome profile and the cell wall structure in the roots of sweet potato using RNA sequencing, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). The results showed that K supply inhibits the expressions of IRT1 and YSL3, which are responsible for root Cd uptake under Cd exposure. Furthermore, the expressions of COPT5 and Nramp3 were downregulated by K, which increased Cd retention in the root vacuoles. The upregulation of POD, CAD, INT1 and SUS by K contributed to lignin and cellulose biosynthesis and thickening of root xylem cell wall, which further reduced Cd translocation to the shoot. In addition, K affected the expressions of LHT, ACS, TPS and TPP associated with the production of ethylene and trehalose, which involved in plant resistance to Cd toxicity. In general, K application could decrease the uptake and translocation of Cd in sweet potatoes by regulating the expression of genes associated with Cd transporters and root cell wall components. |
| first_indexed | 2024-04-10T22:52:53Z |
| format | Article |
| id | doaj.art-afb5a7576998402983e4b3be553a39c8 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-10T22:52:53Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-afb5a7576998402983e4b3be553a39c82023-01-15T04:21:03ZengElsevierEcotoxicology and Environmental Safety0147-65132023-01-01250114501Effect of potassium intake on cadmium transporters and root cell wall biosynthesis in sweet potatoBaifei Huang0Qiong Liao1Huiling Fu2Ziyi Ye3Yixiao Mao4Jiemei Luo5Yating Wang6Haiwei Yuan7Junliang Xin8School of Chemical and Environmental Engineering, Hunan Institute of Technology, Hengyang 421002, ChinaSchool of Chemical and Environmental Engineering, Hunan Institute of Technology, Hengyang 421002, ChinaSchool of Chemical and Environmental Engineering, Hunan Institute of Technology, Hengyang 421002, ChinaSchool of Chemical and Environmental Engineering, Hunan Institute of Technology, Hengyang 421002, ChinaSchool of Chemical and Environmental Engineering, Hunan Institute of Technology, Hengyang 421002, ChinaSchool of Chemical and Environmental Engineering, Hunan Institute of Technology, Hengyang 421002, ChinaSchool of Chemical and Environmental Engineering, Hunan Institute of Technology, Hengyang 421002, ChinaHunan Huanbaoqiao Ecology and Environment Engineering Co., Ltd., Changsha 410221, ChinaSchool of Chemical and Environmental Engineering, Hunan Institute of Technology, Hengyang 421002, China; Corresponding author.Large areas of farmland soil in southern China are deficient in potassium (K) and are contaminated with cadmium (Cd). Previously, we suggested that the K supplementation could reduce Cd accumulation in sweet potatoes (Ipomoea batatas (L.) Lam). In the present study, we investigated the underlying physiological and molecular mechanisms. A hydroponic experiment with different K and Cd treatments was performed to compare the transcriptome profile and the cell wall structure in the roots of sweet potato using RNA sequencing, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). The results showed that K supply inhibits the expressions of IRT1 and YSL3, which are responsible for root Cd uptake under Cd exposure. Furthermore, the expressions of COPT5 and Nramp3 were downregulated by K, which increased Cd retention in the root vacuoles. The upregulation of POD, CAD, INT1 and SUS by K contributed to lignin and cellulose biosynthesis and thickening of root xylem cell wall, which further reduced Cd translocation to the shoot. In addition, K affected the expressions of LHT, ACS, TPS and TPP associated with the production of ethylene and trehalose, which involved in plant resistance to Cd toxicity. In general, K application could decrease the uptake and translocation of Cd in sweet potatoes by regulating the expression of genes associated with Cd transporters and root cell wall components.http://www.sciencedirect.com/science/article/pii/S0147651323000052BioaccumulationCadmium (Cd)Sweet potatoMacro-elementRNA-sequencingBiosynthesis |
| spellingShingle | Baifei Huang Qiong Liao Huiling Fu Ziyi Ye Yixiao Mao Jiemei Luo Yating Wang Haiwei Yuan Junliang Xin Effect of potassium intake on cadmium transporters and root cell wall biosynthesis in sweet potato Ecotoxicology and Environmental Safety Bioaccumulation Cadmium (Cd) Sweet potato Macro-element RNA-sequencing Biosynthesis |
| title | Effect of potassium intake on cadmium transporters and root cell wall biosynthesis in sweet potato |
| title_full | Effect of potassium intake on cadmium transporters and root cell wall biosynthesis in sweet potato |
| title_fullStr | Effect of potassium intake on cadmium transporters and root cell wall biosynthesis in sweet potato |
| title_full_unstemmed | Effect of potassium intake on cadmium transporters and root cell wall biosynthesis in sweet potato |
| title_short | Effect of potassium intake on cadmium transporters and root cell wall biosynthesis in sweet potato |
| title_sort | effect of potassium intake on cadmium transporters and root cell wall biosynthesis in sweet potato |
| topic | Bioaccumulation Cadmium (Cd) Sweet potato Macro-element RNA-sequencing Biosynthesis |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323000052 |
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