Development of fluorescent- and radio-traceable T1307-polymeric micelles as biomedical agents for cancer diagnosis: biodistribution on 4T1 tumor-bearing mice
Abstract In recent years, nanocarriers have been studied as promising pharmaceutical tools for controlled drug-delivery, treatment-efficacy follow-up and disease imaging. Among them, X-shaped amphiphilic polymeric micelles (Tetronic®, poloxamines) display great potential due to their biocompatibilit...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade de São Paulo
2022-07-01
|
| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100654&lng=en&tlng=en |
| _version_ | 1828270906810564608 |
|---|---|
| author | Nicole Lecot Gonzalo Rodríguez Valentina Stancov Marcelo Fernández Mercedes González Romina J. Glisoni Pablo Cabral Hugo Cerecetto |
| author_facet | Nicole Lecot Gonzalo Rodríguez Valentina Stancov Marcelo Fernández Mercedes González Romina J. Glisoni Pablo Cabral Hugo Cerecetto |
| author_sort | Nicole Lecot |
| collection | DOAJ |
| description | Abstract In recent years, nanocarriers have been studied as promising pharmaceutical tools for controlled drug-delivery, treatment-efficacy follow-up and disease imaging. Among them, X-shaped amphiphilic polymeric micelles (Tetronic®, poloxamines) display great potential due to their biocompatibility and non-toxic effects, among others. In the present work, polymeric micelles based on the T1307 copolymer were initially decorated with a 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY)-fluorophore in order to determinate its in vivo biodistribution on 4T1 tumor-bearing mice. However, unfavorable results with this probe led to two different strategies. On the one hand, the BODIPY-micelle-loaded, L-T1307-BODIPY, and on the other hand, the 99mTc-micelle-radiolabeled, L-T1307- 99m Tc, were analyzed separately in vivo. The results indicated that T1307 accumulates mainly in the stomach, the kidneys, the lungs and the tumor, reaching the maximum organ-accumulation 2 hours after intravenous injection. Additionally, and according to the results obtained for L-T1307- 99m Tc, the capture of the polymeric micelles in organs could be observed up to 24 hours after injection. The results obtained in this work were promising towards the development of new radiotracer agents for breast cancer based on X-shaped polymeric micelles. |
| first_indexed | 2024-04-13T05:48:01Z |
| format | Article |
| id | doaj.art-afbe30a77c4f4d8790569ce988c78676 |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-04-13T05:48:01Z |
| publishDate | 2022-07-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-afbe30a77c4f4d8790569ce988c786762022-12-22T02:59:54ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902022-07-015810.1590/s2175-97902022e191055Development of fluorescent- and radio-traceable T1307-polymeric micelles as biomedical agents for cancer diagnosis: biodistribution on 4T1 tumor-bearing miceNicole Lecothttps://orcid.org/0000-0003-2531-8026Gonzalo RodríguezValentina StancovMarcelo FernándezMercedes GonzálezRomina J. GlisoniPablo CabralHugo Cerecettohttps://orcid.org/0000-0003-1256-3786Abstract In recent years, nanocarriers have been studied as promising pharmaceutical tools for controlled drug-delivery, treatment-efficacy follow-up and disease imaging. Among them, X-shaped amphiphilic polymeric micelles (Tetronic®, poloxamines) display great potential due to their biocompatibility and non-toxic effects, among others. In the present work, polymeric micelles based on the T1307 copolymer were initially decorated with a 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY)-fluorophore in order to determinate its in vivo biodistribution on 4T1 tumor-bearing mice. However, unfavorable results with this probe led to two different strategies. On the one hand, the BODIPY-micelle-loaded, L-T1307-BODIPY, and on the other hand, the 99mTc-micelle-radiolabeled, L-T1307- 99m Tc, were analyzed separately in vivo. The results indicated that T1307 accumulates mainly in the stomach, the kidneys, the lungs and the tumor, reaching the maximum organ-accumulation 2 hours after intravenous injection. Additionally, and according to the results obtained for L-T1307- 99m Tc, the capture of the polymeric micelles in organs could be observed up to 24 hours after injection. The results obtained in this work were promising towards the development of new radiotracer agents for breast cancer based on X-shaped polymeric micelles.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100654&lng=en&tlng=enAmphiphilic polymeric micellesT1307BODIPY99mTcIn vivo biodistributionCancer diagnosis |
| spellingShingle | Nicole Lecot Gonzalo Rodríguez Valentina Stancov Marcelo Fernández Mercedes González Romina J. Glisoni Pablo Cabral Hugo Cerecetto Development of fluorescent- and radio-traceable T1307-polymeric micelles as biomedical agents for cancer diagnosis: biodistribution on 4T1 tumor-bearing mice Brazilian Journal of Pharmaceutical Sciences Amphiphilic polymeric micelles T1307 BODIPY 99mTc In vivo biodistribution Cancer diagnosis |
| title | Development of fluorescent- and radio-traceable T1307-polymeric micelles as biomedical agents for cancer diagnosis: biodistribution on 4T1 tumor-bearing mice |
| title_full | Development of fluorescent- and radio-traceable T1307-polymeric micelles as biomedical agents for cancer diagnosis: biodistribution on 4T1 tumor-bearing mice |
| title_fullStr | Development of fluorescent- and radio-traceable T1307-polymeric micelles as biomedical agents for cancer diagnosis: biodistribution on 4T1 tumor-bearing mice |
| title_full_unstemmed | Development of fluorescent- and radio-traceable T1307-polymeric micelles as biomedical agents for cancer diagnosis: biodistribution on 4T1 tumor-bearing mice |
| title_short | Development of fluorescent- and radio-traceable T1307-polymeric micelles as biomedical agents for cancer diagnosis: biodistribution on 4T1 tumor-bearing mice |
| title_sort | development of fluorescent and radio traceable t1307 polymeric micelles as biomedical agents for cancer diagnosis biodistribution on 4t1 tumor bearing mice |
| topic | Amphiphilic polymeric micelles T1307 BODIPY 99mTc In vivo biodistribution Cancer diagnosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100654&lng=en&tlng=en |
| work_keys_str_mv | AT nicolelecot developmentoffluorescentandradiotraceablet1307polymericmicellesasbiomedicalagentsforcancerdiagnosisbiodistributionon4t1tumorbearingmice AT gonzalorodriguez developmentoffluorescentandradiotraceablet1307polymericmicellesasbiomedicalagentsforcancerdiagnosisbiodistributionon4t1tumorbearingmice AT valentinastancov developmentoffluorescentandradiotraceablet1307polymericmicellesasbiomedicalagentsforcancerdiagnosisbiodistributionon4t1tumorbearingmice AT marcelofernandez developmentoffluorescentandradiotraceablet1307polymericmicellesasbiomedicalagentsforcancerdiagnosisbiodistributionon4t1tumorbearingmice AT mercedesgonzalez developmentoffluorescentandradiotraceablet1307polymericmicellesasbiomedicalagentsforcancerdiagnosisbiodistributionon4t1tumorbearingmice AT rominajglisoni developmentoffluorescentandradiotraceablet1307polymericmicellesasbiomedicalagentsforcancerdiagnosisbiodistributionon4t1tumorbearingmice AT pablocabral developmentoffluorescentandradiotraceablet1307polymericmicellesasbiomedicalagentsforcancerdiagnosisbiodistributionon4t1tumorbearingmice AT hugocerecetto developmentoffluorescentandradiotraceablet1307polymericmicellesasbiomedicalagentsforcancerdiagnosisbiodistributionon4t1tumorbearingmice |