Highly Efficient Cardiac Differentiation and Maintenance by Thrombin-Coagulated Fibrin Hydrogels Enriched with Decellularized Porcine Heart Extracellular Matrix
Biochemical and biophysical properties instruct cardiac tissue morphogenesis. Here, we are reporting on a blend of cardiac decellularized extracellular matrix (dECM) from porcine ventricular tissue and fibrinogen that is suitable for investigations employing an in vitro 3D cardiac cell culture model...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/3/2842 |
| _version_ | 1797624245074788352 |
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| author | Fatemeh Navaee Philippe Renaud Alexander Kleger Thomas Braschler |
| author_facet | Fatemeh Navaee Philippe Renaud Alexander Kleger Thomas Braschler |
| author_sort | Fatemeh Navaee |
| collection | DOAJ |
| description | Biochemical and biophysical properties instruct cardiac tissue morphogenesis. Here, we are reporting on a blend of cardiac decellularized extracellular matrix (dECM) from porcine ventricular tissue and fibrinogen that is suitable for investigations employing an in vitro 3D cardiac cell culture model. Rapid and specific coagulation with thrombin facilitates the gentle inclusion of cells while avoiding sedimentation during formation of the dECM-fibrin composite. Our investigations revealed enhanced cardiogenic differentiation in the H9c2 myoblast cells when using the system in a co-culture with Nor-10 fibroblasts. Further enhancement of differentiation efficiency was achieved by 3D embedding of rat neonatal cardiomyocytes in the 3D system. Calcium imaging and analysis of beating motion both indicate that the dECM-fibrin composite significantly enhances recovery, frequency, synchrony, and the maintenance of spontaneous beating, as compared to various controls including Matrigel, pure fibrin and collagen I as well as a fibrin-collagen I blend. |
| first_indexed | 2024-03-11T09:40:10Z |
| format | Article |
| id | doaj.art-afbeae87d7c140e1bc1539643180c1ad |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:40:10Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-afbeae87d7c140e1bc1539643180c1ad2023-11-16T17:03:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01243284210.3390/ijms24032842Highly Efficient Cardiac Differentiation and Maintenance by Thrombin-Coagulated Fibrin Hydrogels Enriched with Decellularized Porcine Heart Extracellular MatrixFatemeh Navaee0Philippe Renaud1Alexander Kleger2Thomas Braschler3Microsystems Laboratory-LMIS4, EPFL, 1015 Lausanne, SwitzerlandMicrosystems Laboratory-LMIS4, EPFL, 1015 Lausanne, SwitzerlandInstitute of Molecular Oncology and Stem Cell Biology, Ulm University Hospital, 89081 Ulm, GermanyDepartment of Pathology and Immunology, Faculty of Medicine, CMU, 1211 Geneva, SwitzerlandBiochemical and biophysical properties instruct cardiac tissue morphogenesis. Here, we are reporting on a blend of cardiac decellularized extracellular matrix (dECM) from porcine ventricular tissue and fibrinogen that is suitable for investigations employing an in vitro 3D cardiac cell culture model. Rapid and specific coagulation with thrombin facilitates the gentle inclusion of cells while avoiding sedimentation during formation of the dECM-fibrin composite. Our investigations revealed enhanced cardiogenic differentiation in the H9c2 myoblast cells when using the system in a co-culture with Nor-10 fibroblasts. Further enhancement of differentiation efficiency was achieved by 3D embedding of rat neonatal cardiomyocytes in the 3D system. Calcium imaging and analysis of beating motion both indicate that the dECM-fibrin composite significantly enhances recovery, frequency, synchrony, and the maintenance of spontaneous beating, as compared to various controls including Matrigel, pure fibrin and collagen I as well as a fibrin-collagen I blend.https://www.mdpi.com/1422-0067/24/3/2842dECM-fibrin hydrogelneonatal cardiomyocyte cultureH9c2 cell differentiationbeating synchrony3D co-culture |
| spellingShingle | Fatemeh Navaee Philippe Renaud Alexander Kleger Thomas Braschler Highly Efficient Cardiac Differentiation and Maintenance by Thrombin-Coagulated Fibrin Hydrogels Enriched with Decellularized Porcine Heart Extracellular Matrix International Journal of Molecular Sciences dECM-fibrin hydrogel neonatal cardiomyocyte culture H9c2 cell differentiation beating synchrony 3D co-culture |
| title | Highly Efficient Cardiac Differentiation and Maintenance by Thrombin-Coagulated Fibrin Hydrogels Enriched with Decellularized Porcine Heart Extracellular Matrix |
| title_full | Highly Efficient Cardiac Differentiation and Maintenance by Thrombin-Coagulated Fibrin Hydrogels Enriched with Decellularized Porcine Heart Extracellular Matrix |
| title_fullStr | Highly Efficient Cardiac Differentiation and Maintenance by Thrombin-Coagulated Fibrin Hydrogels Enriched with Decellularized Porcine Heart Extracellular Matrix |
| title_full_unstemmed | Highly Efficient Cardiac Differentiation and Maintenance by Thrombin-Coagulated Fibrin Hydrogels Enriched with Decellularized Porcine Heart Extracellular Matrix |
| title_short | Highly Efficient Cardiac Differentiation and Maintenance by Thrombin-Coagulated Fibrin Hydrogels Enriched with Decellularized Porcine Heart Extracellular Matrix |
| title_sort | highly efficient cardiac differentiation and maintenance by thrombin coagulated fibrin hydrogels enriched with decellularized porcine heart extracellular matrix |
| topic | dECM-fibrin hydrogel neonatal cardiomyocyte culture H9c2 cell differentiation beating synchrony 3D co-culture |
| url | https://www.mdpi.com/1422-0067/24/3/2842 |
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