Downregulation of TGF-β1 suppressed proliferation and increased chemosensitivity of ovarian cancer cells by promoting BRCA1/Smad3 signaling
Abstract Background Studies have demonstrated that transforming growth factor beta-1 (TGF-β1) exhibits oncogenic activity in different types of cancer, including ovarian cancer (OC). However, its regulatory mechanism in OC and whether TGF-β1 is involved in chemosensitivity regulation remains unclear...
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| Format: | Article |
| Language: | English |
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BMC
2018-12-01
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| Series: | Biological Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40659-018-0205-4 |
| _version_ | 1819160044187418624 |
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| author | Yanqiu Wang Jun Xiang Jianjun Wang Yazhong Ji |
| author_facet | Yanqiu Wang Jun Xiang Jianjun Wang Yazhong Ji |
| author_sort | Yanqiu Wang |
| collection | DOAJ |
| description | Abstract Background Studies have demonstrated that transforming growth factor beta-1 (TGF-β1) exhibits oncogenic activity in different types of cancer, including ovarian cancer (OC). However, its regulatory mechanism in OC and whether TGF-β1 is involved in chemosensitivity regulation remains unclear. Thus, the aim of this study was to investigate the role of TGF-β1 in OC. Methods The OC cell line SKOV3 was employed, and TGF-β1 overexpression or knockdown vectors were constructed. The cell proliferation of SKOV3 was evaluated with the cell counting kit (CCK8) kit after treatment with different concentrations of cis-platinum. Western blot and protein immunoprecipitation were employed to detect changes in BRCA1 and Smad3 expression and their interactions. Tumor growth in nude mice was evaluated. Results The results showed that TGF-β1 knockdown increased chemosensitivity by promoting BRCA1 expression and Smad3 phosphorylation. In vivo studies showed that TGF-β1 knockdown significantly inhibited the growth of tumors, also by upregulating BRCA1 expression and Smad3 phosphorylation. Conclusion Taken together, our results suggest that TGF-β1 knockdown inhibits tumor growth and increases chemosensitivity by promotion of BRCA1/Smad3 signaling. |
| first_indexed | 2024-12-22T16:50:11Z |
| format | Article |
| id | doaj.art-afbf2eb284ba4410a9c107021a86ba2c |
| institution | Directory Open Access Journal |
| issn | 0717-6287 |
| language | English |
| last_indexed | 2024-12-22T16:50:11Z |
| publishDate | 2018-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-afbf2eb284ba4410a9c107021a86ba2c2022-12-21T18:19:36ZengBMCBiological Research0717-62872018-12-015111710.1186/s40659-018-0205-4Downregulation of TGF-β1 suppressed proliferation and increased chemosensitivity of ovarian cancer cells by promoting BRCA1/Smad3 signalingYanqiu Wang0Jun Xiang1Jianjun Wang2Yazhong Ji3Reproductive Medical Center, Department of Gynecology and Obstetrics, Tongji Hospital, Tongji University School of MedicineReproductive Medical Center, Department of Gynecology and Obstetrics, Tongji Hospital, Tongji University School of MedicineDepartment of Gynecology and Obstetrics, Tongji Hospital, Tongji University School of MedicineReproductive Medical Center, Department of Gynecology and Obstetrics, Tongji Hospital, Tongji University School of MedicineAbstract Background Studies have demonstrated that transforming growth factor beta-1 (TGF-β1) exhibits oncogenic activity in different types of cancer, including ovarian cancer (OC). However, its regulatory mechanism in OC and whether TGF-β1 is involved in chemosensitivity regulation remains unclear. Thus, the aim of this study was to investigate the role of TGF-β1 in OC. Methods The OC cell line SKOV3 was employed, and TGF-β1 overexpression or knockdown vectors were constructed. The cell proliferation of SKOV3 was evaluated with the cell counting kit (CCK8) kit after treatment with different concentrations of cis-platinum. Western blot and protein immunoprecipitation were employed to detect changes in BRCA1 and Smad3 expression and their interactions. Tumor growth in nude mice was evaluated. Results The results showed that TGF-β1 knockdown increased chemosensitivity by promoting BRCA1 expression and Smad3 phosphorylation. In vivo studies showed that TGF-β1 knockdown significantly inhibited the growth of tumors, also by upregulating BRCA1 expression and Smad3 phosphorylation. Conclusion Taken together, our results suggest that TGF-β1 knockdown inhibits tumor growth and increases chemosensitivity by promotion of BRCA1/Smad3 signaling.http://link.springer.com/article/10.1186/s40659-018-0205-4Ovarian cancerTGF-β1BRCA1Smad3Proliferation |
| spellingShingle | Yanqiu Wang Jun Xiang Jianjun Wang Yazhong Ji Downregulation of TGF-β1 suppressed proliferation and increased chemosensitivity of ovarian cancer cells by promoting BRCA1/Smad3 signaling Biological Research Ovarian cancer TGF-β1 BRCA1 Smad3 Proliferation |
| title | Downregulation of TGF-β1 suppressed proliferation and increased chemosensitivity of ovarian cancer cells by promoting BRCA1/Smad3 signaling |
| title_full | Downregulation of TGF-β1 suppressed proliferation and increased chemosensitivity of ovarian cancer cells by promoting BRCA1/Smad3 signaling |
| title_fullStr | Downregulation of TGF-β1 suppressed proliferation and increased chemosensitivity of ovarian cancer cells by promoting BRCA1/Smad3 signaling |
| title_full_unstemmed | Downregulation of TGF-β1 suppressed proliferation and increased chemosensitivity of ovarian cancer cells by promoting BRCA1/Smad3 signaling |
| title_short | Downregulation of TGF-β1 suppressed proliferation and increased chemosensitivity of ovarian cancer cells by promoting BRCA1/Smad3 signaling |
| title_sort | downregulation of tgf β1 suppressed proliferation and increased chemosensitivity of ovarian cancer cells by promoting brca1 smad3 signaling |
| topic | Ovarian cancer TGF-β1 BRCA1 Smad3 Proliferation |
| url | http://link.springer.com/article/10.1186/s40659-018-0205-4 |
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