Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran
Background and Objective: Due to contagiousness of pertussis, a rapid and sensitive method for diagnosis is required to initiate the treatment and interrupt its transmission. Materials and Methods: To detect B. pertussis strains, we used two real-time PCR targeting IS481 and BP283 sequences and comp...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2013-09-01
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| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/595 |
| _version_ | 1818389533932126208 |
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| author | Vajiheh Sadat Nikbin Fereshteh Shahcheraghi Masoumeh Nakhost Lotfi Seyyed Mohsen Zahraei Masoumeh Parzadeh |
| author_facet | Vajiheh Sadat Nikbin Fereshteh Shahcheraghi Masoumeh Nakhost Lotfi Seyyed Mohsen Zahraei Masoumeh Parzadeh |
| author_sort | Vajiheh Sadat Nikbin |
| collection | DOAJ |
| description | Background and Objective: Due to contagiousness of pertussis, a rapid and sensitive method for diagnosis is required to initiate the treatment and interrupt its transmission.
Materials and Methods: To detect B. pertussis strains, we used two real-time PCR targeting IS481 and BP283 sequences and compared factors influencing culture and real-time PCR results.
Results: Totally, 779 specimens were collected from patients among which 11 (1.4%) were culture positive. Using IS481 and BP283 primers, 122 (15.6%) and 100 (12.8%) were diagnosed as infected specimens respectively. There were significant relationships between the real-time PCR method for diagnosis of B. pertussis and age, sex and vaccination of patients before sampling.
Conclusion: The real-time PCR is superior and much more sensitive than culture for diagnosis of B. pertussis. However, the sensitivity was improved when both IS481 and BP283 were used. Correct sampling and transportation of specimen also improved the detection rate in our research. |
| first_indexed | 2024-12-14T04:43:15Z |
| format | Article |
| id | doaj.art-afbfd46dff344a3b869c58171871bc91 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-12-14T04:43:15Z |
| publishDate | 2013-09-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-afbfd46dff344a3b869c58171871bc912022-12-21T23:16:45ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472013-09-0153Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in IranVajiheh Sadat Nikbin0Fereshteh Shahcheraghi1Masoumeh Nakhost Lotfi2Seyyed Mohsen Zahraei3Masoumeh Parzadeh4Pertussis Reference Laboratory, Department of Bacteriology, Pasteur Institute of Iran, Tehran.Pertussis Reference Laboratory, Department of Bacteriology, Pasteur Institute of Iran, Tehran.Pertussis Reference Laboratory, Department of Bacteriology, Pasteur Institute of Iran, Tehran.Center for Disease Control, Ministry of Health and Medical Education, Tehran, Iran.Pertussis Reference Laboratory, Department of Bacteriology, Pasteur Institute of Iran, Tehran.Background and Objective: Due to contagiousness of pertussis, a rapid and sensitive method for diagnosis is required to initiate the treatment and interrupt its transmission. Materials and Methods: To detect B. pertussis strains, we used two real-time PCR targeting IS481 and BP283 sequences and compared factors influencing culture and real-time PCR results. Results: Totally, 779 specimens were collected from patients among which 11 (1.4%) were culture positive. Using IS481 and BP283 primers, 122 (15.6%) and 100 (12.8%) were diagnosed as infected specimens respectively. There were significant relationships between the real-time PCR method for diagnosis of B. pertussis and age, sex and vaccination of patients before sampling. Conclusion: The real-time PCR is superior and much more sensitive than culture for diagnosis of B. pertussis. However, the sensitivity was improved when both IS481 and BP283 were used. Correct sampling and transportation of specimen also improved the detection rate in our research.https://ijm.tums.ac.ir/index.php/ijm/article/view/595BP283Bordetella pertussisIS481real-time PCR |
| spellingShingle | Vajiheh Sadat Nikbin Fereshteh Shahcheraghi Masoumeh Nakhost Lotfi Seyyed Mohsen Zahraei Masoumeh Parzadeh Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran Iranian Journal of Microbiology BP283 Bordetella pertussis IS481 real-time PCR |
| title | Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran |
| title_full | Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran |
| title_fullStr | Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran |
| title_full_unstemmed | Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran |
| title_short | Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran |
| title_sort | comparison of culture and real time pcr for detection of bordetella pertussis isolated from patients in iran |
| topic | BP283 Bordetella pertussis IS481 real-time PCR |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/595 |
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