Single-Cell Profiling of Kidney Transplant Recipients With Immunosuppressive Treatment Reveals the Dynamic Immune Characteristics
Kidney transplantation is currently the first choice of treatment for various types of end-stage renal failure, but there are major limitations in the application of immunosuppressive protocols after kidney transplantation. When the dose of immunosuppressant is too low, graft rejection occurs easily...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-04-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.639942/full |
| _version_ | 1818640805840027648 |
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| author | Yongguang Liu Xiaoyou Liu Song Zhou Ruiquan Xu Ruiquan Xu Jianmin Hu Guorong Liao Jun Liao Zefeng Guo Yuzhu Li Siqiang Yang Shichao Li Hua Chen Ying Guo Min Li Lipei Fan Liuyang Li Ming Zhao Ding Liu |
| author_facet | Yongguang Liu Xiaoyou Liu Song Zhou Ruiquan Xu Ruiquan Xu Jianmin Hu Guorong Liao Jun Liao Zefeng Guo Yuzhu Li Siqiang Yang Shichao Li Hua Chen Ying Guo Min Li Lipei Fan Liuyang Li Ming Zhao Ding Liu |
| author_sort | Yongguang Liu |
| collection | DOAJ |
| description | Kidney transplantation is currently the first choice of treatment for various types of end-stage renal failure, but there are major limitations in the application of immunosuppressive protocols after kidney transplantation. When the dose of immunosuppressant is too low, graft rejection occurs easily, while a dose that is too high can lead to graft loss. Therefore, it is very important to explore the immune status of patients receiving immunosuppressive agents after kidney transplantation. To compare the immune status of the recipient’s whole peripheral blood before and after receipt of immunosuppressive agents, we used single-cell cytometry by time-of-flight (CyTOF) to detect the peripheral blood immune cells in five kidney transplant recipients (KTRs) from the Department of Organ Transplantation of Zhujiang Hospital of Southern Medical University before and after receiving immunosuppressive agents. Based on CyTOF analysis, we detected 363,342 live single immune cells. We found that the immune cell types of the KTRs before and after receipt of immunosuppressive agents were mainly divided into CD4+ T cells, CD8+ T cells, B cells, NK cells/γδ T cells, monocytes/macrophages, granulocytes, and dendritic cells (DCs). After further reclustering of the above cell types, it was found that the immune cell subclusters in the peripheral blood of patients underwent major changes after receipt of immunosuppressants. After receiving immunosuppressive therapy, the peripheral blood of KTRs had significantly increased levels of CD57+NK cells and significantly decreased levels of central memory CD4+ T cells, follicular helper CD4+ T cells, effector CD8+ T cells, effector memory CD8+ T cells and naive CD8+ T cells. This study used CyTOF to classify immune cells in the peripheral blood of KTRs before and after immunosuppressive treatment, further compared differences in the proportions of the main immune cell types and immune cell subgroups before and after receipt of immunosuppressants, and provided relatively accurate information for assessment and treatment strategies for KTRs. |
| first_indexed | 2024-12-16T23:17:07Z |
| format | Article |
| id | doaj.art-afc094aec0524c758bc4e486fec3a52f |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-16T23:17:07Z |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-afc094aec0524c758bc4e486fec3a52f2022-12-21T22:12:16ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-04-011210.3389/fimmu.2021.639942639942Single-Cell Profiling of Kidney Transplant Recipients With Immunosuppressive Treatment Reveals the Dynamic Immune CharacteristicsYongguang Liu0Xiaoyou Liu1Song Zhou2Ruiquan Xu3Ruiquan Xu4Jianmin Hu5Guorong Liao6Jun Liao7Zefeng Guo8Yuzhu Li9Siqiang Yang10Shichao Li11Hua Chen12Ying Guo13Min Li14Lipei Fan15Liuyang Li16Ming Zhao17Ding Liu18Department of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Organ Transplantation, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaDepartment of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Urology, First Affiliated Hospital of Gannan Medical University, Ganzhou, ChinaDepartment of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaKidney transplantation is currently the first choice of treatment for various types of end-stage renal failure, but there are major limitations in the application of immunosuppressive protocols after kidney transplantation. When the dose of immunosuppressant is too low, graft rejection occurs easily, while a dose that is too high can lead to graft loss. Therefore, it is very important to explore the immune status of patients receiving immunosuppressive agents after kidney transplantation. To compare the immune status of the recipient’s whole peripheral blood before and after receipt of immunosuppressive agents, we used single-cell cytometry by time-of-flight (CyTOF) to detect the peripheral blood immune cells in five kidney transplant recipients (KTRs) from the Department of Organ Transplantation of Zhujiang Hospital of Southern Medical University before and after receiving immunosuppressive agents. Based on CyTOF analysis, we detected 363,342 live single immune cells. We found that the immune cell types of the KTRs before and after receipt of immunosuppressive agents were mainly divided into CD4+ T cells, CD8+ T cells, B cells, NK cells/γδ T cells, monocytes/macrophages, granulocytes, and dendritic cells (DCs). After further reclustering of the above cell types, it was found that the immune cell subclusters in the peripheral blood of patients underwent major changes after receipt of immunosuppressants. After receiving immunosuppressive therapy, the peripheral blood of KTRs had significantly increased levels of CD57+NK cells and significantly decreased levels of central memory CD4+ T cells, follicular helper CD4+ T cells, effector CD8+ T cells, effector memory CD8+ T cells and naive CD8+ T cells. This study used CyTOF to classify immune cells in the peripheral blood of KTRs before and after immunosuppressive treatment, further compared differences in the proportions of the main immune cell types and immune cell subgroups before and after receipt of immunosuppressants, and provided relatively accurate information for assessment and treatment strategies for KTRs.https://www.frontiersin.org/articles/10.3389/fimmu.2021.639942/fullkidney transplant recipientsCyTOFsingle-cell profilingimmune characteristicsimmunosuppressive treatment |
| spellingShingle | Yongguang Liu Xiaoyou Liu Song Zhou Ruiquan Xu Ruiquan Xu Jianmin Hu Guorong Liao Jun Liao Zefeng Guo Yuzhu Li Siqiang Yang Shichao Li Hua Chen Ying Guo Min Li Lipei Fan Liuyang Li Ming Zhao Ding Liu Single-Cell Profiling of Kidney Transplant Recipients With Immunosuppressive Treatment Reveals the Dynamic Immune Characteristics Frontiers in Immunology kidney transplant recipients CyTOF single-cell profiling immune characteristics immunosuppressive treatment |
| title | Single-Cell Profiling of Kidney Transplant Recipients With Immunosuppressive Treatment Reveals the Dynamic Immune Characteristics |
| title_full | Single-Cell Profiling of Kidney Transplant Recipients With Immunosuppressive Treatment Reveals the Dynamic Immune Characteristics |
| title_fullStr | Single-Cell Profiling of Kidney Transplant Recipients With Immunosuppressive Treatment Reveals the Dynamic Immune Characteristics |
| title_full_unstemmed | Single-Cell Profiling of Kidney Transplant Recipients With Immunosuppressive Treatment Reveals the Dynamic Immune Characteristics |
| title_short | Single-Cell Profiling of Kidney Transplant Recipients With Immunosuppressive Treatment Reveals the Dynamic Immune Characteristics |
| title_sort | single cell profiling of kidney transplant recipients with immunosuppressive treatment reveals the dynamic immune characteristics |
| topic | kidney transplant recipients CyTOF single-cell profiling immune characteristics immunosuppressive treatment |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.639942/full |
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