Fungal endophytes associated with Viola odorata Linn. as bioresource for pancreatic lipase inhibitors
Abstract Background As per the recent statistical reports of World Health Organisation (WHO), 13% of total global population is obese. Orlistat remains to be the only drug approved for the long term treatment of obesity. Recent findings highlighted severe adverse effects of orlistat that included he...
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| Format: | Article |
| Language: | English |
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BMC
2017-08-01
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| Series: | BMC Complementary and Alternative Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12906-017-1893-y |
| _version_ | 1818191055555657728 |
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| author | M. Katoch A. Paul G. Singh S. N. C. Sridhar |
| author_facet | M. Katoch A. Paul G. Singh S. N. C. Sridhar |
| author_sort | M. Katoch |
| collection | DOAJ |
| description | Abstract Background As per the recent statistical reports of World Health Organisation (WHO), 13% of total global population is obese. Orlistat remains to be the only drug approved for the long term treatment of obesity. Recent findings highlighted severe adverse effects of orlistat that included hepatotoxicity, gall stones, kidney stones and acute pancreatitis. Therefore, search for new drug is required. The investigations based on endophytic natural products would prove pivotal in the global fight against this health issue. Methods Obesity is associated with lipid metabolism involving pancreatic lipase enzyme. The inhibition of pancreatic lipase is demonstrated by using the extracts of endophytes isolated from Viola odorata Linn. In addition, endophytes were identified using ITS based rDNA sequencing. Results Present study involves the isolation and identification of 27 endophytes from V. odorata. All the endophytes were evaluated for lipase inhibitory activities. The extracts of seven endophytes exhibited lipase inhibitory activity with IC50 < 10 μg/mL. The extract of VOLF4 (Aspergillus sp.) displayed promising lipase inhibitory activity (IC50 3.8 μg/mL). Conclusion The present study demonstrates that V. odorata harbors endophytic community with potent lipase inhibitory activity. VOLF4 is the potential endophyte. The extract of VOLF4 can be used to develop the potential drug to treat obesity. |
| first_indexed | 2024-12-12T00:08:31Z |
| format | Article |
| id | doaj.art-afc5948fe3db441a9283dae06638548d |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-12T00:08:31Z |
| publishDate | 2017-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-afc5948fe3db441a9283dae06638548d2022-12-22T00:45:02ZengBMCBMC Complementary and Alternative Medicine1472-68822017-08-011711810.1186/s12906-017-1893-yFungal endophytes associated with Viola odorata Linn. as bioresource for pancreatic lipase inhibitorsM. Katoch0A. Paul1G. Singh2S. N. C. Sridhar3Department of Microbial Biotechnology, Indian Institute of Integrative MedicineLaboratory of Natural Drugs, Department of Pharmacy, Birla Institute of Technology and Science Pilani (Pilani campus)Department of Microbial Biotechnology, Indian Institute of Integrative MedicineLaboratory of Natural Drugs, Department of Pharmacy, Birla Institute of Technology and Science Pilani (Pilani campus)Abstract Background As per the recent statistical reports of World Health Organisation (WHO), 13% of total global population is obese. Orlistat remains to be the only drug approved for the long term treatment of obesity. Recent findings highlighted severe adverse effects of orlistat that included hepatotoxicity, gall stones, kidney stones and acute pancreatitis. Therefore, search for new drug is required. The investigations based on endophytic natural products would prove pivotal in the global fight against this health issue. Methods Obesity is associated with lipid metabolism involving pancreatic lipase enzyme. The inhibition of pancreatic lipase is demonstrated by using the extracts of endophytes isolated from Viola odorata Linn. In addition, endophytes were identified using ITS based rDNA sequencing. Results Present study involves the isolation and identification of 27 endophytes from V. odorata. All the endophytes were evaluated for lipase inhibitory activities. The extracts of seven endophytes exhibited lipase inhibitory activity with IC50 < 10 μg/mL. The extract of VOLF4 (Aspergillus sp.) displayed promising lipase inhibitory activity (IC50 3.8 μg/mL). Conclusion The present study demonstrates that V. odorata harbors endophytic community with potent lipase inhibitory activity. VOLF4 is the potential endophyte. The extract of VOLF4 can be used to develop the potential drug to treat obesity.http://link.springer.com/article/10.1186/s12906-017-1893-yEndophytic fungiViola odorataObesityPancreatic lipase inhibitory activityOrlistat |
| spellingShingle | M. Katoch A. Paul G. Singh S. N. C. Sridhar Fungal endophytes associated with Viola odorata Linn. as bioresource for pancreatic lipase inhibitors BMC Complementary and Alternative Medicine Endophytic fungi Viola odorata Obesity Pancreatic lipase inhibitory activity Orlistat |
| title | Fungal endophytes associated with Viola odorata Linn. as bioresource for pancreatic lipase inhibitors |
| title_full | Fungal endophytes associated with Viola odorata Linn. as bioresource for pancreatic lipase inhibitors |
| title_fullStr | Fungal endophytes associated with Viola odorata Linn. as bioresource for pancreatic lipase inhibitors |
| title_full_unstemmed | Fungal endophytes associated with Viola odorata Linn. as bioresource for pancreatic lipase inhibitors |
| title_short | Fungal endophytes associated with Viola odorata Linn. as bioresource for pancreatic lipase inhibitors |
| title_sort | fungal endophytes associated with viola odorata linn as bioresource for pancreatic lipase inhibitors |
| topic | Endophytic fungi Viola odorata Obesity Pancreatic lipase inhibitory activity Orlistat |
| url | http://link.springer.com/article/10.1186/s12906-017-1893-y |
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