Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration
Scaffolding biomaterials are gaining great importance due to their beneficial properties for medical purposes. Targeted biomaterial engineering strategies through the synergy of different material types can be applied to design hybrid scaffolding biomaterials with advantageous properties for biomedi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/15/3/589 |
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| author | Simonida Lj. Tomić Jovana S. Vuković Marija M. Babić Radić Vuk. V. Filipović Dubravka P. Živanović Miloš M. Nikolić Jasmina Nikodinovic-Runic |
| author_facet | Simonida Lj. Tomić Jovana S. Vuković Marija M. Babić Radić Vuk. V. Filipović Dubravka P. Živanović Miloš M. Nikolić Jasmina Nikodinovic-Runic |
| author_sort | Simonida Lj. Tomić |
| collection | DOAJ |
| description | Scaffolding biomaterials are gaining great importance due to their beneficial properties for medical purposes. Targeted biomaterial engineering strategies through the synergy of different material types can be applied to design hybrid scaffolding biomaterials with advantageous properties for biomedical applications. In our research, a novel combination of the bioactive agent Manuka honey (MHo) with 2-hydroxyethyl methacrylate/gelatin (HG) hydrogel scaffolds was created as an efficient bioactive platform for biomedical applications. The effects of Manuka honey content on structural characteristics, porosity, swelling performance, in vitro degradation, and in vitro biocompatibility (fibroblast and keratinocyte cell lines) of hybrid hydrogel scaffolds were studied using Fourier transform infrared spectroscopy, the gravimetric method, and in vitro MTT biocompatibility assays. The engineered hybrid hydrogel scaffolds show advantageous properties, including porosity in the range of 71.25% to 90.09%, specific pH- and temperature-dependent swelling performance, and convenient absorption capacity. In vitro degradation studies showed scaffold degradability ranging from 6.27% to 27.18% for four weeks. In vitro biocompatibility assays on healthy human fibroblast (MRC5 cells) and keratinocyte (HaCaT cells) cell lines by MTT tests showed that cell viability depends on the Manuka honey content loaded in the HG hydrogel scaffolds. A sample containing the highest Manuka honey content (30%) exhibited the best biocompatible properties. The obtained results reveal that the synergy of the bioactive agent, Manuka honey, with 2-hydroxyethyl methacrylate/gelatin as hybrid hydrogel scaffolds has potential for biomedical purposes. By tuning the Manuka honey content in HG hydrogel scaffolds advantageous properties of hybrid scaffolds can be achieved for biomedical applications. |
| first_indexed | 2024-03-11T09:28:43Z |
| format | Article |
| id | doaj.art-afc686f91c9a4ff781984a3df4e3a417 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-11T09:28:43Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-afc686f91c9a4ff781984a3df4e3a4172023-11-16T17:47:42ZengMDPI AGPolymers2073-43602023-01-0115358910.3390/polym15030589Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue RegenerationSimonida Lj. Tomić0Jovana S. Vuković1Marija M. Babić Radić2Vuk. V. Filipović3Dubravka P. Živanović4Miloš M. Nikolić5Jasmina Nikodinovic-Runic6University of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 Belgrade, SerbiaUniversity of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 Belgrade, SerbiaUniversity of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 Belgrade, SerbiaUniversity of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, 11000 Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, Department of Dermatology and Venereology, Pasterova 2, 11000 Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, Department of Dermatology and Venereology, Pasterova 2, 11000 Belgrade, SerbiaUniversity of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, 11000 Belgrade, SerbiaScaffolding biomaterials are gaining great importance due to their beneficial properties for medical purposes. Targeted biomaterial engineering strategies through the synergy of different material types can be applied to design hybrid scaffolding biomaterials with advantageous properties for biomedical applications. In our research, a novel combination of the bioactive agent Manuka honey (MHo) with 2-hydroxyethyl methacrylate/gelatin (HG) hydrogel scaffolds was created as an efficient bioactive platform for biomedical applications. The effects of Manuka honey content on structural characteristics, porosity, swelling performance, in vitro degradation, and in vitro biocompatibility (fibroblast and keratinocyte cell lines) of hybrid hydrogel scaffolds were studied using Fourier transform infrared spectroscopy, the gravimetric method, and in vitro MTT biocompatibility assays. The engineered hybrid hydrogel scaffolds show advantageous properties, including porosity in the range of 71.25% to 90.09%, specific pH- and temperature-dependent swelling performance, and convenient absorption capacity. In vitro degradation studies showed scaffold degradability ranging from 6.27% to 27.18% for four weeks. In vitro biocompatibility assays on healthy human fibroblast (MRC5 cells) and keratinocyte (HaCaT cells) cell lines by MTT tests showed that cell viability depends on the Manuka honey content loaded in the HG hydrogel scaffolds. A sample containing the highest Manuka honey content (30%) exhibited the best biocompatible properties. The obtained results reveal that the synergy of the bioactive agent, Manuka honey, with 2-hydroxyethyl methacrylate/gelatin as hybrid hydrogel scaffolds has potential for biomedical purposes. By tuning the Manuka honey content in HG hydrogel scaffolds advantageous properties of hybrid scaffolds can be achieved for biomedical applications.https://www.mdpi.com/2073-4360/15/3/589Manuka honey2-hydroxyethyl methacrylategelatinpH- and temperature-dependent swellingin vitro degradationin vitro biocompatibility |
| spellingShingle | Simonida Lj. Tomić Jovana S. Vuković Marija M. Babić Radić Vuk. V. Filipović Dubravka P. Živanović Miloš M. Nikolić Jasmina Nikodinovic-Runic Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration Polymers Manuka honey 2-hydroxyethyl methacrylate gelatin pH- and temperature-dependent swelling in vitro degradation in vitro biocompatibility |
| title | Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration |
| title_full | Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration |
| title_fullStr | Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration |
| title_full_unstemmed | Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration |
| title_short | Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration |
| title_sort | manuka honey 2 hydroxyethyl methacrylate gelatin hybrid hydrogel scaffolds for potential tissue regeneration |
| topic | Manuka honey 2-hydroxyethyl methacrylate gelatin pH- and temperature-dependent swelling in vitro degradation in vitro biocompatibility |
| url | https://www.mdpi.com/2073-4360/15/3/589 |
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