Acinetobacter baumannii outer membrane vesicles induced inflammation of human monocyte-derived macrophages THP-1
Objective To establish an ultra-filtration centrifugation method to purify the outer membrane vesicles (OMVs) of Acinetobacter baumannii(A.baumannii), and to investigate the ability of OMVs to induce inflamma-tion in human monocyte-derired macrophages THP-1. Methods A.baumannii was cultured, and the...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2023-01-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a210852.pdf |
| _version_ | 1797366377091170304 |
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| author | FU Shixiang, XI Yue, DING Longkun, YAN Man, ZHAO Jun, JIAO Yudong, WU Liang |
| author_facet | FU Shixiang, XI Yue, DING Longkun, YAN Man, ZHAO Jun, JIAO Yudong, WU Liang |
| author_sort | FU Shixiang, XI Yue, DING Longkun, YAN Man, ZHAO Jun, JIAO Yudong, WU Liang |
| collection | DOAJ |
| description | Objective To establish an ultra-filtration centrifugation method to purify the outer membrane vesicles (OMVs) of Acinetobacter baumannii(A.baumannii), and to investigate the ability of OMVs to induce inflamma-tion in human monocyte-derired macrophages THP-1. Methods A.baumannii was cultured, and the OMVs were purified from the supernatant of culture medium by ultra-filtration centrifuge,the morphology was observed by transmission electron microscopy. THP-1 cells were incubated with OMVs at the concentration of 5 and 50 μg/mL respectively. The expression of NOD-like receptor protein 3(NLRP3) and caspase-1 were determined by RT-qPCR. The concentration of IL-1β in culture medium supernatant was measured by ELISA. The expression of LC-3Ⅱ and Beclin-1 was examined by Western blot. The activity of caspase-3 was determined by enzyme dynamics assay. The expression of reactive oxygen species (ROS) was determined by flow cytometry. Results A.baumannii OMVs was purified successfully. The OMVs could activate NLRP3 inflammasome, increase the expression of LC-3Ⅱ and Beclin-1 and up-regulate the activity of caspase-3 (PPConclusions OMVs of A.baumannii can significantly activate macrophages NLRP3 inflammasome and induce cell autophagy and apoptosis. |
| first_indexed | 2024-03-08T17:03:17Z |
| format | Article |
| id | doaj.art-afc69c99fd334189adfc28566cf83106 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T17:03:17Z |
| publishDate | 2023-01-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-afc69c99fd334189adfc28566cf831062024-01-04T07:27:26ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252023-01-0143111011510.16352/j.issn.1001-6325.2023.01.0110Acinetobacter baumannii outer membrane vesicles induced inflammation of human monocyte-derived macrophages THP-1FU Shixiang, XI Yue, DING Longkun, YAN Man, ZHAO Jun, JIAO Yudong, WU Liang01. The Third People’s Hospital of Yangzhou, Yangzhou 225000;;2. School of Medicine, Jiangsu Uiversity, Zhenjiang 212013, ChinaObjective To establish an ultra-filtration centrifugation method to purify the outer membrane vesicles (OMVs) of Acinetobacter baumannii(A.baumannii), and to investigate the ability of OMVs to induce inflamma-tion in human monocyte-derired macrophages THP-1. Methods A.baumannii was cultured, and the OMVs were purified from the supernatant of culture medium by ultra-filtration centrifuge,the morphology was observed by transmission electron microscopy. THP-1 cells were incubated with OMVs at the concentration of 5 and 50 μg/mL respectively. The expression of NOD-like receptor protein 3(NLRP3) and caspase-1 were determined by RT-qPCR. The concentration of IL-1β in culture medium supernatant was measured by ELISA. The expression of LC-3Ⅱ and Beclin-1 was examined by Western blot. The activity of caspase-3 was determined by enzyme dynamics assay. The expression of reactive oxygen species (ROS) was determined by flow cytometry. Results A.baumannii OMVs was purified successfully. The OMVs could activate NLRP3 inflammasome, increase the expression of LC-3Ⅱ and Beclin-1 and up-regulate the activity of caspase-3 (PPConclusions OMVs of A.baumannii can significantly activate macrophages NLRP3 inflammasome and induce cell autophagy and apoptosis.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a210852.pdfacinetobacter baumannii|outer membrane vesicles|inflammation|autophagy|apoptosis |
| spellingShingle | FU Shixiang, XI Yue, DING Longkun, YAN Man, ZHAO Jun, JIAO Yudong, WU Liang Acinetobacter baumannii outer membrane vesicles induced inflammation of human monocyte-derived macrophages THP-1 Jichu yixue yu linchuang acinetobacter baumannii|outer membrane vesicles|inflammation|autophagy|apoptosis |
| title | Acinetobacter baumannii outer membrane vesicles induced inflammation of human monocyte-derived macrophages THP-1 |
| title_full | Acinetobacter baumannii outer membrane vesicles induced inflammation of human monocyte-derived macrophages THP-1 |
| title_fullStr | Acinetobacter baumannii outer membrane vesicles induced inflammation of human monocyte-derived macrophages THP-1 |
| title_full_unstemmed | Acinetobacter baumannii outer membrane vesicles induced inflammation of human monocyte-derived macrophages THP-1 |
| title_short | Acinetobacter baumannii outer membrane vesicles induced inflammation of human monocyte-derived macrophages THP-1 |
| title_sort | acinetobacter baumannii outer membrane vesicles induced inflammation of human monocyte derived macrophages thp 1 |
| topic | acinetobacter baumannii|outer membrane vesicles|inflammation|autophagy|apoptosis |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a210852.pdf |
| work_keys_str_mv | AT fushixiangxiyuedinglongkunyanmanzhaojunjiaoyudongwuliang acinetobacterbaumanniioutermembranevesiclesinducedinflammationofhumanmonocytederivedmacrophagesthp1 |