Study of genetic structure of Rutilus frisii kutum in Golestan province coastal waters using microsatellite markers
To determine the genetic diversity of Rutilus frisii kutum, 25 samples were collected from Gharesou and Gorgan-rud rivers in April 2008. The genomic DNA was extracted from the caudal fin using phenol- chloroform method and investigated with 10 microsatellite loci. All the 10 investigated loci were p...
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| Format: | Article |
| Language: | fas |
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Iranian Fisheries Research Organization
2010-01-01
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| Series: | Majallah-i ̒Ilmī-i Shīlāt-i Īrān |
| Subjects: | |
| Online Access: | http://isfj.areo.ir/article_116548_en.html |
| _version_ | 1818790090244096000 |
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| author | M. Rezaei; A. Shabani; B. Shabanpor; H. Keshiri |
| author_facet | M. Rezaei; A. Shabani; B. Shabanpor; H. Keshiri |
| author_sort | M. Rezaei; A. Shabani; B. Shabanpor; H. Keshiri |
| collection | DOAJ |
| description | To determine the genetic diversity of Rutilus frisii kutum, 25 samples were collected from Gharesou and Gorgan-rud rivers in April 2008. The genomic DNA was extracted from the caudal fin using phenol- chloroform method and investigated with 10 microsatellite loci. All the 10 investigated loci were polymorphic and showed variability in the two populations of the rivers. The mean number of allele per locus at population level and the observed heterozygosity ranged from 4 to 13 and 0.22 to 1.00, respectively. The FST value between populations was 0.014 (P<0.05), and the analysis of genetic variation distribution indicated a very high within population variation (99%) while among population variation is low (1%). Highly significant deviation from hardy-Weinberg equilibrium was observed in both populations which can be attributed either to the presence of null alleles or to the genetic bottleneck. The results indicated a high level of genetic variation in this species and we concluded that ..... |
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| format | Article |
| id | doaj.art-afc6e2ecec5245ac8dce15d6ea2e70e9 |
| institution | Directory Open Access Journal |
| issn | 1026-1354 2322-5998 |
| language | fas |
| last_indexed | 2024-12-18T14:49:55Z |
| publishDate | 2010-01-01 |
| publisher | Iranian Fisheries Research Organization |
| record_format | Article |
| series | Majallah-i ̒Ilmī-i Shīlāt-i Īrān |
| spelling | doaj.art-afc6e2ecec5245ac8dce15d6ea2e70e92022-12-21T21:04:11ZfasIranian Fisheries Research OrganizationMajallah-i ̒Ilmī-i Shīlāt-i Īrān1026-13542322-59982010-01-01193151156Study of genetic structure of Rutilus frisii kutum in Golestan province coastal waters using microsatellite markersM. Rezaei; A. Shabani; B. Shabanpor; H. KeshiriTo determine the genetic diversity of Rutilus frisii kutum, 25 samples were collected from Gharesou and Gorgan-rud rivers in April 2008. The genomic DNA was extracted from the caudal fin using phenol- chloroform method and investigated with 10 microsatellite loci. All the 10 investigated loci were polymorphic and showed variability in the two populations of the rivers. The mean number of allele per locus at population level and the observed heterozygosity ranged from 4 to 13 and 0.22 to 1.00, respectively. The FST value between populations was 0.014 (P<0.05), and the analysis of genetic variation distribution indicated a very high within population variation (99%) while among population variation is low (1%). Highly significant deviation from hardy-Weinberg equilibrium was observed in both populations which can be attributed either to the presence of null alleles or to the genetic bottleneck. The results indicated a high level of genetic variation in this species and we concluded that .....http://isfj.areo.ir/article_116548_en.htmlgeneticDNAkutum |
| spellingShingle | M. Rezaei; A. Shabani; B. Shabanpor; H. Keshiri Study of genetic structure of Rutilus frisii kutum in Golestan province coastal waters using microsatellite markers Majallah-i ̒Ilmī-i Shīlāt-i Īrān genetic DNA kutum |
| title | Study of genetic structure of Rutilus frisii kutum in Golestan province coastal waters using microsatellite markers |
| title_full | Study of genetic structure of Rutilus frisii kutum in Golestan province coastal waters using microsatellite markers |
| title_fullStr | Study of genetic structure of Rutilus frisii kutum in Golestan province coastal waters using microsatellite markers |
| title_full_unstemmed | Study of genetic structure of Rutilus frisii kutum in Golestan province coastal waters using microsatellite markers |
| title_short | Study of genetic structure of Rutilus frisii kutum in Golestan province coastal waters using microsatellite markers |
| title_sort | study of genetic structure of rutilus frisii kutum in golestan province coastal waters using microsatellite markers |
| topic | genetic DNA kutum |
| url | http://isfj.areo.ir/article_116548_en.html |
| work_keys_str_mv | AT mrezaeiashabanibshabanporhkeshiri studyofgeneticstructureofrutilusfrisiikutumingolestanprovincecoastalwatersusingmicrosatellitemarkers |