Association of Uncoupling Protein 1 (UCP1) gene polymorphism with obesity: a case-control study
Abstract Background Obesity is one of the main causes of morbidity and mortality worldwide. More than 120 genes have been shown to be associated with obesity related phenotypes. The aim of this study was to determine the effect of selected genetic polymorphisms in Uncoupling protein 1 (UCP1) and Nie...
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| Language: | English |
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BMC
2018-11-01
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| Series: | BMC Medical Genetics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12881-018-0715-5 |
| _version_ | 1818905959586594816 |
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| author | Shahanas Chathoth Mona H. Ismail Chittibabu Vatte Cyril Cyrus Zhara Al Ali Khandaker Ahtesham Ahmed Sadananda Acharya Aisha Mohammed Al Barqi Amein Al Ali |
| author_facet | Shahanas Chathoth Mona H. Ismail Chittibabu Vatte Cyril Cyrus Zhara Al Ali Khandaker Ahtesham Ahmed Sadananda Acharya Aisha Mohammed Al Barqi Amein Al Ali |
| author_sort | Shahanas Chathoth |
| collection | DOAJ |
| description | Abstract Background Obesity is one of the main causes of morbidity and mortality worldwide. More than 120 genes have been shown to be associated with obesity related phenotypes. The aim of this study was to determine the effect of selected genetic polymorphisms in Uncoupling protein 1 (UCP1) and Niemann-Pick C1 (NPC1) genes in an obese population in Saudi Arabia. Methods The genotypes of rs1800592, rs10011540 and rs3811791 (UCP1 gene) and rs1805081 and rs1805082 (NPC1 gene) were determined in a total of 492 subjects using TaqMan chemistry by Real-time PCR. In addition, capillary sequencing assay was performed to identify two specific polymorphisms viz., rs45539933 (exon 2) and rs2270565 (exon 5) of UCP1 gene. Results A significant association of UCP1 polymorphisms rs1800592 [OR, 1.52 (1.10–2.08); p = 0.009] was observed in the obese cohort after adjusting with age, sex and type 2 diabetes. Further BMI based stratification revealed that this association was inconsistent with both moderate and extreme obese cohort. A significant association of UCP1 polymorphisms rs3811791 was observed only in the moderate-obese cohort [OR = 2.89 (1.33–6.25); p = 0.007] but not in the extreme-obese cohort indicating an overlying genetic complexity between moderate-obesity and extreme-obesity. The risk allele frequencies, which were higher in moderate-obese cohort, had abnormal HDL, LDL and triglyceride levels. Conclusion The rs1800592 and rs3811791 of UCP1 gene are associated with obesity in general and in the moderate-obese group in particular. The associated UCP1 polymorphisms in the moderate-obese group may regulate the impaired energy metabolism which plays a significant role in the initial stages of obesity. |
| first_indexed | 2024-12-19T21:31:37Z |
| format | Article |
| id | doaj.art-afcb277c6428401d93b1764a2751ad03 |
| institution | Directory Open Access Journal |
| issn | 1471-2350 |
| language | English |
| last_indexed | 2024-12-19T21:31:37Z |
| publishDate | 2018-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Medical Genetics |
| spelling | doaj.art-afcb277c6428401d93b1764a2751ad032022-12-21T20:04:55ZengBMCBMC Medical Genetics1471-23502018-11-0119111010.1186/s12881-018-0715-5Association of Uncoupling Protein 1 (UCP1) gene polymorphism with obesity: a case-control studyShahanas Chathoth0Mona H. Ismail1Chittibabu Vatte2Cyril Cyrus3Zhara Al Ali4Khandaker Ahtesham Ahmed5Sadananda Acharya6Aisha Mohammed Al Barqi7Amein Al Ali8Department of Genetic Research, Institute for Research and Medical Consultation, Imam Abdulrahman Bin Faisal UniversityDepartment of Internal Medicine, King Fahd Hospital of the University, Imam Abdulrahman Bin Faisal UniversityDepartment of Genetic Research, Institute for Research and Medical Consultation, Imam Abdulrahman Bin Faisal UniversityDepartment of Genetic Research, Institute for Research and Medical Consultation, Imam Abdulrahman Bin Faisal UniversityDepartment of Internal Medicine, King Fahd Hospital of the University, Imam Abdulrahman Bin Faisal UniversityDepartment of Pathology, University of AlabamaDepartment of Public Health, College of Public Health, Imam Abdulrahman Bin Faisal UniversityDepartment of Internal Medicine, King Fahd Hospital of the University, Imam Abdulrahman Bin Faisal UniversityDepartment of Biochemistry, Imam Abdulrahman Bin Faisal UniversityAbstract Background Obesity is one of the main causes of morbidity and mortality worldwide. More than 120 genes have been shown to be associated with obesity related phenotypes. The aim of this study was to determine the effect of selected genetic polymorphisms in Uncoupling protein 1 (UCP1) and Niemann-Pick C1 (NPC1) genes in an obese population in Saudi Arabia. Methods The genotypes of rs1800592, rs10011540 and rs3811791 (UCP1 gene) and rs1805081 and rs1805082 (NPC1 gene) were determined in a total of 492 subjects using TaqMan chemistry by Real-time PCR. In addition, capillary sequencing assay was performed to identify two specific polymorphisms viz., rs45539933 (exon 2) and rs2270565 (exon 5) of UCP1 gene. Results A significant association of UCP1 polymorphisms rs1800592 [OR, 1.52 (1.10–2.08); p = 0.009] was observed in the obese cohort after adjusting with age, sex and type 2 diabetes. Further BMI based stratification revealed that this association was inconsistent with both moderate and extreme obese cohort. A significant association of UCP1 polymorphisms rs3811791 was observed only in the moderate-obese cohort [OR = 2.89 (1.33–6.25); p = 0.007] but not in the extreme-obese cohort indicating an overlying genetic complexity between moderate-obesity and extreme-obesity. The risk allele frequencies, which were higher in moderate-obese cohort, had abnormal HDL, LDL and triglyceride levels. Conclusion The rs1800592 and rs3811791 of UCP1 gene are associated with obesity in general and in the moderate-obese group in particular. The associated UCP1 polymorphisms in the moderate-obese group may regulate the impaired energy metabolism which plays a significant role in the initial stages of obesity.http://link.springer.com/article/10.1186/s12881-018-0715-5UCP1NCP1Moderate-obeseExtreme-obeseDiabetesHDL |
| spellingShingle | Shahanas Chathoth Mona H. Ismail Chittibabu Vatte Cyril Cyrus Zhara Al Ali Khandaker Ahtesham Ahmed Sadananda Acharya Aisha Mohammed Al Barqi Amein Al Ali Association of Uncoupling Protein 1 (UCP1) gene polymorphism with obesity: a case-control study BMC Medical Genetics UCP1 NCP1 Moderate-obese Extreme-obese Diabetes HDL |
| title | Association of Uncoupling Protein 1 (UCP1) gene polymorphism with obesity: a case-control study |
| title_full | Association of Uncoupling Protein 1 (UCP1) gene polymorphism with obesity: a case-control study |
| title_fullStr | Association of Uncoupling Protein 1 (UCP1) gene polymorphism with obesity: a case-control study |
| title_full_unstemmed | Association of Uncoupling Protein 1 (UCP1) gene polymorphism with obesity: a case-control study |
| title_short | Association of Uncoupling Protein 1 (UCP1) gene polymorphism with obesity: a case-control study |
| title_sort | association of uncoupling protein 1 ucp1 gene polymorphism with obesity a case control study |
| topic | UCP1 NCP1 Moderate-obese Extreme-obese Diabetes HDL |
| url | http://link.springer.com/article/10.1186/s12881-018-0715-5 |
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