ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast
Mitochondrial division is important for mitochondrial distribution and function. Recent data have demonstrated that ER–mitochondria contacts mark mitochondrial division sites, but the molecular basis and functions of these contacts are not understood. Here we show that in yeast, the ER–mitochondria...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2013-05-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/00422 |
| _version_ | 1828167471015657472 |
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| author | Andrew Murley Laura L Lackner Christof Osman Matthew West Gia K Voeltz Peter Walter Jodi Nunnari |
| author_facet | Andrew Murley Laura L Lackner Christof Osman Matthew West Gia K Voeltz Peter Walter Jodi Nunnari |
| author_sort | Andrew Murley |
| collection | DOAJ |
| description | Mitochondrial division is important for mitochondrial distribution and function. Recent data have demonstrated that ER–mitochondria contacts mark mitochondrial division sites, but the molecular basis and functions of these contacts are not understood. Here we show that in yeast, the ER–mitochondria tethering complex, ERMES, and the highly conserved Miro GTPase, Gem1, are spatially and functionally linked to ER-associated mitochondrial division. Gem1 acts as a negative regulator of ER–mitochondria contacts, an activity required for the spatial resolution and distribution of newly generated mitochondrial tips following division. Previous data have demonstrated that ERMES localizes with a subset of actively replicating mitochondrial nucleoids. We show that mitochondrial division is spatially linked to nucleoids and that a majority of these nucleoids segregate prior to division, resulting in their distribution into newly generated tips in the mitochondrial network. Thus, we postulate that ER-associated division serves to link the distribution of mitochondria and mitochondrial nucleoids in cells. |
| first_indexed | 2024-04-12T02:18:18Z |
| format | Article |
| id | doaj.art-afcfbf8868544b1da1f95b827db1e1a3 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:18:18Z |
| publishDate | 2013-05-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-afcfbf8868544b1da1f95b827db1e1a32022-12-22T03:52:12ZengeLife Sciences Publications LtdeLife2050-084X2013-05-01210.7554/eLife.00422ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeastAndrew Murley0Laura L Lackner1Christof Osman2Matthew West3Gia K Voeltz4Peter Walter5Jodi Nunnari6Department of Molecular and Cellular Biology, University of California, Davis, Davis, United StatesDepartment of Molecular and Cellular Biology, University of California, Davis, Davis, United StatesDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, United StatesDepartment of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Boulder, United StatesDepartment of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Boulder, United StatesDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, United States; Howard Hughes Medical Institute, University of California, San Francisco, United StatesDepartment of Molecular and Cellular Biology, University of California, Davis, Davis, United StatesMitochondrial division is important for mitochondrial distribution and function. Recent data have demonstrated that ER–mitochondria contacts mark mitochondrial division sites, but the molecular basis and functions of these contacts are not understood. Here we show that in yeast, the ER–mitochondria tethering complex, ERMES, and the highly conserved Miro GTPase, Gem1, are spatially and functionally linked to ER-associated mitochondrial division. Gem1 acts as a negative regulator of ER–mitochondria contacts, an activity required for the spatial resolution and distribution of newly generated mitochondrial tips following division. Previous data have demonstrated that ERMES localizes with a subset of actively replicating mitochondrial nucleoids. We show that mitochondrial division is spatially linked to nucleoids and that a majority of these nucleoids segregate prior to division, resulting in their distribution into newly generated tips in the mitochondrial network. Thus, we postulate that ER-associated division serves to link the distribution of mitochondria and mitochondrial nucleoids in cells.https://elifesciences.org/articles/00422ERMESGem1Miromitochondrial DNAmitochondria |
| spellingShingle | Andrew Murley Laura L Lackner Christof Osman Matthew West Gia K Voeltz Peter Walter Jodi Nunnari ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast eLife ERMES Gem1 Miro mitochondrial DNA mitochondria |
| title | ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast |
| title_full | ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast |
| title_fullStr | ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast |
| title_full_unstemmed | ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast |
| title_short | ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast |
| title_sort | er associated mitochondrial division links the distribution of mitochondria and mitochondrial dna in yeast |
| topic | ERMES Gem1 Miro mitochondrial DNA mitochondria |
| url | https://elifesciences.org/articles/00422 |
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